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Molecular genetic and immunohistochemical analysis of the tumor suppressor genes Rb and p53 in palmar and aggressive fibromatosis.

Publication ,  Journal Article
Muller, E; Castagnaro, M; Yandel, DW; Wolfe, HJ; Alman, BA
Published in: Diagn Mol Pathol
September 1996

This pilot project analyzed the tumor suppressor genes p53 and Rb in 13 cases of aggressive fibromatoses and six cases of palmar fibromatoses (Dupuytren contracture). Immunohistochemistry, reverse transcription polymerase chain reaction, polymerase chain reaction followed by single-strand confirmation polymorphism analysis, and Southern blot to detect gene rearrangements were used. No abnormalities were detected in p53. The aggressive fibromatoses demonstrated a lack of Rb immunohistochemical staining and decreased mRNA for Rb. No structural mutation in the coding sequence of the Rb gene was detected. The decreased level of Rb gene expression, despite a normal coding sequence, may indicate increased proliferation and may suggest potential treatment schemes.

Duke Scholars

Published In

Diagn Mol Pathol

DOI

ISSN

1052-9551

Publication Date

September 1996

Volume

5

Issue

3

Start / End Page

194 / 200

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Retinoblastoma Protein
  • RNA-Directed DNA Polymerase
  • Polymorphism, Single-Stranded Conformational
  • Polymerase Chain Reaction
  • Pathology
  • Immunohistochemistry
  • Humans
  • Genes, p53
  • Genes, Retinoblastoma
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Muller, E., Castagnaro, M., Yandel, D. W., Wolfe, H. J., & Alman, B. A. (1996). Molecular genetic and immunohistochemical analysis of the tumor suppressor genes Rb and p53 in palmar and aggressive fibromatosis. Diagn Mol Pathol, 5(3), 194–200. https://doi.org/10.1097/00019606-199609000-00008
Muller, E., M. Castagnaro, D. W. Yandel, H. J. Wolfe, and B. A. Alman. “Molecular genetic and immunohistochemical analysis of the tumor suppressor genes Rb and p53 in palmar and aggressive fibromatosis.Diagn Mol Pathol 5, no. 3 (September 1996): 194–200. https://doi.org/10.1097/00019606-199609000-00008.
Muller E, Castagnaro M, Yandel DW, Wolfe HJ, Alman BA. Molecular genetic and immunohistochemical analysis of the tumor suppressor genes Rb and p53 in palmar and aggressive fibromatosis. Diagn Mol Pathol. 1996 Sep;5(3):194–200.
Muller, E., et al. “Molecular genetic and immunohistochemical analysis of the tumor suppressor genes Rb and p53 in palmar and aggressive fibromatosis.Diagn Mol Pathol, vol. 5, no. 3, Sept. 1996, pp. 194–200. Pubmed, doi:10.1097/00019606-199609000-00008.
Muller E, Castagnaro M, Yandel DW, Wolfe HJ, Alman BA. Molecular genetic and immunohistochemical analysis of the tumor suppressor genes Rb and p53 in palmar and aggressive fibromatosis. Diagn Mol Pathol. 1996 Sep;5(3):194–200.

Published In

Diagn Mol Pathol

DOI

ISSN

1052-9551

Publication Date

September 1996

Volume

5

Issue

3

Start / End Page

194 / 200

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Retinoblastoma Protein
  • RNA-Directed DNA Polymerase
  • Polymorphism, Single-Stranded Conformational
  • Polymerase Chain Reaction
  • Pathology
  • Immunohistochemistry
  • Humans
  • Genes, p53
  • Genes, Retinoblastoma