ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.

Published

Journal Article (Review)

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a nonspecific DNA cleavage domain. ZFNs and TALENs enable a broad range of genetic modifications by inducing DNA double-strand breaks that stimulate error-prone nonhomologous end joining or homology-directed repair at specific genomic locations. Here, we review achievements made possible by site-specific nuclease technologies and discuss applications of these reagents for genetic analysis and manipulation. In addition, we highlight the therapeutic potential of ZFNs and TALENs and discuss future prospects for the field, including the emergence of clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases.

Full Text

Duke Authors

Cited Authors

  • Gaj, T; Gersbach, CA; Barbas, CF

Published Date

  • July 2013

Published In

Volume / Issue

  • 31 / 7

Start / End Page

  • 397 - 405

PubMed ID

  • 23664777

Pubmed Central ID

  • 23664777

Electronic International Standard Serial Number (EISSN)

  • 1879-3096

International Standard Serial Number (ISSN)

  • 0167-7799

Digital Object Identifier (DOI)

  • 10.1016/j.tibtech.2013.04.004

Language

  • eng