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ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.

Publication ,  Journal Article
Gaj, T; Gersbach, CA; Barbas, CF
Published in: Trends in biotechnology
July 2013

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a nonspecific DNA cleavage domain. ZFNs and TALENs enable a broad range of genetic modifications by inducing DNA double-strand breaks that stimulate error-prone nonhomologous end joining or homology-directed repair at specific genomic locations. Here, we review achievements made possible by site-specific nuclease technologies and discuss applications of these reagents for genetic analysis and manipulation. In addition, we highlight the therapeutic potential of ZFNs and TALENs and discuss future prospects for the field, including the emergence of clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases.

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Published In

Trends in biotechnology

DOI

EISSN

1879-3096

ISSN

0167-7799

Publication Date

July 2013

Volume

31

Issue

7

Start / End Page

397 / 405

Related Subject Headings

  • Zinc Fingers
  • Recombinant Proteins
  • Molecular Biology
  • Genetic Engineering
  • Deoxyribonucleases
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Biotechnology
  • Biological Therapy
  • 3206 Medical biotechnology
  • 3106 Industrial biotechnology
 

Citation

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ICMJE
MLA
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Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013). ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends in Biotechnology, 31(7), 397–405. https://doi.org/10.1016/j.tibtech.2013.04.004
Gaj, Thomas, Charles A. Gersbach, and Carlos F. Barbas. “ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.Trends in Biotechnology 31, no. 7 (July 2013): 397–405. https://doi.org/10.1016/j.tibtech.2013.04.004.
Gaj T, Gersbach CA, Barbas CF. ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends in biotechnology. 2013 Jul;31(7):397–405.
Gaj, Thomas, et al. “ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.Trends in Biotechnology, vol. 31, no. 7, July 2013, pp. 397–405. Epmc, doi:10.1016/j.tibtech.2013.04.004.
Gaj T, Gersbach CA, Barbas CF. ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends in biotechnology. 2013 Jul;31(7):397–405.
Journal cover image

Published In

Trends in biotechnology

DOI

EISSN

1879-3096

ISSN

0167-7799

Publication Date

July 2013

Volume

31

Issue

7

Start / End Page

397 / 405

Related Subject Headings

  • Zinc Fingers
  • Recombinant Proteins
  • Molecular Biology
  • Genetic Engineering
  • Deoxyribonucleases
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Biotechnology
  • Biological Therapy
  • 3206 Medical biotechnology
  • 3106 Industrial biotechnology