RNA-guided gene activation by CRISPR-Cas9-based transcription factors

Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.

Full Text

Duke Authors

Cited Authors

  • Perez-Pinera, P; Kocak, DD; Vockley, CM; Adler, AF; Kabadi, AM; Polstein, LR; Thakore, PI; Glass, KA; Ousterout, DG; Leong, KW; Guilak, F; Crawford, GE; Reddy, TE; Gersbach, CA

Published Date

  • 2013

Published In

Volume / Issue

  • 10 / 10

Start / End Page

  • 973 - 976

International Standard Serial Number (ISSN)

  • 1548-7091

Digital Object Identifier (DOI)

  • 10.1038/nmeth.2600

Citation Source

  • SciVal