RNA-guided gene activation by CRISPR-Cas9-based transcription factors
Publication
, Journal Article
Perez-Pinera, P; Kocak, DD; Vockley, CM; Adler, AF; Kabadi, AM; Polstein, LR; Thakore, PI; Glass, KA; Ousterout, DG; Leong, KW; Guilak, F ...
Published in: Nature Methods
2013
Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.
Duke Scholars
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Published In
Nature Methods
DOI
ISSN
1548-7091
Publication Date
2013
Volume
10
Issue
10
Start / End Page
973 / 976
Related Subject Headings
- Developmental Biology
- 11 Medical and Health Sciences
- 10 Technology
- 06 Biological Sciences
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Chicago
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NLM
Perez-Pinera, P., Kocak, D. D., Vockley, C. M., Adler, A. F., Kabadi, A. M., Polstein, L. R., … Gersbach, C. A. (2013). RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nature Methods, 10(10), 973–976. https://doi.org/10.1038/nmeth.2600
Perez-Pinera, P., D. D. Kocak, C. M. Vockley, A. F. Adler, A. M. Kabadi, L. R. Polstein, P. I. Thakore, et al. “RNA-guided gene activation by CRISPR-Cas9-based transcription factors.” Nature Methods 10, no. 10 (2013): 973–76. https://doi.org/10.1038/nmeth.2600.
Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, et al. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nature Methods. 2013;10(10):973–6.
Perez-Pinera, P., et al. “RNA-guided gene activation by CRISPR-Cas9-based transcription factors.” Nature Methods, vol. 10, no. 10, 2013, pp. 973–76. Scival, doi:10.1038/nmeth.2600.
Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nature Methods. 2013;10(10):973–976.
Published In
Nature Methods
DOI
ISSN
1548-7091
Publication Date
2013
Volume
10
Issue
10
Start / End Page
973 / 976
Related Subject Headings
- Developmental Biology
- 11 Medical and Health Sciences
- 10 Technology
- 06 Biological Sciences