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A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

Publication ,  Journal Article
Polstein, LR; Gersbach, CA
Published in: Nature Chemical Biology
January 1, 2015
Published version (DOI)

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.

Duke Scholars

Charles Gersbach
Author Charles Gersbach Biomedical Engineering
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Published In

Nature Chemical Biology

DOI

10.1038/nchembio.1753

EISSN

1552-4469

ISSN

1552-4450

Publication Date

January 1, 2015

Volume

11

Issue

3

Start / End Page

198 / 200

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry
 

Citation

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Polstein, L. R., & Gersbach, C. A. (2015). A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature Chemical Biology, 11(3), 198–200. https://doi.org/10.1038/nchembio.1753
Polstein, L. R., and C. A. Gersbach. “A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.” Nature Chemical Biology 11, no. 3 (January 1, 2015): 198–200. https://doi.org/10.1038/nchembio.1753.
Polstein LR, Gersbach CA. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature Chemical Biology. 2015 Jan 1;11(3):198–200.
Polstein, L. R., and C. A. Gersbach. “A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.” Nature Chemical Biology, vol. 11, no. 3, Jan. 2015, pp. 198–200. Scopus, doi:10.1038/nchembio.1753.
Polstein LR, Gersbach CA. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature Chemical Biology. 2015 Jan 1;11(3):198–200.

Published In

Nature Chemical Biology

DOI

10.1038/nchembio.1753

EISSN

1552-4469

ISSN

1552-4450

Publication Date

January 1, 2015

Volume

11

Issue

3

Start / End Page

198 / 200

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry