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A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

Publication ,  Journal Article
Polstein, LR; Gersbach, CA
Published in: Nature Chemical Biology
January 1, 2015

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.

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Published In

Nature Chemical Biology

DOI

EISSN

1552-4469

ISSN

1552-4450

Publication Date

January 1, 2015

Volume

11

Issue

3

Start / End Page

198 / 200

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry
 

Citation

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Polstein, L. R., & Gersbach, C. A. (2015). A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature Chemical Biology, 11(3), 198–200. https://doi.org/10.1038/nchembio.1753
Polstein, L. R., and C. A. Gersbach. “A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.” Nature Chemical Biology 11, no. 3 (January 1, 2015): 198–200. https://doi.org/10.1038/nchembio.1753.
Polstein LR, Gersbach CA. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature Chemical Biology. 2015 Jan 1;11(3):198–200.
Polstein, L. R., and C. A. Gersbach. “A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.” Nature Chemical Biology, vol. 11, no. 3, Jan. 2015, pp. 198–200. Scopus, doi:10.1038/nchembio.1753.
Polstein LR, Gersbach CA. A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nature Chemical Biology. 2015 Jan 1;11(3):198–200.

Published In

Nature Chemical Biology

DOI

EISSN

1552-4469

ISSN

1552-4450

Publication Date

January 1, 2015

Volume

11

Issue

3

Start / End Page

198 / 200

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry