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Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue.

Publication ,  Journal Article
Maher, JR; Chuchuen, O; Henderson, MH; Kim, S; Rinehart, MT; Kashuba, ADM; Wax, A; Katz, DF
Published in: Biomedical optics express
June 2015

We report the development of a combined confocal Raman spectroscopy (CRS) and optical coherence tomography (OCT) instrument (CRS-OCT) capable of measuring analytes in targeted biological tissues with sub-100-micron spatial resolution. The OCT subsystem was used to measure depth-resolved tissue morphology and guide the acquisition of chemically-specific Raman spectra. To demonstrate its utility, the instrument was used to accurately measure depth-resolved, physiologically-relevant concentrations of Tenofovir, a microbicide drug used to prevent the sexual transmission of HIV, in ex vivo tissue samples.

Duke Scholars

Published In

Biomedical optics express

DOI

EISSN

2156-7085

ISSN

2156-7085

Publication Date

June 2015

Volume

6

Issue

6

Start / End Page

2022 / 2035

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics
 

Citation

APA
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ICMJE
MLA
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Maher, J. R., Chuchuen, O., Henderson, M. H., Kim, S., Rinehart, M. T., Kashuba, A. D. M., … Katz, D. F. (2015). Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue. Biomedical Optics Express, 6(6), 2022–2035. https://doi.org/10.1364/boe.6.002022
Maher, Jason R., Oranat Chuchuen, Marcus H. Henderson, Sanghoon Kim, Matthew T. Rinehart, Angela D. M. Kashuba, Adam Wax, and David F. Katz. “Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue.Biomedical Optics Express 6, no. 6 (June 2015): 2022–35. https://doi.org/10.1364/boe.6.002022.
Maher JR, Chuchuen O, Henderson MH, Kim S, Rinehart MT, Kashuba ADM, et al. Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue. Biomedical optics express. 2015 Jun;6(6):2022–35.
Maher, Jason R., et al. “Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue.Biomedical Optics Express, vol. 6, no. 6, June 2015, pp. 2022–35. Epmc, doi:10.1364/boe.6.002022.
Maher JR, Chuchuen O, Henderson MH, Kim S, Rinehart MT, Kashuba ADM, Wax A, Katz DF. Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue. Biomedical optics express. 2015 Jun;6(6):2022–2035.
Journal cover image

Published In

Biomedical optics express

DOI

EISSN

2156-7085

ISSN

2156-7085

Publication Date

June 2015

Volume

6

Issue

6

Start / End Page

2022 / 2035

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics