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Real-time digital signal processing in multiphoton and time-resolved microscopy

Publication ,  Conference
Wilson, JW; Warren, WS; Fischer, MC
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
January 1, 2016

The use of multiphoton interactions in biological tissue for imaging contrast requires highly sensitive optical measurements. These often involve signal processing and filtering steps between the photodetector and the data acquisition device, such as photon counting and lock-in amplification. These steps can be implemented as real-time digital signal processing (DSP) elements on field-programmable gate array (FPGA) devices, an approach that affords much greater flexibility than commercial photon counting or lock-in devices. We will present progress toward developing two new FPGA-based DSP devices for multiphoton and time-resolved microscopy applications. The first is a high-speed multiharmonic lock-in amplifier for transient absorption microscopy, which is being developed for real-time analysis of the intensity-dependence of melanin, with applications in vivo and ex vivo (noninvasive histopathology of melanoma and pigmented lesions). The second device is a kHz lock-in amplifier running on a low cost ($50 - $200) development platform. It is our hope that these FPGA-based DSP devices will enable new, high-speed, low-cost applications in multiphoton and time-resolved microscopy.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628419375

Publication Date

January 1, 2016

Volume

9703
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wilson, J. W., Warren, W. S., & Fischer, M. C. (2016). Real-time digital signal processing in multiphoton and time-resolved microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9703). https://doi.org/10.1117/12.2218102
Wilson, J. W., W. S. Warren, and M. C. Fischer. “Real-time digital signal processing in multiphoton and time-resolved microscopy.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 9703, 2016. https://doi.org/10.1117/12.2218102.
Wilson JW, Warren WS, Fischer MC. Real-time digital signal processing in multiphoton and time-resolved microscopy. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2016.
Wilson, J. W., et al. “Real-time digital signal processing in multiphoton and time-resolved microscopy.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9703, 2016. Scopus, doi:10.1117/12.2218102.
Wilson JW, Warren WS, Fischer MC. Real-time digital signal processing in multiphoton and time-resolved microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2016.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628419375

Publication Date

January 1, 2016

Volume

9703