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Quantifying dorsal closure in three dimensions.

Publication ,  Journal Article
Lu, H; Sokolow, A; Kiehart, DP; Edwards, GS
Published in: Molecular biology of the cell
December 2016

Dorsal closure is an essential stage of Drosophila embryogenesis and is a powerful model system for morphogenesis, wound healing, and tissue biomechanics. During closure, two flanks of lateral epidermis close an eye-shaped dorsal opening that is filled with amnioserosa. The two flanks of lateral epidermis are zipped together at each canthus ("corner" of the eye). Actomyosin-rich purse strings are localized at each of the two leading edges of lateral epidermis ("lids" of the eye). Here we report that each purse string indents the dorsal surface at each leading edge. The amnioserosa tissue bulges outward during the early-to-mid stages of closure to form a remarkably smooth, asymmetric dome indicative of an isotropic and uniform surface tension. Internal pressure of the embryo and tissue elastic properties help to shape the dorsal surface.

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Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

December 2016

Volume

27

Issue

25

Start / End Page

3948 / 3955

Related Subject Headings

  • Morphogenesis
  • Models, Biological
  • Male
  • Imaging, Three-Dimensional
  • Hydrodynamics
  • Female
  • Epidermis
  • Embryonic Development
  • Embryo, Nonmammalian
  • Drosophila Proteins
 

Citation

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Lu, H., Sokolow, A., Kiehart, D. P., & Edwards, G. S. (2016). Quantifying dorsal closure in three dimensions. Molecular Biology of the Cell, 27(25), 3948–3955. https://doi.org/10.1091/mbc.e16-06-0400
Lu, Heng, Adam Sokolow, Daniel P. Kiehart, and Glenn S. Edwards. “Quantifying dorsal closure in three dimensions.Molecular Biology of the Cell 27, no. 25 (December 2016): 3948–55. https://doi.org/10.1091/mbc.e16-06-0400.
Lu H, Sokolow A, Kiehart DP, Edwards GS. Quantifying dorsal closure in three dimensions. Molecular biology of the cell. 2016 Dec;27(25):3948–55.
Lu, Heng, et al. “Quantifying dorsal closure in three dimensions.Molecular Biology of the Cell, vol. 27, no. 25, Dec. 2016, pp. 3948–55. Epmc, doi:10.1091/mbc.e16-06-0400.
Lu H, Sokolow A, Kiehart DP, Edwards GS. Quantifying dorsal closure in three dimensions. Molecular biology of the cell. 2016 Dec;27(25):3948–3955.

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

December 2016

Volume

27

Issue

25

Start / End Page

3948 / 3955

Related Subject Headings

  • Morphogenesis
  • Models, Biological
  • Male
  • Imaging, Three-Dimensional
  • Hydrodynamics
  • Female
  • Epidermis
  • Embryonic Development
  • Embryo, Nonmammalian
  • Drosophila Proteins