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Heparin mimicking polymer promotes myogenic differentiation of muscle progenitor cells.

Publication ,  Journal Article
Sangaj, N; Kyriakakis, P; Yang, D; Chang, C-W; Arya, G; Varghese, S
Published in: Biomacromolecules
December 13, 2010

Heparin and heparan sulfate mediated basic fibroblast growth factor (bFGF) signaling plays an important role in skeletal muscle homeostasis by maintaining a balance between proliferation and differentiation of muscle progenitor cells. In this study we investigate the role of a synthetic mimic of heparin, poly(sodium-4-styrenesulfonate) (PSS), on myogenic differentiation of C2C12 cells. Exogenous supplementation of PSS increased the differentiation of C2C12 cells in a dose-dependent manner, while the formation of multinucleated myotubes exhibited a nonmonotonic dependence with the concentration of PSS. Our results further suggest that one possible mechanism by which PSS promotes myogenic differentiation is by downregulating the mitogen activated extracellular regulated signaling kinase (MAPK/ERK) pathway. The binding ability of PSS to bFGF was found to be comparable to heparin through molecular docking calculations and by native PAGE. Such synthetic heparin mimics could offer a cost-effective alternative to heparin and also reduce the risk associated with batch-to-batch variation and contamination of heparin.

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Published In

Biomacromolecules

DOI

EISSN

1526-4602

Publication Date

December 13, 2010

Volume

11

Issue

12

Start / End Page

3294 / 3300

Location

United States

Related Subject Headings

  • Sulfonic Acids
  • Stem Cells
  • Polymers
  • Polymers
  • Myoblasts
  • Muscle Development
  • Muscle Cells
  • Molecular Mimicry
  • MAP Kinase Signaling System
  • Humans
 

Citation

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ICMJE
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Sangaj, N., Kyriakakis, P., Yang, D., Chang, C.-W., Arya, G., & Varghese, S. (2010). Heparin mimicking polymer promotes myogenic differentiation of muscle progenitor cells. Biomacromolecules, 11(12), 3294–3300. https://doi.org/10.1021/bm101041f
Sangaj, Nivedita, Phillip Kyriakakis, Darren Yang, Chien-Wen Chang, Gaurav Arya, and Shyni Varghese. “Heparin mimicking polymer promotes myogenic differentiation of muscle progenitor cells.Biomacromolecules 11, no. 12 (December 13, 2010): 3294–3300. https://doi.org/10.1021/bm101041f.
Sangaj N, Kyriakakis P, Yang D, Chang C-W, Arya G, Varghese S. Heparin mimicking polymer promotes myogenic differentiation of muscle progenitor cells. Biomacromolecules. 2010 Dec 13;11(12):3294–300.
Sangaj, Nivedita, et al. “Heparin mimicking polymer promotes myogenic differentiation of muscle progenitor cells.Biomacromolecules, vol. 11, no. 12, Dec. 2010, pp. 3294–300. Pubmed, doi:10.1021/bm101041f.
Sangaj N, Kyriakakis P, Yang D, Chang C-W, Arya G, Varghese S. Heparin mimicking polymer promotes myogenic differentiation of muscle progenitor cells. Biomacromolecules. 2010 Dec 13;11(12):3294–3300.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

Publication Date

December 13, 2010

Volume

11

Issue

12

Start / End Page

3294 / 3300

Location

United States

Related Subject Headings

  • Sulfonic Acids
  • Stem Cells
  • Polymers
  • Polymers
  • Myoblasts
  • Muscle Development
  • Muscle Cells
  • Molecular Mimicry
  • MAP Kinase Signaling System
  • Humans