Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.
Publication
, Journal Article
Keller, KE; Bhattacharya, SK; Borrás, T; Brunner, TM; Chansangpetch, S; Clark, AF; Dismuke, WM; Du, Y; Elliott, MH; Ethier, CR; Faralli, JA ...
Published in: Exp Eye Res
June 2018
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
Duke Scholars
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Published In
Exp Eye Res
DOI
EISSN
1096-0007
Publication Date
June 2018
Volume
171
Start / End Page
164 / 173
Location
England
Related Subject Headings
- Trabecular Meshwork
- Tissue and Organ Harvesting
- Tissue Preservation
- Tissue Donors
- Ophthalmology & Optometry
- Humans
- Guidelines as Topic
- Fetus
- Consensus
- Cell Separation
Citation
APA
Chicago
ICMJE
MLA
NLM
Keller, K. E., Bhattacharya, S. K., Borrás, T., Brunner, T. M., Chansangpetch, S., Clark, A. F., … Stamer, W. D. (2018). Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Exp Eye Res, 171, 164–173. https://doi.org/10.1016/j.exer.2018.03.001
Keller, Kate E., Sanjoy K. Bhattacharya, Theresa Borrás, Thomas M. Brunner, Sunee Chansangpetch, Abbott F. Clark, W Michael Dismuke, et al. “Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.” Exp Eye Res 171 (June 2018): 164–73. https://doi.org/10.1016/j.exer.2018.03.001.
Keller KE, Bhattacharya SK, Borrás T, Brunner TM, Chansangpetch S, Clark AF, et al. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Exp Eye Res. 2018 Jun;171:164–73.
Keller, Kate E., et al. “Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.” Exp Eye Res, vol. 171, June 2018, pp. 164–73. Pubmed, doi:10.1016/j.exer.2018.03.001.
Keller KE, Bhattacharya SK, Borrás T, Brunner TM, Chansangpetch S, Clark AF, Dismuke WM, Du Y, Elliott MH, Ethier CR, Faralli JA, Freddo TF, Fuchshofer R, Giovingo M, Gong H, Gonzalez P, Huang A, Johnstone MA, Kaufman PL, Kelley MJ, Knepper PA, Kopczynski CC, Kuchtey JG, Kuchtey RW, Kuehn MH, Lieberman RL, Lin SC, Liton P, Liu Y, Lütjen-Drecoll E, Mao W, Masis-Solano M, McDonnell F, McDowell CM, Overby DR, Pattabiraman PP, Raghunathan VK, Rao PV, Rhee DJ, Chowdhury UR, Russell P, Samples JR, Schwartz D, Stubbs EB, Tamm ER, Tan JC, Toris CB, Torrejon KY, Vranka JA, Wirtz MK, Yorio T, Zhang J, Zode GS, Fautsch MP, Peters DM, Acott TS, Stamer WD. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Exp Eye Res. 2018 Jun;171:164–173.
Published In
Exp Eye Res
DOI
EISSN
1096-0007
Publication Date
June 2018
Volume
171
Start / End Page
164 / 173
Location
England
Related Subject Headings
- Trabecular Meshwork
- Tissue and Organ Harvesting
- Tissue Preservation
- Tissue Donors
- Ophthalmology & Optometry
- Humans
- Guidelines as Topic
- Fetus
- Consensus
- Cell Separation