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Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.

Publication ,  Journal Article
Keller, KE; Bhattacharya, SK; Borrás, T; Brunner, TM; Chansangpetch, S; Clark, AF; Dismuke, WM; Du, Y; Elliott, MH; Ethier, CR; Faralli, JA ...
Published in: Exp Eye Res
June 2018

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

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Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

June 2018

Volume

171

Start / End Page

164 / 173

Location

England

Related Subject Headings

  • Trabecular Meshwork
  • Tissue and Organ Harvesting
  • Tissue Preservation
  • Tissue Donors
  • Ophthalmology & Optometry
  • Humans
  • Guidelines as Topic
  • Fetus
  • Consensus
  • Cell Separation
 

Citation

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ICMJE
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Keller, K. E., Bhattacharya, S. K., Borrás, T., Brunner, T. M., Chansangpetch, S., Clark, A. F., … Stamer, W. D. (2018). Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Exp Eye Res, 171, 164–173. https://doi.org/10.1016/j.exer.2018.03.001
Keller, Kate E., Sanjoy K. Bhattacharya, Theresa Borrás, Thomas M. Brunner, Sunee Chansangpetch, Abbott F. Clark, W Michael Dismuke, et al. “Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.Exp Eye Res 171 (June 2018): 164–73. https://doi.org/10.1016/j.exer.2018.03.001.
Keller KE, Bhattacharya SK, Borrás T, Brunner TM, Chansangpetch S, Clark AF, et al. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Exp Eye Res. 2018 Jun;171:164–73.
Keller, Kate E., et al. “Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.Exp Eye Res, vol. 171, June 2018, pp. 164–73. Pubmed, doi:10.1016/j.exer.2018.03.001.
Keller KE, Bhattacharya SK, Borrás T, Brunner TM, Chansangpetch S, Clark AF, Dismuke WM, Du Y, Elliott MH, Ethier CR, Faralli JA, Freddo TF, Fuchshofer R, Giovingo M, Gong H, Gonzalez P, Huang A, Johnstone MA, Kaufman PL, Kelley MJ, Knepper PA, Kopczynski CC, Kuchtey JG, Kuchtey RW, Kuehn MH, Lieberman RL, Lin SC, Liton P, Liu Y, Lütjen-Drecoll E, Mao W, Masis-Solano M, McDonnell F, McDowell CM, Overby DR, Pattabiraman PP, Raghunathan VK, Rao PV, Rhee DJ, Chowdhury UR, Russell P, Samples JR, Schwartz D, Stubbs EB, Tamm ER, Tan JC, Toris CB, Torrejon KY, Vranka JA, Wirtz MK, Yorio T, Zhang J, Zode GS, Fautsch MP, Peters DM, Acott TS, Stamer WD. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Exp Eye Res. 2018 Jun;171:164–173.
Journal cover image

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

June 2018

Volume

171

Start / End Page

164 / 173

Location

England

Related Subject Headings

  • Trabecular Meshwork
  • Tissue and Organ Harvesting
  • Tissue Preservation
  • Tissue Donors
  • Ophthalmology & Optometry
  • Humans
  • Guidelines as Topic
  • Fetus
  • Consensus
  • Cell Separation