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Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties.

Publication ,  Journal Article
Joh, DY; Zimmers, Z; Avlani, M; Heggestad, JT; Aydin, HB; Ganson, N; Kumar, S; Fontes, CM; Achar, RK; Hershfield, MS; Hucknall, AM; Chilkoti, A
Published in: Adv Healthc Mater
April 2019

Poly(ethylene glycol) (PEG), a linear polymer known for its "stealth" properties, is commonly used to passivate the surface of biomedical implants and devices, and it is conjugated to biologic drugs to improve their pharmacokinetics. However, its antigenicity is a growing concern. Here, the antigenicity of PEG is investigated when assembled in a poly(oligoethylene glycol) methacrylate (POEGMA) "bottlebrush" configuration on a planar surface. Using ethylene glycol (EG) repeat lengths of the POEGMA sidechains as a tunable parameter for optimization, POEGMA brushes with sidechain lengths of two and three EG repeats are identified as the optimal polymer architecture to minimize binding of anti-PEG antibodies (APAs), while retaining resistance to nonspecific binding by bovine serum albumin and cultured cells. Binding of backbone- versus endgroup-selective APAs to POEGMA brushes is further investigated, and finally the antigenicity of POEGMA coatings is assessed against APA-positive clinical plasma samples. These results are applied toward fabricating immunoassays on POEGMA surfaces with minimal reactivity toward APAs while retaining a low limit-of-detection for the analyte. Taken together, these results offer useful design concepts to reduce the antigenicity of polymer brush-based surface coatings used in applications involving human or animal matrices.

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Published In

Adv Healthc Mater

DOI

EISSN

2192-2659

Publication Date

April 2019

Volume

8

Issue

8

Start / End Page

e1801177

Location

Germany

Related Subject Headings

  • Surface Properties
  • Serum Albumin, Bovine
  • Prostheses and Implants
  • Polyethylene Glycols
  • NIH 3T3 Cells
  • Mice
  • Coated Materials, Biocompatible
  • Antigens
  • Antibodies
  • Animals
 

Citation

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Joh, D. Y., Zimmers, Z., Avlani, M., Heggestad, J. T., Aydin, H. B., Ganson, N., … Chilkoti, A. (2019). Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties. Adv Healthc Mater, 8(8), e1801177. https://doi.org/10.1002/adhm.201801177
Joh, Daniel Y., Zackary Zimmers, Manav Avlani, Jacob T. Heggestad, Hakan B. Aydin, Nancy Ganson, Shourya Kumar, et al. “Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties.Adv Healthc Mater 8, no. 8 (April 2019): e1801177. https://doi.org/10.1002/adhm.201801177.
Joh DY, Zimmers Z, Avlani M, Heggestad JT, Aydin HB, Ganson N, et al. Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties. Adv Healthc Mater. 2019 Apr;8(8):e1801177.
Joh, Daniel Y., et al. “Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties.Adv Healthc Mater, vol. 8, no. 8, Apr. 2019, p. e1801177. Pubmed, doi:10.1002/adhm.201801177.
Joh DY, Zimmers Z, Avlani M, Heggestad JT, Aydin HB, Ganson N, Kumar S, Fontes CM, Achar RK, Hershfield MS, Hucknall AM, Chilkoti A. Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties. Adv Healthc Mater. 2019 Apr;8(8):e1801177.
Journal cover image

Published In

Adv Healthc Mater

DOI

EISSN

2192-2659

Publication Date

April 2019

Volume

8

Issue

8

Start / End Page

e1801177

Location

Germany

Related Subject Headings

  • Surface Properties
  • Serum Albumin, Bovine
  • Prostheses and Implants
  • Polyethylene Glycols
  • NIH 3T3 Cells
  • Mice
  • Coated Materials, Biocompatible
  • Antigens
  • Antibodies
  • Animals