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Versatile biomanufacturing through stimulus-responsive cell-material feedback.

Publication ,  Journal Article
Dai, Z; Lee, AJ; Roberts, S; Sysoeva, TA; Huang, S; Dzuricky, M; Yang, X; Zhang, X; Liu, Z; Chilkoti, A; You, L
Published in: Nature chemical biology
October 2019

Small-scale production of biologics has great potential for enhancing the accessibility of biomanufacturing. By exploiting cell-material feedback, we have designed a concise platform to achieve versatile production, analysis and purification of diverse proteins and protein complexes. The core of our technology is a microbial swarmbot, which consists of a stimulus-sensitive polymeric microcapsule encapsulating engineered bacteria. By sensing the confinement, the bacteria undergo programmed partial lysis at a high local density. Conversely, the encapsulating material shrinks responding to the changing chemical environment caused by cell growth, squeezing out the protein products released by bacterial lysis. This platform is then integrated with downstream modules to enable quantification of enzymatic kinetics, purification of diverse proteins, quantitative control of protein interactions and assembly of functional protein complexes and multienzyme metabolic pathways. Our work demonstrates the use of the cell-material feedback to engineer a modular and flexible platform with sophisticated yet well-defined programmed functions.

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Published In

Nature chemical biology

DOI

EISSN

1552-4469

ISSN

1552-4450

Publication Date

October 2019

Volume

15

Issue

10

Start / End Page

1017 / 1024

Related Subject Headings

  • Plasmids
  • Genetic Engineering
  • Gene Expression Regulation
  • Escherichia coli
  • Bioreactors
  • Bioengineering
  • Biochemistry & Molecular Biology
  • Bacterial Proteins
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
 

Citation

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Dai, Z., Lee, A. J., Roberts, S., Sysoeva, T. A., Huang, S., Dzuricky, M., … You, L. (2019). Versatile biomanufacturing through stimulus-responsive cell-material feedback. Nature Chemical Biology, 15(10), 1017–1024. https://doi.org/10.1038/s41589-019-0357-8
Dai, Zhuojun, Anna J. Lee, Stefan Roberts, Tatyana A. Sysoeva, Shuqiang Huang, Michael Dzuricky, Xiaoyu Yang, et al. “Versatile biomanufacturing through stimulus-responsive cell-material feedback.Nature Chemical Biology 15, no. 10 (October 2019): 1017–24. https://doi.org/10.1038/s41589-019-0357-8.
Dai Z, Lee AJ, Roberts S, Sysoeva TA, Huang S, Dzuricky M, et al. Versatile biomanufacturing through stimulus-responsive cell-material feedback. Nature chemical biology. 2019 Oct;15(10):1017–24.
Dai, Zhuojun, et al. “Versatile biomanufacturing through stimulus-responsive cell-material feedback.Nature Chemical Biology, vol. 15, no. 10, Oct. 2019, pp. 1017–24. Epmc, doi:10.1038/s41589-019-0357-8.
Dai Z, Lee AJ, Roberts S, Sysoeva TA, Huang S, Dzuricky M, Yang X, Zhang X, Liu Z, Chilkoti A, You L. Versatile biomanufacturing through stimulus-responsive cell-material feedback. Nature chemical biology. 2019 Oct;15(10):1017–1024.

Published In

Nature chemical biology

DOI

EISSN

1552-4469

ISSN

1552-4450

Publication Date

October 2019

Volume

15

Issue

10

Start / End Page

1017 / 1024

Related Subject Headings

  • Plasmids
  • Genetic Engineering
  • Gene Expression Regulation
  • Escherichia coli
  • Bioreactors
  • Bioengineering
  • Biochemistry & Molecular Biology
  • Bacterial Proteins
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology