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In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays.

Publication ,  Journal Article
Heggestad, JT; Fontes, CM; Joh, DY; Hucknall, AM; Chilkoti, A
Published in: Advanced materials (Deerfield Beach, Fla.)
January 2020

"Nonfouling" polymer brush surfaces can greatly improve the performance of in vitro diagnostic (IVD) assays due to the reduction of nonspecific protein adsorption and consequent improvement of signal-to-noise ratios. The development of synthetic polymer brush architectures that suppress adventitious protein adsorption is reviewed, and their integration into surface plasmon resonance and fluorescent sandwich immunoassay formats is discussed. Also, highlighted is a novel, self-contained immunoassay platform (the D4 assay) that transforms time-consuming laboratory-based assays into a user-friendly and point-of-care format with a sensitivity and specificity comparable or better than standard enzyme-linked immunosorbent assay (ELISA) directly from unprocessed samples. These advancements clearly demonstrate the utility of nonfouling polymer brushes as a substrate for ultrasensitive and robust diagnostic assays that may be suitable for clinical testing, in field and laboratory settings.

Duke Scholars

Published In

Advanced materials (Deerfield Beach, Fla.)

DOI

EISSN

1521-4095

ISSN

0935-9648

Publication Date

January 2020

Volume

32

Issue

2

Start / End Page

e1903285

Related Subject Headings

  • Polymers
  • Point-of-Care Testing
  • Nanoscience & Nanotechnology
  • Immunoassay
  • Humans
  • Biofouling
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
 

Citation

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Heggestad, J. T., Fontes, C. M., Joh, D. Y., Hucknall, A. M., & Chilkoti, A. (2020). In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays. Advanced Materials (Deerfield Beach, Fla.), 32(2), e1903285. https://doi.org/10.1002/adma.201903285
Heggestad, Jacob T., Cassio M. Fontes, Daniel Y. Joh, Angus M. Hucknall, and Ashutosh Chilkoti. “In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays.Advanced Materials (Deerfield Beach, Fla.) 32, no. 2 (January 2020): e1903285. https://doi.org/10.1002/adma.201903285.
Heggestad JT, Fontes CM, Joh DY, Hucknall AM, Chilkoti A. In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays. Advanced materials (Deerfield Beach, Fla). 2020 Jan;32(2):e1903285.
Heggestad, Jacob T., et al. “In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays.Advanced Materials (Deerfield Beach, Fla.), vol. 32, no. 2, Jan. 2020, p. e1903285. Epmc, doi:10.1002/adma.201903285.
Heggestad JT, Fontes CM, Joh DY, Hucknall AM, Chilkoti A. In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays. Advanced materials (Deerfield Beach, Fla). 2020 Jan;32(2):e1903285.
Journal cover image

Published In

Advanced materials (Deerfield Beach, Fla.)

DOI

EISSN

1521-4095

ISSN

0935-9648

Publication Date

January 2020

Volume

32

Issue

2

Start / End Page

e1903285

Related Subject Headings

  • Polymers
  • Point-of-Care Testing
  • Nanoscience & Nanotechnology
  • Immunoassay
  • Humans
  • Biofouling
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering