Imaging microtubules in buffer solution using tapping mode atomic force microscopy
Publication
, Conference
Fritz, M; Radmacher, M; Allersma, MW; Cleveland, JP; Stewart, RJ; Hansma, PK; Schmidt, CF
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 1995
Taxol stabilized microtubules were imaged in their native state in buffer solution without any fixation by an atomic force microscope (AFM) operated in tapping mode in liquids. Glass cover slips were derivatized with a positively charged silane to adsorb the filaments. The adsorbed microtubules could be imaged stably without any visible damage for hours. In cases where a microtubule crossed above another one, it often broke to follow the curvature of the underlying one. Longitudinal structures with a spacing of about 8 nm could be resolved suggesting they are protofilaments.
Duke Scholars
Published In
Proceedings of SPIE - The International Society for Optical Engineering
ISSN
0277-786X
ISBN
0819417319
Publication Date
January 1, 1995
Volume
2384
Start / End Page
150 / 157
Citation
APA
Chicago
ICMJE
MLA
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Fritz, M., Radmacher, M., Allersma, M. W., Cleveland, J. P., Stewart, R. J., Hansma, P. K., & Schmidt, C. F. (1995). Imaging microtubules in buffer solution using tapping mode atomic force microscopy. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2384, pp. 150–157).
Fritz, M., M. Radmacher, M. W. Allersma, J. P. Cleveland, R. J. Stewart, P. K. Hansma, and C. F. Schmidt. “Imaging microtubules in buffer solution using tapping mode atomic force microscopy.” In Proceedings of SPIE - The International Society for Optical Engineering, 2384:150–57, 1995.
Fritz M, Radmacher M, Allersma MW, Cleveland JP, Stewart RJ, Hansma PK, et al. Imaging microtubules in buffer solution using tapping mode atomic force microscopy. In: Proceedings of SPIE - The International Society for Optical Engineering. 1995. p. 150–7.
Fritz, M., et al. “Imaging microtubules in buffer solution using tapping mode atomic force microscopy.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 2384, 1995, pp. 150–57.
Fritz M, Radmacher M, Allersma MW, Cleveland JP, Stewart RJ, Hansma PK, Schmidt CF. Imaging microtubules in buffer solution using tapping mode atomic force microscopy. Proceedings of SPIE - The International Society for Optical Engineering. 1995. p. 150–157.
Published In
Proceedings of SPIE - The International Society for Optical Engineering
ISSN
0277-786X
ISBN
0819417319
Publication Date
January 1, 1995
Volume
2384
Start / End Page
150 / 157