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Imaging microtubules in buffer solution using tapping mode atomic force microscopy

Publication ,  Conference
Fritz, M; Radmacher, M; Allersma, MW; Cleveland, JP; Stewart, RJ; Hansma, PK; Schmidt, CF
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 1995

Taxol stabilized microtubules were imaged in their native state in buffer solution without any fixation by an atomic force microscope (AFM) operated in tapping mode in liquids. Glass cover slips were derivatized with a positively charged silane to adsorb the filaments. The adsorbed microtubules could be imaged stably without any visible damage for hours. In cases where a microtubule crossed above another one, it often broke to follow the curvature of the underlying one. Longitudinal structures with a spacing of about 8 nm could be resolved suggesting they are protofilaments.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

ISSN

0277-786X

Publication Date

January 1, 1995

Volume

2384

Start / End Page

150 / 157

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Fritz, M., Radmacher, M., Allersma, M. W., Cleveland, J. P., Stewart, R. J., Hansma, P. K., & Schmidt, C. F. (1995). Imaging microtubules in buffer solution using tapping mode atomic force microscopy. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2384, pp. 150–157).
Fritz, M., M. Radmacher, M. W. Allersma, J. P. Cleveland, R. J. Stewart, P. K. Hansma, and C. F. Schmidt. “Imaging microtubules in buffer solution using tapping mode atomic force microscopy.” In Proceedings of SPIE - The International Society for Optical Engineering, 2384:150–57, 1995.
Fritz M, Radmacher M, Allersma MW, Cleveland JP, Stewart RJ, Hansma PK, et al. Imaging microtubules in buffer solution using tapping mode atomic force microscopy. In: Proceedings of SPIE - The International Society for Optical Engineering. 1995. p. 150–7.
Fritz, M., et al. “Imaging microtubules in buffer solution using tapping mode atomic force microscopy.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 2384, 1995, pp. 150–57.
Fritz M, Radmacher M, Allersma MW, Cleveland JP, Stewart RJ, Hansma PK, Schmidt CF. Imaging microtubules in buffer solution using tapping mode atomic force microscopy. Proceedings of SPIE - The International Society for Optical Engineering. 1995. p. 150–157.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

ISSN

0277-786X

Publication Date

January 1, 1995

Volume

2384

Start / End Page

150 / 157

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering