Skip to main content
Journal cover image

Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities.

Publication ,  Journal Article
Cruz, DF; Fontes, CM; Semeniak, D; Huang, J; Hucknall, A; Chilkoti, A; Mikkelsen, MH
Published in: Nano letters
June 2020

Fluorescence-based microarrays are promising diagnostic tools due to their high throughput, small sample volume requirements, and multiplexing capabilities. However, their low fluorescence output has limited their implementation for in vitro diagnostics applications in point-of-care (POC) settings. Here, by integration of a sandwich immunoassay microarray within a plasmonic nanogap cavity, we demonstrate strongly enhanced fluorescence which is critical for readout by inexpensive POC detectors. The immunoassay consists of inkjet-printed antibodies on a polymer brush which is grown on a gold film. Colloidally synthesized silver nanocubes are placed on top and interact with the underlying gold film creating high local electromagnetic field enhancements. By varying the thickness of the brush from 5 to 20 nm, up to a 151-fold increase in fluorescence and 14-fold improvement in the limit-of-detection is observed for the cardiac biomarker B-type natriuretic peptide (BNP) compared to the unenhanced assay, paving the way for a new generation of POC clinical diagnostics.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nano letters

DOI

EISSN

1530-6992

ISSN

1530-6984

Publication Date

June 2020

Volume

20

Issue

6

Start / End Page

4330 / 4336

Related Subject Headings

  • Silver
  • Polymers
  • Point-of-Care Testing
  • Nanotechnology
  • Nanoscience & Nanotechnology
  • Immunoassay
  • Humans
  • Gold
  • Bioprinting
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cruz, D. F., Fontes, C. M., Semeniak, D., Huang, J., Hucknall, A., Chilkoti, A., & Mikkelsen, M. H. (2020). Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities. Nano Letters, 20(6), 4330–4336. https://doi.org/10.1021/acs.nanolett.0c01051
Cruz, Daniela F., Cassio M. Fontes, Daria Semeniak, Jiani Huang, Angus Hucknall, Ashutosh Chilkoti, and Maiken H. Mikkelsen. “Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities.Nano Letters 20, no. 6 (June 2020): 4330–36. https://doi.org/10.1021/acs.nanolett.0c01051.
Cruz DF, Fontes CM, Semeniak D, Huang J, Hucknall A, Chilkoti A, et al. Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities. Nano letters. 2020 Jun;20(6):4330–6.
Cruz, Daniela F., et al. “Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities.Nano Letters, vol. 20, no. 6, June 2020, pp. 4330–36. Epmc, doi:10.1021/acs.nanolett.0c01051.
Cruz DF, Fontes CM, Semeniak D, Huang J, Hucknall A, Chilkoti A, Mikkelsen MH. Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities. Nano letters. 2020 Jun;20(6):4330–4336.
Journal cover image

Published In

Nano letters

DOI

EISSN

1530-6992

ISSN

1530-6984

Publication Date

June 2020

Volume

20

Issue

6

Start / End Page

4330 / 4336

Related Subject Headings

  • Silver
  • Polymers
  • Point-of-Care Testing
  • Nanotechnology
  • Nanoscience & Nanotechnology
  • Immunoassay
  • Humans
  • Gold
  • Bioprinting