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Quantitative Jones matrix imaging using vectorial Fourier ptychography

Publication ,  Journal Article
Dai, X; Xu, S; Yang, X; Zhou, KC; Glass, C; Konda, PC; Horstmeyer, R
September 30, 2021

This paper presents a microscopic imaging technique that uses variable-angle illumination to recover the complex polarimetric properties of a specimen at high resolution and over a large field-of-view. The approach extends Fourier ptychography, which is a synthetic aperture-based imaging approach to improve resolution with phaseless measurements, to additionally account for the vectorial nature of light. After images are acquired using a standard microscope outfitted with an LED illumination array and two polarizers, our vectorial Fourier Ptychography (vFP) algorithm solves for the complex 2x2 Jones matrix of the anisotropic specimen of interest at each resolved spatial location. We introduce a new sequential Gauss-Newton-based solver that additionally jointly estimates and removes polarization-dependent imaging system aberrations. We demonstrate effective vFP performance by generating large-area (29 mm$^2$), high-resolution (1.24 $\mu$m full-pitch) reconstructions of sample absorption, phase, orientation, diattenuation, and retardance for a variety of calibration samples and biological specimens.

Duke Scholars

Publication Date

September 30, 2021
 

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Dai, X., Xu, S., Yang, X., Zhou, K. C., Glass, C., Konda, P. C., & Horstmeyer, R. (2021). Quantitative Jones matrix imaging using vectorial Fourier ptychography.
Dai, Xiang, Shiqi Xu, Xi Yang, Kevin C. Zhou, Carolyn Glass, Pavan Chandra Konda, and Roarke Horstmeyer. “Quantitative Jones matrix imaging using vectorial Fourier ptychography,” September 30, 2021.
Dai X, Xu S, Yang X, Zhou KC, Glass C, Konda PC, et al. Quantitative Jones matrix imaging using vectorial Fourier ptychography. 2021 Sep 30;
Dai X, Xu S, Yang X, Zhou KC, Glass C, Konda PC, Horstmeyer R. Quantitative Jones matrix imaging using vectorial Fourier ptychography. 2021 Sep 30;

Publication Date

September 30, 2021