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In Vivo Effects of Silver Nanoparticles on Development, Behavior, and Mitochondrial Function are Altered by Genetic Defects in Mitochondrial Dynamics.

Publication ,  Journal Article
Mello, DF; Maurer, LL; Ryde, IT; Songr, DH; Marinakos, SM; Jiang, C; Wiesner, MR; Hsu-Kim, H; Meyer, JN
Published in: Environmental science & technology
January 2022

Silver nanoparticles (AgNPs) are extensively used in consumer products and biomedical applications, thus guaranteeing both environmental and human exposures. Despite extensive research addressing AgNP safety, there are still major knowledge gaps regarding AgNP toxicity mechanisms, particularly in whole organisms. Mitochondrial dysfunction is frequently described as an important cytotoxicity mechanism for AgNPs; however, it is still unclear if mitochondria are the direct targets of AgNPs. To test this, we exposed the nematodeCaenorhabditis elegans to sublethal concentrations of AgNPs and assessed specific mitochondrial parameters as well as organismal-level endpoints that are highly reliant on mitochondrial function, such as development and chemotaxis behavior. All AgNPs tested significantly delayed nematode development, disrupted mitochondrial bioenergetics, and blocked chemotaxis. However, silver was not preferentially accumulated in mitochondria, indicating that these effects are likely not due to direct mitochondria-AgNP interactions. Mutant nematodes with deficiencies in mitochondrial dynamics displayed both greater and decreased susceptibility to AgNPs compared to wild-type nematodes, which was dependent on the assay and AgNP type. Our study suggests that AgNPs indirectly promote mitochondrial dysfunction, leading to adverse outcomes at the organismal level, and reveals a role of gene-environment interactions in the susceptibility to AgNPs. Finally, we propose a novel hypothetical adverse outcome pathway for AgNP effects to guide future research.

Duke Scholars

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Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

January 2022

Volume

56

Issue

2

Start / End Page

1113 / 1124

Related Subject Headings

  • Silver
  • Mitochondrial Dynamics
  • Mitochondria
  • Metal Nanoparticles
  • Humans
  • Environmental Sciences
 

Citation

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Mello, D. F., Maurer, L. L., Ryde, I. T., Songr, D. H., Marinakos, S. M., Jiang, C., … Meyer, J. N. (2022). In Vivo Effects of Silver Nanoparticles on Development, Behavior, and Mitochondrial Function are Altered by Genetic Defects in Mitochondrial Dynamics. Environmental Science & Technology, 56(2), 1113–1124. https://doi.org/10.1021/acs.est.1c05915
Mello, Danielle F., Laura L. Maurer, Ian T. Ryde, Dong Hoon Songr, Stella M. Marinakos, Chuanjia Jiang, Mark R. Wiesner, Heileen Hsu-Kim, and Joel N. Meyer. “In Vivo Effects of Silver Nanoparticles on Development, Behavior, and Mitochondrial Function are Altered by Genetic Defects in Mitochondrial Dynamics.Environmental Science & Technology 56, no. 2 (January 2022): 1113–24. https://doi.org/10.1021/acs.est.1c05915.
Mello DF, Maurer LL, Ryde IT, Songr DH, Marinakos SM, Jiang C, et al. In Vivo Effects of Silver Nanoparticles on Development, Behavior, and Mitochondrial Function are Altered by Genetic Defects in Mitochondrial Dynamics. Environmental science & technology. 2022 Jan;56(2):1113–24.
Mello, Danielle F., et al. “In Vivo Effects of Silver Nanoparticles on Development, Behavior, and Mitochondrial Function are Altered by Genetic Defects in Mitochondrial Dynamics.Environmental Science & Technology, vol. 56, no. 2, Jan. 2022, pp. 1113–24. Epmc, doi:10.1021/acs.est.1c05915.
Mello DF, Maurer LL, Ryde IT, Songr DH, Marinakos SM, Jiang C, Wiesner MR, Hsu-Kim H, Meyer JN. In Vivo Effects of Silver Nanoparticles on Development, Behavior, and Mitochondrial Function are Altered by Genetic Defects in Mitochondrial Dynamics. Environmental science & technology. 2022 Jan;56(2):1113–1124.
Journal cover image

Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

January 2022

Volume

56

Issue

2

Start / End Page

1113 / 1124

Related Subject Headings

  • Silver
  • Mitochondrial Dynamics
  • Mitochondria
  • Metal Nanoparticles
  • Humans
  • Environmental Sciences