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Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy.

Publication ,  Journal Article
Zhu, X; Huang, Q; DiSpirito, A; Vu, T; Rong, Q; Peng, X; Sheng, H; Shen, X; Zhou, Q; Jiang, L; Hoffmann, U; Yao, J
Published in: Light Sci Appl
May 17, 2022

High-speed high-resolution imaging of the whole-brain hemodynamics is critically important to facilitating neurovascular research. High imaging speed and image quality are crucial to visualizing real-time hemodynamics in complex brain vascular networks, and tracking fast pathophysiological activities at the microvessel level, which will enable advances in current queries in neurovascular and brain metabolism research, including stroke, dementia, and acute brain injury. Further, real-time imaging of oxygen saturation of hemoglobin (sO2) can capture fast-paced oxygen delivery dynamics, which is needed to solve pertinent questions in these fields and beyond. Here, we present a novel ultrafast functional photoacoustic microscopy (UFF-PAM) to image the whole-brain hemodynamics and oxygenation. UFF-PAM takes advantage of several key engineering innovations, including stimulated Raman scattering (SRS) based dual-wavelength laser excitation, water-immersible 12-facet-polygon scanner, high-sensitivity ultrasound transducer, and deep-learning-based image upsampling. A volumetric imaging rate of 2 Hz has been achieved over a field of view (FOV) of 11 × 7.5 × 1.5 mm3 with a high spatial resolution of ~10 μm. Using the UFF-PAM system, we have demonstrated proof-of-concept studies on the mouse brains in response to systemic hypoxia, sodium nitroprusside, and stroke. We observed the mouse brain's fast morphological and functional changes over the entire cortex, including vasoconstriction, vasodilation, and deoxygenation. More interestingly, for the first time, with the whole-brain FOV and micro-vessel resolution, we captured the vasoconstriction and hypoxia simultaneously in the spreading depolarization (SD) wave. We expect the new imaging technology will provide a great potential for fundamental brain research under various pathological and physiological conditions.

Duke Scholars

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Published In

Light Sci Appl

DOI

EISSN

2047-7538

Publication Date

May 17, 2022

Volume

11

Issue

1

Start / End Page

138

Location

England

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 0205 Optical Physics
 

Citation

APA
Chicago
ICMJE
MLA
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Zhu, X., Huang, Q., DiSpirito, A., Vu, T., Rong, Q., Peng, X., … Yao, J. (2022). Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy. Light Sci Appl, 11(1), 138. https://doi.org/10.1038/s41377-022-00836-2
Zhu, Xiaoyi, Qiang Huang, Anthony DiSpirito, Tri Vu, Qiangzhou Rong, Xiaorui Peng, Huaxin Sheng, et al. “Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy.Light Sci Appl 11, no. 1 (May 17, 2022): 138. https://doi.org/10.1038/s41377-022-00836-2.
Zhu X, Huang Q, DiSpirito A, Vu T, Rong Q, Peng X, et al. Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy. Light Sci Appl. 2022 May 17;11(1):138.
Zhu, Xiaoyi, et al. “Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy.Light Sci Appl, vol. 11, no. 1, May 2022, p. 138. Pubmed, doi:10.1038/s41377-022-00836-2.
Zhu X, Huang Q, DiSpirito A, Vu T, Rong Q, Peng X, Sheng H, Shen X, Zhou Q, Jiang L, Hoffmann U, Yao J. Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy. Light Sci Appl. 2022 May 17;11(1):138.

Published In

Light Sci Appl

DOI

EISSN

2047-7538

Publication Date

May 17, 2022

Volume

11

Issue

1

Start / End Page

138

Location

England

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 0205 Optical Physics