Skip to main content
Journal cover image

Programmable RNA sensing for cell monitoring and manipulation.

Publication ,  Journal Article
Qian, Y; Li, J; Zhao, S; Matthews, EA; Adoff, M; Zhong, W; An, X; Yeo, M; Park, C; Yang, X; Wang, B-S; Southwell, DG; Huang, ZJ
Published in: Nature
October 2022

RNA is a central and universal mediator of genetic information underlying the diversity of cell types and cell states, which together shape tissue organization and organismal function across species and lifespans. Despite numerous advances in RNA sequencing technologies and the massive accumulation of transcriptome datasets across the life sciences1,2, the dearth of technologies that use RNAs to observe and manipulate cell types remains a bottleneck in biology and medicine. Here we describe CellREADR (Cell access through RNA sensing by Endogenous ADAR), a programmable RNA-sensing technology that leverages RNA editing mediated by ADAR to couple the detection of cell-defining RNAs with the translation of effector proteins. Viral delivery of CellREADR conferred specific cell-type access in mouse and rat brains and in ex vivo human brain tissues. Furthermore, CellREADR enabled the recording and control of specific types of neurons in behaving mice. CellREADR thus highlights the potential for RNA-based monitoring and editing of animal cells in ways that are specific, versatile, simple and generalizable across organ systems and species, with wide applications in biology, biotechnology and programmable RNA medicine.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nature

DOI

EISSN

1476-4687

Publication Date

October 2022

Volume

610

Issue

7933

Start / End Page

713 / 721

Location

England

Related Subject Headings

  • Transcriptome
  • Sequence Analysis, RNA
  • Rats
  • RNA Editing
  • RNA
  • Protein Biosynthesis
  • Neurons
  • Mice
  • Humans
  • General Science & Technology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Qian, Y., Li, J., Zhao, S., Matthews, E. A., Adoff, M., Zhong, W., … Huang, Z. J. (2022). Programmable RNA sensing for cell monitoring and manipulation. Nature, 610(7933), 713–721. https://doi.org/10.1038/s41586-022-05280-1
Qian, Yongjun, Jiayun Li, Shengli Zhao, Elizabeth A. Matthews, Michael Adoff, Weixin Zhong, Xu An, et al. “Programmable RNA sensing for cell monitoring and manipulation.Nature 610, no. 7933 (October 2022): 713–21. https://doi.org/10.1038/s41586-022-05280-1.
Qian Y, Li J, Zhao S, Matthews EA, Adoff M, Zhong W, et al. Programmable RNA sensing for cell monitoring and manipulation. Nature. 2022 Oct;610(7933):713–21.
Qian, Yongjun, et al. “Programmable RNA sensing for cell monitoring and manipulation.Nature, vol. 610, no. 7933, Oct. 2022, pp. 713–21. Pubmed, doi:10.1038/s41586-022-05280-1.
Qian Y, Li J, Zhao S, Matthews EA, Adoff M, Zhong W, An X, Yeo M, Park C, Yang X, Wang B-S, Southwell DG, Huang ZJ. Programmable RNA sensing for cell monitoring and manipulation. Nature. 2022 Oct;610(7933):713–721.
Journal cover image

Published In

Nature

DOI

EISSN

1476-4687

Publication Date

October 2022

Volume

610

Issue

7933

Start / End Page

713 / 721

Location

England

Related Subject Headings

  • Transcriptome
  • Sequence Analysis, RNA
  • Rats
  • RNA Editing
  • RNA
  • Protein Biosynthesis
  • Neurons
  • Mice
  • Humans
  • General Science & Technology