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Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes.

Publication ,  Journal Article
Lerner, RS; Seiser, RM; Zheng, T; Lager, PJ; Reedy, MC; Keene, JD; Nicchitta, CV
Published in: RNA
September 2003

In eukaryotic cells, it is generally accepted that protein synthesis is compartmentalized; soluble proteins are synthesized on free ribosomes, whereas secretory and membrane proteins are synthesized on endoplasmic reticulum (ER)-bound ribosomes. The partitioning of mRNAs that accompanies such compartmentalization arises early in protein synthesis, when ribosomes engaged in the translation of mRNAs encoding signal-sequence-bearing proteins are targeted to the ER. In this report, we use multiple cell fractionation protocols, in combination with cDNA microarray, nuclease protection, and Northern blot analyses, to assess the distribution of mRNAs between free and ER-bound ribosomes. We find a broad representation of mRNAs encoding soluble proteins in the ER fraction, with a subset of such mRNAs displaying substantial ER partitioning. In addition, we present evidence that membrane-bound ribosomes engage in the translation of mRNAs encoding soluble proteins. Single-cell in situ hybridization analysis of the subcellular distribution of mRNAs encoding ER-localized and soluble proteins identify two overall patterns of mRNA distribution in the cell-endoplasmic reticular and cytosolic. However, both partitioning patterns include a distinct perinuclear component. These results identify previously unappreciated roles for membrane-bound ribosomes in the subcellular compartmentalization of protein synthesis and indicate possible functions for the perinuclear membrane domain in mRNA sorting in the cell.

Duke Scholars

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Published In

RNA

DOI

ISSN

1355-8382

Publication Date

September 2003

Volume

9

Issue

9

Start / End Page

1123 / 1137

Location

United States

Related Subject Headings

  • Ribosomes
  • RNA, Messenger
  • Protein Biosynthesis
  • Oligonucleotide Array Sequence Analysis
  • Microscopy, Electron
  • Jurkat Cells
  • Humans
  • Endoplasmic Reticulum
  • Developmental Biology
  • DNA, Complementary
 

Citation

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Lerner, R. S., Seiser, R. M., Zheng, T., Lager, P. J., Reedy, M. C., Keene, J. D., & Nicchitta, C. V. (2003). Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes. RNA, 9(9), 1123–1137. https://doi.org/10.1261/rna.5610403
Lerner, Rachel S., Robert M. Seiser, Tianli Zheng, Patrick J. Lager, Mary C. Reedy, Jack D. Keene, and Christopher V. Nicchitta. “Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes.RNA 9, no. 9 (September 2003): 1123–37. https://doi.org/10.1261/rna.5610403.
Lerner RS, Seiser RM, Zheng T, Lager PJ, Reedy MC, Keene JD, et al. Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes. RNA. 2003 Sep;9(9):1123–37.
Lerner, Rachel S., et al. “Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes.RNA, vol. 9, no. 9, Sept. 2003, pp. 1123–37. Pubmed, doi:10.1261/rna.5610403.
Lerner RS, Seiser RM, Zheng T, Lager PJ, Reedy MC, Keene JD, Nicchitta CV. Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes. RNA. 2003 Sep;9(9):1123–1137.

Published In

RNA

DOI

ISSN

1355-8382

Publication Date

September 2003

Volume

9

Issue

9

Start / End Page

1123 / 1137

Location

United States

Related Subject Headings

  • Ribosomes
  • RNA, Messenger
  • Protein Biosynthesis
  • Oligonucleotide Array Sequence Analysis
  • Microscopy, Electron
  • Jurkat Cells
  • Humans
  • Endoplasmic Reticulum
  • Developmental Biology
  • DNA, Complementary