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Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation.

Publication ,  Journal Article
Stephens, SB; Dodd, RD; Brewer, JW; Lager, PJ; Keene, JD; Nicchitta, CV
Published in: Mol Biol Cell
December 2005

In eukaryotic cells, protein synthesis is compartmentalized; mRNAs encoding secretory/membrane proteins are translated on endoplasmic reticulum (ER)-bound ribosomes, whereas mRNAs encoding cytosolic proteins are translated on free ribosomes. mRNA partitioning between the two compartments occurs via positive selection: free ribosomes engaged in the translation of signal sequence-encoding mRNAs are trafficked from the cytosol to the ER. After translation termination, ER-bound ribosomes are thought to dissociate, thereby completing a cycle of mRNA partitioning. At present, the physiological basis for termination-coupled ribosome release is unknown. To gain insight into this process, we examined ribosome and mRNA partitioning during the unfolded protein response, key elements of which include suppression of the initiation stage of protein synthesis and polyribosome breakdown. We report that unfolded protein response (UPR)-elicited polyribosome breakdown resulted in the continued association, rather than release, of ER-bound ribosomes. Under these conditions, mRNA translation in the cytosol was suppressed, whereas mRNA translation on the ER was sustained. Furthermore, mRNAs encoding key soluble stress proteins (XBP-1 and ATF-4) were translated primarily on ER-bound ribosomes. These studies demonstrate that ribosome release from the ER is termination independent and identify new and unexpected roles for the ER compartment in the translational response to induction of the unfolded protein response.

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Published In

Mol Biol Cell

DOI

ISSN

1059-1524

Publication Date

December 2005

Volume

16

Issue

12

Start / End Page

5819 / 5831

Location

United States

Related Subject Headings

  • Ultracentrifugation
  • Ribosomes
  • RNA, Messenger
  • Protein Biosynthesis
  • Plasmacytoma
  • Mice
  • Methionine
  • Gene Expression Regulation
  • Endoplasmic Reticulum
  • Developmental Biology
 

Citation

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Stephens, S. B., Dodd, R. D., Brewer, J. W., Lager, P. J., Keene, J. D., & Nicchitta, C. V. (2005). Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation. Mol Biol Cell, 16(12), 5819–5831. https://doi.org/10.1091/mbc.e05-07-0685
Stephens, Samuel B., Rebecca D. Dodd, Joseph W. Brewer, Patrick J. Lager, Jack D. Keene, and Christopher V. Nicchitta. “Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation.Mol Biol Cell 16, no. 12 (December 2005): 5819–31. https://doi.org/10.1091/mbc.e05-07-0685.
Stephens SB, Dodd RD, Brewer JW, Lager PJ, Keene JD, Nicchitta CV. Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation. Mol Biol Cell. 2005 Dec;16(12):5819–31.
Stephens, Samuel B., et al. “Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation.Mol Biol Cell, vol. 16, no. 12, Dec. 2005, pp. 5819–31. Pubmed, doi:10.1091/mbc.e05-07-0685.
Stephens SB, Dodd RD, Brewer JW, Lager PJ, Keene JD, Nicchitta CV. Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation. Mol Biol Cell. 2005 Dec;16(12):5819–5831.

Published In

Mol Biol Cell

DOI

ISSN

1059-1524

Publication Date

December 2005

Volume

16

Issue

12

Start / End Page

5819 / 5831

Location

United States

Related Subject Headings

  • Ultracentrifugation
  • Ribosomes
  • RNA, Messenger
  • Protein Biosynthesis
  • Plasmacytoma
  • Mice
  • Methionine
  • Gene Expression Regulation
  • Endoplasmic Reticulum
  • Developmental Biology