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The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1.

Publication ,  Journal Article
Crenshaw, DG; Yang, J; Means, AR; Kornbluth, S
Published in: EMBO J
August 10, 1998

The cis/trans peptidyl-prolyl isomerase, Pin1, is a regulator of mitosis that is well conserved from yeast to man. Here we demonstrate that depletion of Pin1-binding proteins from Xenopus egg extracts results in hyperphosphorylation and inactivation of the key mitotic regulator, Cdc2/cyclin B. We show biochemically that this phenotype is a consequence of Pin1 interaction with critical upstream regulators of Cdc2/cyclin B, including the Cdc2-directed phosphatase, Cdc25, and its known regulator, Plx1. Although Pin1 could interact with Plx1 during interphase and mitosis, only the phosphorylated, mitotically active form of Cdc25 was able to bind Pin1, an event we have recapitulated using in vitro phosphorylated Cdc25. Taken together, these data suggest that Pin1 may modulate cell cycle control through interaction with Cdc25 and its activator, Plx1.

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Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

August 10, 1998

Volume

17

Issue

5

Start / End Page

1315 / 1327

Location

England

Related Subject Headings

  • cdc25 Phosphatases
  • Xenopus Proteins
  • Xenopus
  • Saccharomyces cerevisiae Proteins
  • Recombinant Fusion Proteins
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Protein Binding
  • Phosphorylation
  • Phosphoprotein Phosphatases
 

Citation

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Crenshaw, D. G., Yang, J., Means, A. R., & Kornbluth, S. (1998). The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1. EMBO J, 17(5), 1315–1327. https://doi.org/10.1093/emboj/17.5.1315
Crenshaw, D. G., J. Yang, A. R. Means, and S. Kornbluth. “The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1.EMBO J 17, no. 5 (August 10, 1998): 1315–27. https://doi.org/10.1093/emboj/17.5.1315.
Crenshaw DG, Yang J, Means AR, Kornbluth S. The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1. EMBO J. 1998 Aug 10;17(5):1315–27.
Crenshaw, D. G., et al. “The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1.EMBO J, vol. 17, no. 5, Aug. 1998, pp. 1315–27. Pubmed, doi:10.1093/emboj/17.5.1315.
Crenshaw DG, Yang J, Means AR, Kornbluth S. The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1. EMBO J. 1998 Aug 10;17(5):1315–1327.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

August 10, 1998

Volume

17

Issue

5

Start / End Page

1315 / 1327

Location

England

Related Subject Headings

  • cdc25 Phosphatases
  • Xenopus Proteins
  • Xenopus
  • Saccharomyces cerevisiae Proteins
  • Recombinant Fusion Proteins
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Protein Binding
  • Phosphorylation
  • Phosphoprotein Phosphatases