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High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro.

Publication ,  Journal Article
Liu, Y; Kon, T; Li, C; Zhong, P
Published in: J Acoust Soc Am
November 2005

Cultured human cervical cancer (HeLa) and rat mammary carcinoma (R3230Ac) cells were transfected with vectors encoding green fluorescent protein (GFP) under the control of hsp70B promoter. Aliquots of 10-microl transfected cells (5 x 10(7) cells/ml) were placed in 0.2-ml thin-wall polymerase chain reaction tubes and exposed to 1.1-MHz high intensity focused ultrasound (HIFU) at a peak negative pressure P- = 2.68 MPa. By adjusting the duty cycle of the HIFU transducer, the cell suspensions were heated to a peak temperature from 50 to 70 degrees C in 1-10 s. Exposure dependent cell viability and gene activation were evaluated. For a 5-s HIFU exposure, cell viability dropped from 95% at 50 degrees C to 13% at 70 degrees C. Concomitantly, gene activation in sublethally injured tumor cells increased from 4% at 50 degrees C to 41% at 70 degrees C. A similar trend was observed at 60 degrees C peak temperature as the exposure time increased from 1 to 5 s. Further increase of exposure duration to 10 s led to significantly reduced cell viability and lower overall gene activation in exposed cells. Altogether, maximum HIFU-induced gene activation was achieved at 60 degrees C in 5 s. Under these experimental conditions, HIFU-induced gene activation was found to be produced primarily by thermal rather than mechanical stresses.

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Published In

J Acoust Soc Am

DOI

ISSN

0001-4966

Publication Date

November 2005

Volume

118

Issue

5

Start / End Page

3328 / 3336

Location

United States

Related Subject Headings

  • Ultrasonography
  • Ultrasonic Therapy
  • Transcriptional Activation
  • Stress, Mechanical
  • Neoplasms
  • In Vitro Techniques
  • Humans
  • Hot Temperature
  • Hela Cells
  • Heat-Shock Response
 

Citation

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Liu, Y., Kon, T., Li, C., & Zhong, P. (2005). High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro. J Acoust Soc Am, 118(5), 3328–3336. https://doi.org/10.1121/1.2041247
Liu, Yunbo, Takashi Kon, Chuanyuan Li, and Pei Zhong. “High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro.J Acoust Soc Am 118, no. 5 (November 2005): 3328–36. https://doi.org/10.1121/1.2041247.
Liu Y, Kon T, Li C, Zhong P. High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro. J Acoust Soc Am. 2005 Nov;118(5):3328–36.
Liu, Yunbo, et al. “High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro.J Acoust Soc Am, vol. 118, no. 5, Nov. 2005, pp. 3328–36. Pubmed, doi:10.1121/1.2041247.
Liu Y, Kon T, Li C, Zhong P. High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro. J Acoust Soc Am. 2005 Nov;118(5):3328–3336.

Published In

J Acoust Soc Am

DOI

ISSN

0001-4966

Publication Date

November 2005

Volume

118

Issue

5

Start / End Page

3328 / 3336

Location

United States

Related Subject Headings

  • Ultrasonography
  • Ultrasonic Therapy
  • Transcriptional Activation
  • Stress, Mechanical
  • Neoplasms
  • In Vitro Techniques
  • Humans
  • Hot Temperature
  • Hela Cells
  • Heat-Shock Response