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Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography.

Publication ,  Journal Article
Oldham, M; Sakhalkar, H; Oliver, T; Allan Johnson, G; Dewhirst, M
Published in: J Biomed Opt
2008

Optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) are new techniques that enable unprecedented high-resolution 3-D multimodal imaging of tissue structure and function. Applications include imaging macroscopic gene expression and microvasculature structure in unsectioned biological specimens up to 8 cm(3). A key requisite for these imaging techniques is effective sample preparation including optical clearing, which enables light transport through the sample while preserving the signal (either light absorbing stain or fluorescent proteins) in representative form. We review recent developments in optical-CT and optical-ECT, and compatible "fluorescence-friendly" optical clearing protocols.

Duke Scholars

Published In

J Biomed Opt

DOI

ISSN

1083-3668

Publication Date

2008

Volume

13

Issue

2

Start / End Page

021113

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Tomography, Optical
  • Subtraction Technique
  • Optics
  • Imaging, Three-Dimensional
  • Image Enhancement
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 1113 Opthalmology and Optometry
 

Citation

APA
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ICMJE
MLA
NLM
Oldham, M., Sakhalkar, H., Oliver, T., Allan Johnson, G., & Dewhirst, M. (2008). Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography. J Biomed Opt, 13(2), 021113. https://doi.org/10.1117/1.2907968
Oldham, Mark, Harshad Sakhalkar, Tim Oliver, G. Allan Johnson, and Mark Dewhirst. “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography.J Biomed Opt 13, no. 2 (2008): 021113. https://doi.org/10.1117/1.2907968.
Oldham M, Sakhalkar H, Oliver T, Allan Johnson G, Dewhirst M. Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography. J Biomed Opt. 2008;13(2):021113.
Oldham, Mark, et al. “Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography.J Biomed Opt, vol. 13, no. 2, 2008, p. 021113. Pubmed, doi:10.1117/1.2907968.
Oldham M, Sakhalkar H, Oliver T, Allan Johnson G, Dewhirst M. Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography. J Biomed Opt. 2008;13(2):021113.

Published In

J Biomed Opt

DOI

ISSN

1083-3668

Publication Date

2008

Volume

13

Issue

2

Start / End Page

021113

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Tomography, Optical
  • Subtraction Technique
  • Optics
  • Imaging, Three-Dimensional
  • Image Enhancement
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 1113 Opthalmology and Optometry