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A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities.

Publication ,  Journal Article
Snyder, PW; Lee, G; Marszalek, PE; Clark, RL; Toone, EJ
Published in: Proceedings of the National Academy of Sciences of the United States of America
February 2007

A cantilever device based on competitive binding of an immobilized receptor to immobilized and soluble ligand and capable of measuring solution-phase thermodynamic quantities is described. Through multiple binary queries, the device stochastically measures the probability of the formation of a bound complex between immobilized protein and immobilized ligand as a function of soluble ligand concentration. The resulting binding isotherm is described by a binding polynomial consisting of the activities of soluble and immobilized ligand and binding constants for the association of immobilized protein with free and immobilized ligand. Evaluation of the polynomial reveals an association constant for the formation of a complex between immobilized ligand and immobilized protein close to that for the formation of complex between soluble protein and soluble ligand. The methodology lays the foundation for construction of practical portable sensing devices.

Duke Scholars

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

February 2007

Volume

104

Issue

8

Start / End Page

2579 / 2584
 

Citation

APA
Chicago
ICMJE
MLA
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Snyder, P. W., Lee, G., Marszalek, P. E., Clark, R. L., & Toone, E. J. (2007). A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities. Proceedings of the National Academy of Sciences of the United States of America, 104(8), 2579–2584. https://doi.org/10.1073/pnas.0606604104
Snyder, Phillip W., Gwangrog Lee, Piotr E. Marszalek, Robert L. Clark, and Eric J. Toone. “A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities.Proceedings of the National Academy of Sciences of the United States of America 104, no. 8 (February 2007): 2579–84. https://doi.org/10.1073/pnas.0606604104.
Snyder PW, Lee G, Marszalek PE, Clark RL, Toone EJ. A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities. Proceedings of the National Academy of Sciences of the United States of America. 2007 Feb;104(8):2579–84.
Snyder, Phillip W., et al. “A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities.Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 8, Feb. 2007, pp. 2579–84. Epmc, doi:10.1073/pnas.0606604104.
Snyder PW, Lee G, Marszalek PE, Clark RL, Toone EJ. A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities. Proceedings of the National Academy of Sciences of the United States of America. 2007 Feb;104(8):2579–2584.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

February 2007

Volume

104

Issue

8

Start / End Page

2579 / 2584