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Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery.

Publication ,  Journal Article
Wang, Y; Wang, H; Li, C-Y; Yuan, F
Published in: Mol Cancer Ther
February 2006

Recent studies have shown that up to 90% of viral vectors could disseminate to normal organs following intratumoral infusion. The amount of dissemination might be dependent on the infusion conditions. Therefore, we investigated the effects of infusion rate, volume, and dose on transgene expression in liver and tumor tissues after intratumoral infusion of an adenoviral vector encoding luciferase. Luciferase expression was determined through bioluminescence intensity measurement. We observed that the luciferase expression in the liver was independent of the infusion rate but increased with the infusion dose, whereas the luciferase expression in the tumor was a bell-shaped function of the infusion rate. The latter observation was consistent with the distribution pattern of Evans blue-labeled albumin after its solution was infused into tumors at the same infusion rates. We also observed that the infusion volume could affect luciferase expression in the tumor but not in the liver. These observations implied that virus dissemination was determined mainly by the infusion dose, whereas the amount of transgene expression in the tumor depended on the distribution volume of viral vectors in the tumor as well as the infusion dose.

Duke Scholars

Published In

Mol Cancer Ther

DOI

ISSN

1535-7163

Publication Date

February 2006

Volume

5

Issue

2

Start / End Page

362 / 366

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Mice, Inbred BALB C
  • Mice
  • Luciferases
  • Liver
  • Genetic Vectors
  • Genetic Therapy
  • Genes, Reporter
  • Gene Transfer Techniques
  • Female
 

Citation

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Wang, Y., Wang, H., Li, C.-Y., & Yuan, F. (2006). Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery. Mol Cancer Ther, 5(2), 362–366. https://doi.org/10.1158/1535-7163.MCT-05-0266
Wang, Yong, He Wang, Chuan-Yuan Li, and Fan Yuan. “Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery.Mol Cancer Ther 5, no. 2 (February 2006): 362–66. https://doi.org/10.1158/1535-7163.MCT-05-0266.
Wang Y, Wang H, Li C-Y, Yuan F. Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery. Mol Cancer Ther. 2006 Feb;5(2):362–6.
Wang, Yong, et al. “Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery.Mol Cancer Ther, vol. 5, no. 2, Feb. 2006, pp. 362–66. Pubmed, doi:10.1158/1535-7163.MCT-05-0266.
Wang Y, Wang H, Li C-Y, Yuan F. Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery. Mol Cancer Ther. 2006 Feb;5(2):362–366.

Published In

Mol Cancer Ther

DOI

ISSN

1535-7163

Publication Date

February 2006

Volume

5

Issue

2

Start / End Page

362 / 366

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Mice, Inbred BALB C
  • Mice
  • Luciferases
  • Liver
  • Genetic Vectors
  • Genetic Therapy
  • Genes, Reporter
  • Gene Transfer Techniques
  • Female