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Air bubble-associated endothelial trauma in descemet stripping automated endothelial keratoplasty.

Publication ,  Journal Article
Hong, A; Caldwell, MC; Kuo, AN; Afshari, NA
Published in: Am J Ophthalmol
August 2009

PURPOSE: To evaluate endothelial cell trauma by anterior chamber (AC) air bubbles in Descemet stripping automated endothelial keratoplasty (DSAEK). DESIGN: Laboratory investigation. METHODS: Twelve human donor corneas (6 pairs) were sectioned using an automated microkeratome system (Moria ALTK System, Antony, France). One cornea of each pair was mounted on a Moria artificial AC, and an air bubble was injected to fill 40% of the AC. The apparatus was rotated 180 degrees for a total of 50 times to simulate air bubble trauma. The fellow corneas were used as controls. Each endothelial graft was stained with 0.25% Trypan blue for 90 seconds followed by 0.2% alizarin red for 2 minutes, and digital photomicrographs were obtained. Abnormally staining areas indicative of graft injury were removed digitally from the total graft area. The proportion of uninjured corneal endothelium was calculated, and differences were analyzed. RESULTS: In this ex vivo model of air bubble trauma, the proportion of viable graft endothelium after air bubble injury was 79.8 +/- 0.04% (n = 6). The proportion of viable endothelium in the control group was 89.9 +/- 0.02% (n = 6). The statistically significant mean difference of 10.1% (P = .03) is indicative of greater endothelial injury after air bubble trauma. CONCLUSIONS: Using this model, a moderate but significant amount of endothelial cell damage was associated with air bubble trauma compared with the control group. Air bubble trauma may account partially for the loss of endothelial cell density after DSAEK surgery and may impact graft survival.

Duke Scholars

Published In

Am J Ophthalmol

DOI

EISSN

1879-1891

Publication Date

August 2009

Volume

148

Issue

2

Start / End Page

256 / 259

Location

United States

Related Subject Headings

  • Tissue Donors
  • Staining and Labeling
  • Ophthalmology & Optometry
  • Humans
  • Graft Survival
  • Eye Injuries
  • Endothelium, Corneal
  • Descemet Membrane
  • Corneal Transplantation
  • Cell Survival
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hong, A., Caldwell, M. C., Kuo, A. N., & Afshari, N. A. (2009). Air bubble-associated endothelial trauma in descemet stripping automated endothelial keratoplasty. Am J Ophthalmol, 148(2), 256–259. https://doi.org/10.1016/j.ajo.2009.03.003
Hong, Anna, Matthew C. Caldwell, Anthony N. Kuo, and Natalie A. Afshari. “Air bubble-associated endothelial trauma in descemet stripping automated endothelial keratoplasty.Am J Ophthalmol 148, no. 2 (August 2009): 256–59. https://doi.org/10.1016/j.ajo.2009.03.003.
Hong A, Caldwell MC, Kuo AN, Afshari NA. Air bubble-associated endothelial trauma in descemet stripping automated endothelial keratoplasty. Am J Ophthalmol. 2009 Aug;148(2):256–9.
Hong, Anna, et al. “Air bubble-associated endothelial trauma in descemet stripping automated endothelial keratoplasty.Am J Ophthalmol, vol. 148, no. 2, Aug. 2009, pp. 256–59. Pubmed, doi:10.1016/j.ajo.2009.03.003.
Hong A, Caldwell MC, Kuo AN, Afshari NA. Air bubble-associated endothelial trauma in descemet stripping automated endothelial keratoplasty. Am J Ophthalmol. 2009 Aug;148(2):256–259.
Journal cover image

Published In

Am J Ophthalmol

DOI

EISSN

1879-1891

Publication Date

August 2009

Volume

148

Issue

2

Start / End Page

256 / 259

Location

United States

Related Subject Headings

  • Tissue Donors
  • Staining and Labeling
  • Ophthalmology & Optometry
  • Humans
  • Graft Survival
  • Eye Injuries
  • Endothelium, Corneal
  • Descemet Membrane
  • Corneal Transplantation
  • Cell Survival