Skip to main content

Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry.

Publication ,  Journal Article
Shaked, NT; Satterwhite, LL; Telen, MJ; Truskey, GA; Wax, A
Published in: J Biomed Opt
March 2011

We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical properties of RBCs. WFDI is a noncontact, label-free optical microscopy approach that can yield quantitative thickness profiles of RBCs and measurements of their membrane fluctuations at the nanometer scale reflecting their stiffness. We find that RBCs from individuals with SCA are significantly stiffer than those from a healthy control. Moreover, we show that the technique is sensitive enough to distinguish classes of RBCs in SCA, including sickle RBCs with apparently normal morphology, compared to the stiffer crescent-shaped sickle RBCs. We expect that this approach will be useful for diagnosis of SCA and for determining efficacy of therapeutic agents.

Duke Scholars

Published In

J Biomed Opt

DOI

EISSN

1560-2281

Publication Date

March 2011

Volume

16

Issue

3

Start / End Page

030506

Location

United States

Related Subject Headings

  • Reference Values
  • Optics
  • Optical Phenomena
  • Nanotechnology
  • Microscopy, Interference
  • Humans
  • Erythrocytes, Abnormal
  • Erythrocytes
  • Biomechanical Phenomena
  • Anemia, Sickle Cell
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shaked, N. T., Satterwhite, L. L., Telen, M. J., Truskey, G. A., & Wax, A. (2011). Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry. J Biomed Opt, 16(3), 030506. https://doi.org/10.1117/1.3556717
Shaked, Natan T., Lisa L. Satterwhite, Marilyn J. Telen, George A. Truskey, and Adam Wax. “Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry.J Biomed Opt 16, no. 3 (March 2011): 030506. https://doi.org/10.1117/1.3556717.
Shaked NT, Satterwhite LL, Telen MJ, Truskey GA, Wax A. Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry. J Biomed Opt. 2011 Mar;16(3):030506.
Shaked, Natan T., et al. “Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry.J Biomed Opt, vol. 16, no. 3, Mar. 2011, p. 030506. Pubmed, doi:10.1117/1.3556717.
Shaked NT, Satterwhite LL, Telen MJ, Truskey GA, Wax A. Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry. J Biomed Opt. 2011 Mar;16(3):030506.

Published In

J Biomed Opt

DOI

EISSN

1560-2281

Publication Date

March 2011

Volume

16

Issue

3

Start / End Page

030506

Location

United States

Related Subject Headings

  • Reference Values
  • Optics
  • Optical Phenomena
  • Nanotechnology
  • Microscopy, Interference
  • Humans
  • Erythrocytes, Abnormal
  • Erythrocytes
  • Biomechanical Phenomena
  • Anemia, Sickle Cell