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Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution.

Publication ,  Journal Article
Simpson, MJ; Wilson, JW; Phipps, MA; Robles, FE; Selim, MA; Warren, WS
Published in: J Invest Dermatol
July 2013

Pump-probe microscopy nondestructively differentiates eumelanin and pheomelanin and can be used to quantify melanin distributions in thin biopsy slices. Here we have extended that work for imaging eumelanin and pheomelanin distributions on a subcellular scale, allowing elucidation of characteristics of different cell types. The results show that melanin heterogeneity, previously found to be characteristic of melanomas, persists on the subcellular scale. We have also found spectral changes associated with melanin located in melanophages that could potentially differentiate invasive pigmented melanocytes from melanophages without immunohistochemical staining.

Duke Scholars

Published In

J Invest Dermatol

DOI

EISSN

1523-1747

Publication Date

July 2013

Volume

133

Issue

7

Start / End Page

1822 / 1826

Location

United States

Related Subject Headings

  • Spectroscopy, Near-Infrared
  • Skin Neoplasms
  • Microscopy, Fluorescence
  • Melanoma
  • Melanocytes
  • Melanins
  • Macrophages
  • Keratinocytes
  • Humans
  • Dermatology & Venereal Diseases
 

Citation

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Simpson, M. J., Wilson, J. W., Phipps, M. A., Robles, F. E., Selim, M. A., & Warren, W. S. (2013). Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution. J Invest Dermatol, 133(7), 1822–1826. https://doi.org/10.1038/jid.2013.37
Simpson, Mary Jane, Jesse W. Wilson, M Anthony Phipps, Francisco E. Robles, M Angelica Selim, and Warren S. Warren. “Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution.J Invest Dermatol 133, no. 7 (July 2013): 1822–26. https://doi.org/10.1038/jid.2013.37.
Simpson MJ, Wilson JW, Phipps MA, Robles FE, Selim MA, Warren WS. Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution. J Invest Dermatol. 2013 Jul;133(7):1822–6.
Simpson, Mary Jane, et al. “Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution.J Invest Dermatol, vol. 133, no. 7, July 2013, pp. 1822–26. Pubmed, doi:10.1038/jid.2013.37.
Simpson MJ, Wilson JW, Phipps MA, Robles FE, Selim MA, Warren WS. Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution. J Invest Dermatol. 2013 Jul;133(7):1822–1826.
Journal cover image

Published In

J Invest Dermatol

DOI

EISSN

1523-1747

Publication Date

July 2013

Volume

133

Issue

7

Start / End Page

1822 / 1826

Location

United States

Related Subject Headings

  • Spectroscopy, Near-Infrared
  • Skin Neoplasms
  • Microscopy, Fluorescence
  • Melanoma
  • Melanocytes
  • Melanins
  • Macrophages
  • Keratinocytes
  • Humans
  • Dermatology & Venereal Diseases