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Adapting phasor analysis for nonlinear pump-probe microscopy

Publication ,  Conference
Robles, FE; Wilson, JW; Fischer, MC; Warren, WS
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
May 28, 2013

Phasor analysis has become a powerful tool for examining signals in fluorescence life-time microscopy (FLIM), where the analysis provides a fast, robust and intuitive means of separating different fluorescent species and mixtures thereof. In this work we adapt this analysis for pump-probe microscopy, a method that provides molecular contrast of pigmented samples by probing their excited state dynamic properties. The bipolar nature of the pump-probe signals presents important differences in the resulting phasors compared to FLIM - here, we discuss these differences and describe the behavior of bipolar signals in phasor analysis. Results show that this method is indeed able to separate multiple molecular species of interests and allows facile assessment of pigment chemistry and its distribution in samples. © 2013 Copyright SPIE.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819493583

Publication Date

May 28, 2013

Volume

8589
 

Citation

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Robles, F. E., Wilson, J. W., Fischer, M. C., & Warren, W. S. (2013). Adapting phasor analysis for nonlinear pump-probe microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8589). https://doi.org/10.1117/12.2002600
Robles, F. E., J. W. Wilson, M. C. Fischer, and W. S. Warren. “Adapting phasor analysis for nonlinear pump-probe microscopy.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 8589, 2013. https://doi.org/10.1117/12.2002600.
Robles FE, Wilson JW, Fischer MC, Warren WS. Adapting phasor analysis for nonlinear pump-probe microscopy. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2013.
Robles, F. E., et al. “Adapting phasor analysis for nonlinear pump-probe microscopy.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8589, 2013. Scopus, doi:10.1117/12.2002600.
Robles FE, Wilson JW, Fischer MC, Warren WS. Adapting phasor analysis for nonlinear pump-probe microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2013.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819493583

Publication Date

May 28, 2013

Volume

8589