Barry W. Allen

Journal Article Protein J · October 2018 The isolation and characterization of 42 unique nonfunctional missense mutants in the bacterial cytosolic β-galactosidase and catechol 2,3-dioxygenase enzymes allowed us to examine some of the basic general trends regarding protein structure and function. ... Full text Link to item Cite

Engineered cardiac tissue patch maintains structural and electrical properties after epicardial implantation.

Journal Article Biomaterials · March 2018 Functional cardiac tissue engineering holds promise as a candidate therapy for myocardial infarction and heart failure. Generation of "strong-contracting and fast-conducting" cardiac tissue patches capable of electromechanical coupling with host myocardium ... Full text Link to item Cite

Cardiopatch platform enables maturation and scale-up of human pluripotent stem cell-derived engineered heart tissues.

Journal Article Nat Commun · November 28, 2017 Despite increased use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for drug development and disease modeling studies, methods to generate large, functional heart tissues for human therapy are lacking. Here we present a "Cardiop ... Full text Link to item Cite

Antiepileptic drugs prevent seizures in hyperbaric oxygen: A novel model of epileptiform activity.

Journal Article Brain Res · February 15, 2017 Breathing oxygen at sufficiently elevated pressures can trigger epileptiform seizures. Therefore, we tested the hypothesis that pre-treatment with FDA-approved antiepileptic drugs could prevent seizure onset in hyperoxia at 5 atmospheres absolute. We selec ... Full text Link to item Cite

Commentaries on Viewpoint: Anemia contributes to cardiovascular disease through reductions in nitric oxide.

Journal Article J Appl Physiol (1985) · February 1, 2017 Full text Link to item Cite

Effects of striatal nitric oxide production on regional cerebral blood flow and seizure development in rats exposed to extreme hyperoxia.

Journal Article J Appl Physiol (1985) · December 1, 2015 The endogenous vasodilator and signaling molecule nitric oxide has been implicated in cerebral hyperemia, sympathoexcitation, and seizures induced by hyperbaric oxygen (HBO2) at or above 3 atmospheres absolute (ATA). It is unknown whether these events in t ... Full text Open Access Link to item Cite

Baroreceptor afferents modulate brain excitation and influence susceptibility to toxic effects of hyperbaric oxygen.

Journal Article J Appl Physiol (1985) · September 1, 2014 Unexplained adjustments in baroreflex sensitivity occur in conjunction with exposures to potentially toxic levels of hyperbaric oxygen. To investigate this, we monitored central nervous system, autonomic and cardiovascular responses in conscious and anesth ... Full text Open Access Link to item Cite

Baroreflex-mediated cardiovascular responses to hyperbaric oxygen.

Journal Article J Appl Physiol (1985) · September 2013 The cardiovascular system responds to hyperbaric hyperoxia (HBO2) with vasoconstriction, hypertension, bradycardia, and reduced cardiac output (CO). We tested the hypothesis that these responses are linked by a common mechanism-activation of the arterial b ... Full text Link to item Cite

Brain oxygenation and CNS oxygen toxicity after infusion of perfluorocarbon emulsion.

Journal Article J Appl Physiol (1985) · July 2012 Intravenous perfluorocarbon (PFC) emulsions, administered with supplemental inspired O(2), are being evaluated for their ability to eliminate N(2) from blood and tissue prior to submarine escape, but these agents can increase the incidence of central nervo ... Full text Link to item Cite

Nitric oxide-mediated central sympathetic excitation promotes CNS and pulmonary O₂ toxicity.

Journal Article J Appl Physiol (1985) · June 2012 In hyperbaric oxygen (HBO(2)) at or above 3 atmospheres absolute (ATA), autonomic pathways link central nervous system (CNS) oxygen toxicity to pulmonary damage, possibly through a paradoxical and poorly characterized relationship between central nitric ox ... Full text Link to item Cite

Autonomic activation links CNS oxygen toxicity to acute cardiogenic pulmonary injury.

Journal Article Am J Physiol Lung Cell Mol Physiol · January 2011 Breathing hyperbaric oxygen (HBO₂), particularly at pressures above 3 atmospheres absolute, can cause acute pulmonary injury that is more severe if signs of central nervous system toxicity occur. This is consistent with the activation of an autonomic link ... Full text Link to item Cite

Hemoglobin, nitric oxide and molecular mechanisms of hypoxic vasodilation.

Journal Article Trends Mol Med · October 2009 The protected transport of nitric oxide (NO) by hemoglobin (Hb) links the metabolic activity of working tissue to the regulation of its local blood supply through hypoxic vasodilation. This physiologic mechanism is allosterically coupled to the O(2) satura ... Full text Link to item Cite

Phosphodiesterase-5 inhibitors oppose hyperoxic vasoconstriction and accelerate seizure development in rats exposed to hyperbaric oxygen.

Journal Article J Appl Physiol (1985) · April 2009 Oxygen is a potent cerebral vasoconstrictor, but excessive exposure to hyperbaric oxygen (HBO(2)) can reverse this vasoconstriction by stimulating brain nitric oxide (NO) production, which increases cerebral blood flow (CBF)-a predictor of O(2) convulsions ... Full text Link to item Cite

Two faces of nitric oxide: implications for cellular mechanisms of oxygen toxicity.

Journal Article J Appl Physiol (1985) · February 2009 Recent investigations have elucidated some of the diverse roles played by reactive oxygen and nitrogen species in events that lead to oxygen toxicity and defend against it. The focus of this review is on toxic and protective mechanisms in hyperoxia that ha ... Full text Link to item Cite

Contributions of nitric oxide synthase isoforms to pulmonary oxygen toxicity, local vs. mediated effects.

Journal Article Am J Physiol Lung Cell Mol Physiol · May 2008 Reactive species of oxygen and nitrogen have been collectively implicated in pulmonary oxygen toxicity, but the contributions of specific molecules are unknown. Therefore, we assessed the roles of several reactive species, particularly nitric oxide, in pul ... Full text Link to item Cite

Similar but not the same: normobaric and hyperbaric pulmonary oxygen toxicity, the role of nitric oxide.

Journal Article Am J Physiol Lung Cell Mol Physiol · July 2007 Pulmonary manifestations of oxygen toxicity were studied and quantified in rats breathing >98% O(2) at 1, 1.5, 2, 2.5, and 3 ATA to test our hypothesis that different patterns of pulmonary injury would emerge, reflecting a role for central nervous system ( ... Full text Link to item Cite

How do red blood cells cause hypoxic vasodilation? The SNO-hemoglobin paradigm.

Journal Article Am J Physiol Heart Circ Physiol · October 2006 One of the most intriguing areas of research in erythrocyte physiology is the interaction of hemoglobin with nitric oxide (NO). These two molecules independently fulfill diverse and complex physiological roles, while together they subtly modulate microvasc ... Full text Link to item Cite

A novel electrochemical sensor for nitric oxide using aligned RuO 2 nanowires on a Pt filament

Journal Article 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings · December 1, 2005 A novel electrochemical sensor was prepared for detecting nitric oxide (NO) in biological materials using aligned ruthenium oxide nanowires deposited on a Pt filament by chemical vapor deposition (CVD) in two steps. First, ruthenium carbonyl (Ru 3(CO) 12) ... Cite

Cerebral blood flow and brain oxygenation in rats breathing oxygen under pressure.

Journal Article J Cereb Blood Flow Metab · October 2005 Featured Publication Hyperbaric oxygen (HBO(2)) increases oxygen tension (PO(2)) in blood but reduces blood flow by means of O(2)-induced vasoconstriction. Here we report the first quantitative evaluation of these opposing effects on tissue PO(2) in brain, using anesthetized r ... Full text Link to item Cite

Electrochemical detection of nitric oxide in biological fluids.

Journal Article Methods Enzymol · 2005 Featured Publication The challenges that must be overcome in order to detect nitric oxide (NO) in biological fluids include its low physiological concentration (1-nM) and its short half-life (a few seconds or less). Electrochemistry is capable of making such measurements, if c ... Full text Link to item Cite

How do red blood cells dilate blood vessels?

Journal Article Circ Res · June 25, 2004 Featured Publication Link to item Cite

Electrochemical activation of electrodes for amperometric detection of nitric oxide.

Journal Article Nitric Oxide · June 2003 Featured Publication An open question in the literature of nitric oxide detection was investigated: does electrochemical activation account for the enhanced properties of certain presumed chemically-modified electrodes? Uniform electrodes of graphite, iridium, palladium, plati ... Full text Link to item Cite

Electrochemical detection of physiological nitric oxide: materials and methods.

Journal Article Methods Enzymol · 2002 Featured Publication Advances in the electroanalytical technology of NO detection make it possible to detect the release of robust concentrations of NO from living systems under pathological or pharmacological conditions. However, technical improvements should enable the const ... Full text Link to item Cite

Activation of NO electrodes

Journal Article FREE RADICAL BIOLOGY AND MEDICINE · January 1, 2001 Link to item Cite

Electrode materials for nitric oxide detection.

Journal Article Nitric Oxide · February 2000 Featured Publication Nitric oxide oxidation signals were compared for uniform test electrodes of platinum, iridium, palladium, rhodium, ruthenium, gold, graphite, and a nickel-porphyrin on graphite in deaerated phosphate-buffered saline (pH 7.0) at 35 degrees C. All tested mat ... Full text Link to item Cite

Enzymatically amplified voltammetric sensor for microliter sample volumes of salicylate.

Journal Article Anal Chem · June 1, 1995 Featured Publication A new voltammetric sensing strategy for salicylate employing two enzymes and applicable to microliter sample volumes is demonstrated. The method involves the use of the enzyme salicylate hydroxylase to convert salicylate to catechol, which is oxidized at a ... Full text Link to item Cite

Pathophysiology of pH and Ca2+ in bloodstream and brain.

Journal Article Can J Physiol Pharmacol · May 1987 Featured Publication The highlights of the literature and our work on tetany and hyperventilation are reviewed. Our studies concern the following: (1) the changes of [Ca2+] in circulating plasma caused by respiratory and "metabolic" acidosis and alkalosis; (2) critical plasma ... Full text Link to item Cite