Journal ArticleProtein J · October 2018
The isolation and characterization of 42 unique nonfunctional missense mutants in the bacterial cytosolic β-galactosidase and catechol 2,3-dioxygenase enzymes allowed us to examine some of the basic general trends regarding protein structure and function. ...
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Journal ArticleBiomaterials · March 2018
Functional cardiac tissue engineering holds promise as a candidate therapy for myocardial infarction and heart failure. Generation of "strong-contracting and fast-conducting" cardiac tissue patches capable of electromechanical coupling with host myocardium ...
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Journal ArticleNat Commun · November 28, 2017
Despite increased use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for drug development and disease modeling studies, methods to generate large, functional heart tissues for human therapy are lacking. Here we present a "Cardiop ...
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Journal ArticleBrain Res · February 15, 2017
Breathing oxygen at sufficiently elevated pressures can trigger epileptiform seizures. Therefore, we tested the hypothesis that pre-treatment with FDA-approved antiepileptic drugs could prevent seizure onset in hyperoxia at 5 atmospheres absolute. We selec ...
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Journal ArticleJ Appl Physiol (1985) · December 1, 2015
The endogenous vasodilator and signaling molecule nitric oxide has been implicated in cerebral hyperemia, sympathoexcitation, and seizures induced by hyperbaric oxygen (HBO2) at or above 3 atmospheres absolute (ATA). It is unknown whether these events in t ...
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Journal ArticleJ Appl Physiol (1985) · September 1, 2014
Unexplained adjustments in baroreflex sensitivity occur in conjunction with exposures to potentially toxic levels of hyperbaric oxygen. To investigate this, we monitored central nervous system, autonomic and cardiovascular responses in conscious and anesth ...
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Journal ArticleJ Appl Physiol (1985) · September 2013
The cardiovascular system responds to hyperbaric hyperoxia (HBO2) with vasoconstriction, hypertension, bradycardia, and reduced cardiac output (CO). We tested the hypothesis that these responses are linked by a common mechanism-activation of the arterial b ...
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Journal ArticleJ Appl Physiol (1985) · July 2012
Intravenous perfluorocarbon (PFC) emulsions, administered with supplemental inspired O(2), are being evaluated for their ability to eliminate N(2) from blood and tissue prior to submarine escape, but these agents can increase the incidence of central nervo ...
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Journal ArticleJ Appl Physiol (1985) · June 2012
In hyperbaric oxygen (HBO(2)) at or above 3 atmospheres absolute (ATA), autonomic pathways link central nervous system (CNS) oxygen toxicity to pulmonary damage, possibly through a paradoxical and poorly characterized relationship between central nitric ox ...
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Journal ArticleAm J Physiol Lung Cell Mol Physiol · January 2011
Breathing hyperbaric oxygen (HBO₂), particularly at pressures above 3 atmospheres absolute, can cause acute pulmonary injury that is more severe if signs of central nervous system toxicity occur. This is consistent with the activation of an autonomic link ...
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Journal ArticleTrends Mol Med · October 2009
The protected transport of nitric oxide (NO) by hemoglobin (Hb) links the metabolic activity of working tissue to the regulation of its local blood supply through hypoxic vasodilation. This physiologic mechanism is allosterically coupled to the O(2) satura ...
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Journal ArticleJ Appl Physiol (1985) · April 2009
Oxygen is a potent cerebral vasoconstrictor, but excessive exposure to hyperbaric oxygen (HBO(2)) can reverse this vasoconstriction by stimulating brain nitric oxide (NO) production, which increases cerebral blood flow (CBF)-a predictor of O(2) convulsions ...
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Journal ArticleJ Appl Physiol (1985) · February 2009
Recent investigations have elucidated some of the diverse roles played by reactive oxygen and nitrogen species in events that lead to oxygen toxicity and defend against it. The focus of this review is on toxic and protective mechanisms in hyperoxia that ha ...
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Journal ArticleAm J Physiol Lung Cell Mol Physiol · May 2008
Reactive species of oxygen and nitrogen have been collectively implicated in pulmonary oxygen toxicity, but the contributions of specific molecules are unknown. Therefore, we assessed the roles of several reactive species, particularly nitric oxide, in pul ...
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Journal ArticleAm J Physiol Lung Cell Mol Physiol · July 2007
Pulmonary manifestations of oxygen toxicity were studied and quantified in rats breathing >98% O(2) at 1, 1.5, 2, 2.5, and 3 ATA to test our hypothesis that different patterns of pulmonary injury would emerge, reflecting a role for central nervous system ( ...
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Journal ArticleAm J Physiol Heart Circ Physiol · October 2006
One of the most intriguing areas of research in erythrocyte physiology is the interaction of hemoglobin with nitric oxide (NO). These two molecules independently fulfill diverse and complex physiological roles, while together they subtly modulate microvasc ...
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Journal Article2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings · December 1, 2005
A novel electrochemical sensor was prepared for detecting nitric oxide (NO) in biological materials using aligned ruthenium oxide nanowires deposited on a Pt filament by chemical vapor deposition (CVD) in two steps. First, ruthenium carbonyl (Ru 3(CO) 12) ...
Cite
Journal ArticleJ Cereb Blood Flow Metab · October 2005
Featured Publication
Hyperbaric oxygen (HBO(2)) increases oxygen tension (PO(2)) in blood but reduces blood flow by means of O(2)-induced vasoconstriction. Here we report the first quantitative evaluation of these opposing effects on tissue PO(2) in brain, using anesthetized r ...
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Journal ArticleMethods Enzymol · 2005
Featured Publication
The challenges that must be overcome in order to detect nitric oxide (NO) in biological fluids include its low physiological concentration (1-nM) and its short half-life (a few seconds or less). Electrochemistry is capable of making such measurements, if c ...
Full textLink to itemCite
Journal ArticleProtein J · October 2018
The isolation and characterization of 42 unique nonfunctional missense mutants in the bacterial cytosolic β-galactosidase and catechol 2,3-dioxygenase enzymes allowed us to examine some of the basic general trends regarding protein structure and function. ...
Full textLink to itemCite
Journal ArticleBiomaterials · March 2018
Functional cardiac tissue engineering holds promise as a candidate therapy for myocardial infarction and heart failure. Generation of "strong-contracting and fast-conducting" cardiac tissue patches capable of electromechanical coupling with host myocardium ...
Full textLink to itemCite
Journal ArticleNat Commun · November 28, 2017
Despite increased use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for drug development and disease modeling studies, methods to generate large, functional heart tissues for human therapy are lacking. Here we present a "Cardiop ...
Full textLink to itemCite
Journal ArticleBrain Res · February 15, 2017
Breathing oxygen at sufficiently elevated pressures can trigger epileptiform seizures. Therefore, we tested the hypothesis that pre-treatment with FDA-approved antiepileptic drugs could prevent seizure onset in hyperoxia at 5 atmospheres absolute. We selec ...
Full textLink to itemCite
Journal ArticleJ Appl Physiol (1985) · December 1, 2015
The endogenous vasodilator and signaling molecule nitric oxide has been implicated in cerebral hyperemia, sympathoexcitation, and seizures induced by hyperbaric oxygen (HBO2) at or above 3 atmospheres absolute (ATA). It is unknown whether these events in t ...
Full textOpen AccessLink to itemCite
Journal ArticleJ Appl Physiol (1985) · September 1, 2014
Unexplained adjustments in baroreflex sensitivity occur in conjunction with exposures to potentially toxic levels of hyperbaric oxygen. To investigate this, we monitored central nervous system, autonomic and cardiovascular responses in conscious and anesth ...
Full textOpen AccessLink to itemCite
Journal ArticleJ Appl Physiol (1985) · September 2013
The cardiovascular system responds to hyperbaric hyperoxia (HBO2) with vasoconstriction, hypertension, bradycardia, and reduced cardiac output (CO). We tested the hypothesis that these responses are linked by a common mechanism-activation of the arterial b ...
Full textLink to itemCite
Journal ArticleJ Appl Physiol (1985) · July 2012
Intravenous perfluorocarbon (PFC) emulsions, administered with supplemental inspired O(2), are being evaluated for their ability to eliminate N(2) from blood and tissue prior to submarine escape, but these agents can increase the incidence of central nervo ...
Full textLink to itemCite
Journal ArticleJ Appl Physiol (1985) · June 2012
In hyperbaric oxygen (HBO(2)) at or above 3 atmospheres absolute (ATA), autonomic pathways link central nervous system (CNS) oxygen toxicity to pulmonary damage, possibly through a paradoxical and poorly characterized relationship between central nitric ox ...
Full textLink to itemCite
Journal ArticleAm J Physiol Lung Cell Mol Physiol · January 2011
Breathing hyperbaric oxygen (HBO₂), particularly at pressures above 3 atmospheres absolute, can cause acute pulmonary injury that is more severe if signs of central nervous system toxicity occur. This is consistent with the activation of an autonomic link ...
Full textLink to itemCite
Journal ArticleTrends Mol Med · October 2009
The protected transport of nitric oxide (NO) by hemoglobin (Hb) links the metabolic activity of working tissue to the regulation of its local blood supply through hypoxic vasodilation. This physiologic mechanism is allosterically coupled to the O(2) satura ...
Full textLink to itemCite
Journal ArticleJ Appl Physiol (1985) · April 2009
Oxygen is a potent cerebral vasoconstrictor, but excessive exposure to hyperbaric oxygen (HBO(2)) can reverse this vasoconstriction by stimulating brain nitric oxide (NO) production, which increases cerebral blood flow (CBF)-a predictor of O(2) convulsions ...
Full textLink to itemCite
Journal ArticleJ Appl Physiol (1985) · February 2009
Recent investigations have elucidated some of the diverse roles played by reactive oxygen and nitrogen species in events that lead to oxygen toxicity and defend against it. The focus of this review is on toxic and protective mechanisms in hyperoxia that ha ...
Full textLink to itemCite
Journal ArticleAm J Physiol Lung Cell Mol Physiol · May 2008
Reactive species of oxygen and nitrogen have been collectively implicated in pulmonary oxygen toxicity, but the contributions of specific molecules are unknown. Therefore, we assessed the roles of several reactive species, particularly nitric oxide, in pul ...
Full textLink to itemCite
Journal ArticleAm J Physiol Lung Cell Mol Physiol · July 2007
Pulmonary manifestations of oxygen toxicity were studied and quantified in rats breathing >98% O(2) at 1, 1.5, 2, 2.5, and 3 ATA to test our hypothesis that different patterns of pulmonary injury would emerge, reflecting a role for central nervous system ( ...
Full textLink to itemCite
Journal ArticleAm J Physiol Heart Circ Physiol · October 2006
One of the most intriguing areas of research in erythrocyte physiology is the interaction of hemoglobin with nitric oxide (NO). These two molecules independently fulfill diverse and complex physiological roles, while together they subtly modulate microvasc ...
Full textLink to itemCite
Journal Article2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings · December 1, 2005
A novel electrochemical sensor was prepared for detecting nitric oxide (NO) in biological materials using aligned ruthenium oxide nanowires deposited on a Pt filament by chemical vapor deposition (CVD) in two steps. First, ruthenium carbonyl (Ru 3(CO) 12) ...
Cite
Journal ArticleJ Cereb Blood Flow Metab · October 2005
Featured Publication
Hyperbaric oxygen (HBO(2)) increases oxygen tension (PO(2)) in blood but reduces blood flow by means of O(2)-induced vasoconstriction. Here we report the first quantitative evaluation of these opposing effects on tissue PO(2) in brain, using anesthetized r ...
Full textLink to itemCite
Journal ArticleMethods Enzymol · 2005
Featured Publication
The challenges that must be overcome in order to detect nitric oxide (NO) in biological fluids include its low physiological concentration (1-nM) and its short half-life (a few seconds or less). Electrochemistry is capable of making such measurements, if c ...
Full textLink to itemCite
Journal ArticleNitric Oxide · June 2003
Featured Publication
An open question in the literature of nitric oxide detection was investigated: does electrochemical activation account for the enhanced properties of certain presumed chemically-modified electrodes? Uniform electrodes of graphite, iridium, palladium, plati ...
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Journal ArticleMethods Enzymol · 2002
Featured Publication
Advances in the electroanalytical technology of NO detection make it possible to detect the release of robust concentrations of NO from living systems under pathological or pharmacological conditions. However, technical improvements should enable the const ...
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Journal ArticleNitric Oxide · February 2000
Featured Publication
Nitric oxide oxidation signals were compared for uniform test electrodes of platinum, iridium, palladium, rhodium, ruthenium, gold, graphite, and a nickel-porphyrin on graphite in deaerated phosphate-buffered saline (pH 7.0) at 35 degrees C. All tested mat ...
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Journal ArticleAnal Chem · June 1, 1995
Featured Publication
A new voltammetric sensing strategy for salicylate employing two enzymes and applicable to microliter sample volumes is demonstrated. The method involves the use of the enzyme salicylate hydroxylase to convert salicylate to catechol, which is oxidized at a ...
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Journal ArticleCan J Physiol Pharmacol · May 1987
Featured Publication
The highlights of the literature and our work on tetany and hyperventilation are reviewed. Our studies concern the following: (1) the changes of [Ca2+] in circulating plasma caused by respiratory and "metabolic" acidosis and alkalosis; (2) critical plasma ...
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