Brian Edward Coggins

Chapter · January 1, 2017 CLEAN is an algorithm for the suppression of artifacts from nonuniform sampling, originally developed in the field of radioastronomy in the 1960s and 1970s. Recognizing similarities between the problem of NMR NUS and astronomical data collection, several N ... Full text Cite

Backprojection and Related Methods

Chapter · January 1, 2017 The sampling of the NMR time domain along radial spokes allows one to obtain projections of an NMR spectrum at various angles. These projections encode information about the positions, intensities, and lineshapes of the peaks in the spectrum, and this info ... Full text Cite

Unbiased measurements of reconstruction fidelity of sparsely sampled magnetic resonance spectra.

Journal Article Nat Commun · July 27, 2016 The application of sparse-sampling techniques to NMR data acquisition would benefit from reliable quality measurements for reconstructed spectra. We introduce a pair of noise-normalized measurements, and , for differentiating inadequate modelling from over ... Full text Open Access Link to item Cite

3D TOCSY-HSQC NMR for Metabolic Flux Analysis Using Non-Uniform Sampling.

Journal Article Anal Chem · March 1, 2016 (13)C-Metabolic Flux Analysis ((13)C-MFA) is rapidly being recognized as the authoritative method for determining fluxes through metabolic networks. Site-specific (13)C enrichment information obtained using NMR spectroscopy is a valuable input for (13)C-MF ... Full text Link to item Cite

Rapid protein global fold determination using ultrasparse sampling, high-dynamic range artifact suppression, and time-shared NOESY.

Journal Article J Am Chem Soc · November 14, 2012 In structural studies of large proteins by NMR, global fold determination plays an increasingly important role in providing a first look at a target's topology and reducing assignment ambiguity in NOESY spectra of fully protonated samples. In this work, we ... Full text Link to item Cite

Radial sampling for fast NMR: Concepts and practices over three decades.

Journal Article Prog Nucl Magn Reson Spectrosc · November 2010 Full text Link to item Cite

Fast acquisition of high resolution 4-D amide-amide NOESY with diagonal suppression, sparse sampling and FFT-CLEAN.

Journal Article J Magn Reson · May 2010 Amide-amide NOESY provides important distance constraints for calculating global folds of large proteins, especially integral membrane proteins with beta-barrel folds. Here, we describe a diagonal-suppressed 4-D NH-NH TROSY-NOESY-TROSY (ds-TNT) experiment ... Full text Link to item Cite

High resolution 4-D spectroscopy with sparse concentric shell sampling and FFT-CLEAN.

Journal Article J Biomol NMR · December 2008 Recent efforts to reduce the measurement time for multidimensional NMR experiments have fostered the development of a variety of new procedures for sampling and data processing. We recently described concentric ring sampling for 3-D NMR experiments, which ... Full text Link to item Cite

Sampling of the NMR time domain along concentric rings.

Journal Article J Magn Reson · February 2007 We present a novel approach to sampling the NMR time domain, whereby the sampling points are aligned on concentric rings, which we term concentric ring sampling (CRS). Radial sampling constitutes a special case of CRS where each ring has the same number of ... Full text Link to item Cite

Polar Fourier transforms of radially sampled NMR data.

Journal Article J Magn Reson · September 2006 Radial sampling of the NMR time domain has recently been introduced to speed up data collection significantly. Here, we show that radially sampled data can be processed directly using Fourier transforms in polar coordinates. We present a comprehensive theo ... Full text Link to item Cite

PR-CALC: a program for the reconstruction of NMR spectra from projections.

Journal Article J Biomol NMR · March 2006 Projection-reconstruction NMR (PR-NMR) has attracted growing attention as a method for collecting multidimensional NMR data rapidly. The PR-NMR procedure involves measuring lower-dimensional projections of a higher-dimensional spectrum, which are then used ... Full text Link to item Cite

Filtered backprojection for the reconstruction of a high-resolution (4,2)D CH3-NH NOESY spectrum on a 29 kDa protein.

Journal Article J Am Chem Soc · August 24, 2005 Projection-reconstruction (PR) NMR enables rapid collection of multidimensional NMR data. NOESY represents a particularly difficult challenge for currently existing reconstruction algorithms, as it requires the quantitative reconstruction of an unknown num ... Full text Link to item Cite

Rapid assignment of protein side chain resonances using projection-reconstruction of (4,3)D HC(CCO)NH and intra-HC(C)NH experiments.

Journal Article J Magn Reson · July 2005 The reconstruction of higher-dimensional NMR spectra from projections can provide significant savings in instrument time. Here, we demonstrate its application to the (4,3)D HC(CCO)NH and intra-HC(C)NH experiments. The latter experiment contains a novel int ... Full text Link to item Cite

(4,2)D Projection--reconstruction experiments for protein backbone assignment: application to human carbonic anhydrase II and calbindin D(28K).

Journal Article J Am Chem Soc · June 22, 2005 Projection-reconstruction NMR experiments have been shown to significantly reduce the acquisition time required to obtain protein backbone assignment data. To date, this concept has only been applied to smaller (15)N/(13)C-labeled proteins. Here, we show t ... Full text Link to item Cite

Refined solution structure of the LpxC-TU-514 complex and pKa analysis of an active site histidine: insights into the mechanism and inhibitor design.

Journal Article Biochemistry · February 1, 2005 Lipopolysaccharide, the major constituent of the outer monolayer of the outer membrane of Gram-negative bacteria, is anchored into the membrane through the hydrophobic moiety lipid A, a hexaacylated disaccharide. The zinc-dependent metalloamidase UDP-3-O-a ... Full text Link to item Cite

Generalized reconstruction of n-D NMR spectra from multiple projections: application to the 5-D HACACONH spectrum of protein G B1 domain.

Journal Article J Am Chem Soc · February 4, 2004 Reconstructing multidimensional NMR spectra from 2-D projections significantly reduces the time needed for data collection over conventional methodology. Here, we provide a generalization of the projection-reconstruction process to spectra of arbitrary dim ... Full text Link to item Cite

Assignment of the 1H, 13C and 15N resonances of the LpxC deacetylase from Aquifex aeolicus in complex with the substrate-analog inhibitor TU-514.

Journal Article J Biomol NMR · February 2004 Full text Link to item Cite

Structure of the LpxC deacetylase with a bound substrate-analog inhibitor.

Journal Article Nat Struct Biol · August 2003 The zinc-dependent UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) catalyzes the first committed step in the biosynthesis of lipid A, the hydrophobic anchor of lipopolysaccharide (LPS) that constitutes the outermost monolayer of Gram-negative bacteria. ... Full text Link to item Cite

PACES: Protein sequential assignment by computer-assisted exhaustive search.

Journal Article J Biomol NMR · June 2003 A crucial step in determining solution structures of proteins using nuclear magnetic resonance (NMR) spectroscopy is the process of sequential assignment, which correlates backbone resonances to corresponding residues in the primary sequence of a protein, ... Full text Link to item Cite