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Friederike Luking Jayes

Assistant Professor in Obstetrics and Gynecology
Obstetrics and Gynecology, Reproductive Sciences

Selected Publications


Using rheology to quantify the effects of localized collagenase treatments on uterine fibroid digestion.

Journal Article Acta Biomater · October 15, 2021 Uterine fibroids are stiff, benign tumors containing excessive, disordered collagens that occur in 70-80% of women before age 50 and cause bleeding and pain. Collagenase Clostridium histolyticum (CCH) is a bacterial enzyme capable of digesting the collagen ... Full text Link to item Cite

A Phase I Clinical Trial to Assess Safety and Tolerability of Injectable Collagenase in Women with Symptomatic Uterine Fibroids.

Journal Article Reprod Sci · September 2021 Uterine fibroids feature excessive deposition of types I and III collagen. Previous ex vivo studies showed an FDA-approved collagenase (EN3835)-digested types I and III collagen fibers in fibroid tissues; however, collagenase had not been evaluated in vivo ... Full text Link to item Cite

Extracellular matrix and Hippo signaling as therapeutic targets of antifibrotic compounds for uterine fibroids.

Journal Article Clin Transl Med · July 2021 BACKGROUND: Uterine fibroids are highly prevalent, collagen-rich, mechanically stiff, fibrotic tumors for which new therapeutic options are needed. Increased extracellular matrix (ECM) stiffness activates mechanical signaling and Hippo/YAP promoting fibroi ... Full text Link to item Cite

Evaluation of Host Immune Cellular and Extracellular Matrix Responses to Prolapse Mesh With and Without Tension in a Rat Model.

Journal Article Female Pelvic Med Reconstr Surg · February 1, 2021 OBJECTIVES: We sought to determine whether vaginal host immune cellular and extracellular matrix responses are altered in a rat sacrocolpopexy model when lightweight polypropylene mesh is attached on tension versus without tension. METHODS: We performed hy ... Full text Link to item Cite

Polycarbonate Urethane Mesh: A New Material for Pelvic Reconstruction.

Journal Article Female Pelvic Med Reconstr Surg · February 1, 2021 OBJECTIVE: Polycarbonate urethane (PCU) is a new biomaterial, and its mechanical properties can be tailored to match that of vaginal tissue. We aimed to determine whether vaginal host immune and extracellular matrix responses differ after PCU versus lightw ... Full text Link to item Cite

Summary of the Proceedings of the Basic Science of Uterine Fibroids Meeting: New Developments February 28, 2020.

Journal Article F S Sci · February 2021 Scientists from multiple basic disciplines and an international group of physician-scientists from the field of obstetrics and gynecology presented recent studies and discussed new and evolving theories of uterine fibroid etiology, growth and development a ... Full text Link to item Cite

Evidence of biomechanical and collagen heterogeneity in uterine fibroids.

Journal Article PLoS One · 2019 OBJECTIVE: Uterine fibroids (leiomyomas) are common benign tumors of the myometrium but their molecular pathobiology remains elusive. These stiff and often large tumors contain abundant extracellular matrix (ECM), including large amounts of collagen, and c ... Full text Link to item Cite

Biochemical Pathways and Myometrial Cell Differentiation Leading to Nodule Formation Containing Collagen and Fibronectin.

Journal Article Curr Protein Pept Sci · 2017 Utilizing both primary myometrial cells and a myometrial cell line, we show here that myometrial cells undergo transition to a myofibroblast-like phenotype after a biological insult of 72 hours serum starvation and serum add-back (SB: 1% to 10% FBS). We al ... Full text Link to item Cite

Loss of stiffness in collagen-rich uterine fibroids after digestion with purified collagenase Clostridium histolyticum.

Journal Article Am J Obstet Gynecol · November 2016 BACKGROUND: Uterine fibroids are a significant health problem. These common benign tumors occur in 70-80% of women before age 50 years and often cause bleeding and pain and can interfere considerably with daily life. Current treatment options are limited. ... Full text Link to item Cite

Inflammatory Response of Human Gestational Membranes to Ureaplasma parvum Using a Novel Dual-Chamber Tissue Explant System.

Journal Article Biol Reprod · May 2016 Preterm premature rupture of membranes (PPROM) is often associated with intra-amniotic inflammation and infection. Current understanding of the pathogenesis of PPROM includes activation of pro-inflammatory cytokines and proteolytic enzymes leading to compr ... Full text Link to item Cite

341: Novel ex vivo system to evaluate inflammation of gestational membranes in response to U. parvum

Conference American Journal of Obstetrics and Gynecology · January 2016 Full text Cite

Injectable Clostridium histolyticum collagenase as a potential treatment for uterine fibroids.

Journal Article Reprod Sci · December 2014 Purified Clostridium histolyticum collagenase (CHC), an Food and Drug Administration-approved drug that does not affect nerves or blood vessels, was assessed as a potential treatment for fibroids in this proof-of-principle study. Fibroids (1-4 cm, capsules ... Full text Link to item Cite

The naturally occurring luteinizing hormone surge is diminished in mice lacking estrogen receptor Beta in the ovary.

Journal Article Biol Reprod · February 2014 Female ESR2-null mice (betaERKO) display defects in ovarian function and are subfertile. Follicular maturation is impaired and explains smaller litters, but betaERKO also produce fewer litters, which may be partially due to inadequate ovulatory signals. To ... Full text Link to item Cite

The extracellular matrix contributes to mechanotransduction in uterine fibroids.

Journal Article Obstet Gynecol Int · 2014 The role of the extracellular matrix (ECM) and mechanotransduction as an important signaling factor in the human uterus is just beginning to be appreciated. The ECM is not only the substance that surrounds cells, but ECM stiffness will either compress cell ... Full text Open Access Link to item Cite

Organotypic 3D-culture of uterine fibroid cells

Conference GLYCOBIOLOGY · November 1, 2012 Link to item Cite

Using rheology to quantify the effects of localized collagenase treatments on uterine fibroid digestion.

Journal Article Acta Biomater · October 15, 2021 Uterine fibroids are stiff, benign tumors containing excessive, disordered collagens that occur in 70-80% of women before age 50 and cause bleeding and pain. Collagenase Clostridium histolyticum (CCH) is a bacterial enzyme capable of digesting the collagen ... Full text Link to item Cite

A Phase I Clinical Trial to Assess Safety and Tolerability of Injectable Collagenase in Women with Symptomatic Uterine Fibroids.

Journal Article Reprod Sci · September 2021 Uterine fibroids feature excessive deposition of types I and III collagen. Previous ex vivo studies showed an FDA-approved collagenase (EN3835)-digested types I and III collagen fibers in fibroid tissues; however, collagenase had not been evaluated in vivo ... Full text Link to item Cite

Extracellular matrix and Hippo signaling as therapeutic targets of antifibrotic compounds for uterine fibroids.

Journal Article Clin Transl Med · July 2021 BACKGROUND: Uterine fibroids are highly prevalent, collagen-rich, mechanically stiff, fibrotic tumors for which new therapeutic options are needed. Increased extracellular matrix (ECM) stiffness activates mechanical signaling and Hippo/YAP promoting fibroi ... Full text Link to item Cite

Evaluation of Host Immune Cellular and Extracellular Matrix Responses to Prolapse Mesh With and Without Tension in a Rat Model.

Journal Article Female Pelvic Med Reconstr Surg · February 1, 2021 OBJECTIVES: We sought to determine whether vaginal host immune cellular and extracellular matrix responses are altered in a rat sacrocolpopexy model when lightweight polypropylene mesh is attached on tension versus without tension. METHODS: We performed hy ... Full text Link to item Cite

Polycarbonate Urethane Mesh: A New Material for Pelvic Reconstruction.

Journal Article Female Pelvic Med Reconstr Surg · February 1, 2021 OBJECTIVE: Polycarbonate urethane (PCU) is a new biomaterial, and its mechanical properties can be tailored to match that of vaginal tissue. We aimed to determine whether vaginal host immune and extracellular matrix responses differ after PCU versus lightw ... Full text Link to item Cite

Summary of the Proceedings of the Basic Science of Uterine Fibroids Meeting: New Developments February 28, 2020.

Journal Article F S Sci · February 2021 Scientists from multiple basic disciplines and an international group of physician-scientists from the field of obstetrics and gynecology presented recent studies and discussed new and evolving theories of uterine fibroid etiology, growth and development a ... Full text Link to item Cite

Evidence of biomechanical and collagen heterogeneity in uterine fibroids.

Journal Article PLoS One · 2019 OBJECTIVE: Uterine fibroids (leiomyomas) are common benign tumors of the myometrium but their molecular pathobiology remains elusive. These stiff and often large tumors contain abundant extracellular matrix (ECM), including large amounts of collagen, and c ... Full text Link to item Cite

Biochemical Pathways and Myometrial Cell Differentiation Leading to Nodule Formation Containing Collagen and Fibronectin.

Journal Article Curr Protein Pept Sci · 2017 Utilizing both primary myometrial cells and a myometrial cell line, we show here that myometrial cells undergo transition to a myofibroblast-like phenotype after a biological insult of 72 hours serum starvation and serum add-back (SB: 1% to 10% FBS). We al ... Full text Link to item Cite

Loss of stiffness in collagen-rich uterine fibroids after digestion with purified collagenase Clostridium histolyticum.

Journal Article Am J Obstet Gynecol · November 2016 BACKGROUND: Uterine fibroids are a significant health problem. These common benign tumors occur in 70-80% of women before age 50 years and often cause bleeding and pain and can interfere considerably with daily life. Current treatment options are limited. ... Full text Link to item Cite

Inflammatory Response of Human Gestational Membranes to Ureaplasma parvum Using a Novel Dual-Chamber Tissue Explant System.

Journal Article Biol Reprod · May 2016 Preterm premature rupture of membranes (PPROM) is often associated with intra-amniotic inflammation and infection. Current understanding of the pathogenesis of PPROM includes activation of pro-inflammatory cytokines and proteolytic enzymes leading to compr ... Full text Link to item Cite

341: Novel ex vivo system to evaluate inflammation of gestational membranes in response to U. parvum

Conference American Journal of Obstetrics and Gynecology · January 2016 Full text Cite

Injectable Clostridium histolyticum collagenase as a potential treatment for uterine fibroids.

Journal Article Reprod Sci · December 2014 Purified Clostridium histolyticum collagenase (CHC), an Food and Drug Administration-approved drug that does not affect nerves or blood vessels, was assessed as a potential treatment for fibroids in this proof-of-principle study. Fibroids (1-4 cm, capsules ... Full text Link to item Cite

The naturally occurring luteinizing hormone surge is diminished in mice lacking estrogen receptor Beta in the ovary.

Journal Article Biol Reprod · February 2014 Female ESR2-null mice (betaERKO) display defects in ovarian function and are subfertile. Follicular maturation is impaired and explains smaller litters, but betaERKO also produce fewer litters, which may be partially due to inadequate ovulatory signals. To ... Full text Link to item Cite

The extracellular matrix contributes to mechanotransduction in uterine fibroids.

Journal Article Obstet Gynecol Int · 2014 The role of the extracellular matrix (ECM) and mechanotransduction as an important signaling factor in the human uterus is just beginning to be appreciated. The ECM is not only the substance that surrounds cells, but ECM stiffness will either compress cell ... Full text Open Access Link to item Cite

Organotypic 3D-culture of uterine fibroid cells

Conference GLYCOBIOLOGY · November 1, 2012 Link to item Cite

Interdisciplinary research career development: building interdisciplinary research careers in women's health program best practices.

Journal Article J Womens Health (Larchmt) · November 2011 BACKGROUND: The Office of Research on Women's Health (ORWH) and the National Institutes of Health (NIH) Institutes and Centers and the Agency for Health Care Research and Quality (AHRQ) have sponsored an interdisciplinary research career development progra ... Full text Link to item Cite

CDB-4124 does not cause apoptosis in cultured fibroid cells.

Journal Article Reprod Sci · September 2011 OBJECTIVE: Selective progesterone receptor modulators (SPRMs), such as asoprisnil (J867) and ulipristal (CDB-2914), have been shown to reduce fibroid volume in vivo and to induce apoptosis in vitro. CDB-4124 (telapristone), a SPRM with different side group ... Full text Link to item Cite

Temperature-responsive biocompatible copolymers incorporating hyperbranched polyglycerols for adjustable functionality.

Journal Article Journal of functional biomaterials · August 2011 Temperature-triggered copolymers are proposed for a number of bio-applications but there is no ideal material platform, especially for injectable drug delivery. Options are needed for degradable biomaterials that not only respond to temperature but also ea ... Full text Cite

Biological and biochemical consequences of global deletion of exon 3 from the ER alpha gene.

Journal Article FASEB J · December 2010 To address issues resulting from α estrogen receptor-knockout (αERKO) residual N-terminal truncated estrogen receptor α, and to allow tissue-selective deletion of ERα, we generated loxP-flanked exon 3 mice. Initial characterization of global sox2 cre-deriv ... Full text Link to item Cite

Insufficient luteinizing hormone-induced intracellular signaling disrupts ovulation in preovulatory follicles lacking estrogen receptor-{beta}.

Journal Article Endocrinology · June 2010 Gonadotropin-stimulated estrogen receptor-beta (ERbeta)-null preovulatory follicles exhibit submaximal estradiol production, insufficient acquisition of LH receptor, and attenuated expression of essential ovulatory genes. These observations lead to low ovu ... Full text Link to item Cite

Proellex Does Not Cause Apoptosis in Cultured Fibroid Cells.

Conference REPRODUCTIVE SCIENCES · March 1, 2010 Link to item Cite

Opposing LSD1 complexes function in developmental gene activation and repression programmes.

Journal Article Nature · April 19, 2007 Precise control of transcriptional programmes underlying metazoan development is modulated by enzymatically active co-regulatory complexes, coupled with epigenetic strategies. One thing that remains unclear is how specific members of histone modification e ... Full text Link to item Cite

The bi-modal effects of estradiol on gonadotropin synthesis and secretion in female mice are dependent on estrogen receptor-alpha.

Journal Article J Endocrinol · October 2006 Depending on the estrous/menstrual cycle stage in females, ovarian-derived estradiol (E(2)) exerts either a negative or a positive effect on the hypothalamic-pituitary axis to regulate the synthesis and secretion of pituitary gonadotropins, LH, and FSH. To ... Full text Link to item Cite

Phthalate toxicity in the ovary

Chapter · 2004 The only known text that deals specifically with toxicity in the ovary, Ovarian Toxicology updates our current understanding of the effects of environmental chemicals on ovarian function. ... Cite

Equatorial segment protein defines a discrete acrosomal subcompartment persisting throughout acrosomal biogenesis.

Journal Article Biol Reprod · September 2003 The equatorial segment of the acrosome underlies the domain of the sperm that fuses with the egg membrane during fertilization. Equatorial segment protein (ESP), a novel 349-amino acid concanavalin-A-binding protein encoded by a two-exon gene (SP-ESP) loca ... Full text Link to item Cite

SAMP14, a novel, acrosomal membrane-associated, glycosylphosphatidylinositol-anchored member of the Ly-6/urokinase-type plasminogen activator receptor superfamily with a role in sperm-egg interaction.

Journal Article J Biol Chem · August 15, 2003 We report a new member of the Ly-6/urokinase-type plasminogen activator receptor (uPAR) superfamily of receptors, SAMP14, which is retained on the inner acrosomal membrane of the human spermatozoan following the acrosome reaction and may play a role in fer ... Full text Link to item Cite

SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa.

Journal Article Biol Reprod · May 2003 We report the presence of a unique, non-bacteriolytic, c (chicken or conventional type) lysozyme-like protein, SLLP1, in the acrosome of human sperm. C lysozymes are bacteriolytic and can also bind to N-acetylglucosamines linked by beta-1,4 glycosidic bond ... Full text Link to item Cite

ePAD, an oocyte and early embryo-abundant peptidylarginine deiminase-like protein that localizes to egg cytoplasmic sheets.

Journal Article Dev Biol · April 1, 2003 Selected for its high relative abundance, a protein spot of MW approximately 75 kDa, pI 5.5 was cored from a Coomassie-stained two-dimensional gel of proteins from 2850 zona-free metaphase II mouse eggs and analyzed by tandem mass spectrometry (TMS), and n ... Full text Link to item Cite

Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation.

Journal Article J Biol Chem · March 28, 2003 Before fertilization can occur, mammalian sperm must undergo capacitation, a process that requires a cyclic AMP-dependent increase in tyrosine phosphorylation. To identify proteins phosphorylated during capacitation, two-dimensional gel analysis coupled to ... Full text Link to item Cite

Differential extraction and enrichment of human sperm surface proteins in a proteome: identification of immunocontraceptive candidates.

Journal Article Electrophoresis · August 2001 The objective of this study was to discover previously unknown human sperm surface proteins that may be candidate contraceptive vaccinogens. To this end, methods of concentrating human sperm proteins for microsequencing by mass spectrometry were used, whic ... Full text Link to item Cite

Outer dense fiber proteins are dominant postobstruction autoantigens in adult Lewis rats.

Journal Article Biol Reprod · May 2001 Obstruction of the male reproductive tract commonly results in generation of antisperm autoantibodies. However, only a few of the sperm autoantigens recognized by these antibodies have been characterized. To identify postobstruction rat sperm autoantigens, ... Full text Link to item Cite

Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath.

Journal Article J Androl · 2001 Dysplasia of the fibrous sheath (DFS) is characterized by male infertility, asthenozoospermia, and morphologically abnormal flagella that possess a severely malformed fibrous sheath. In many cases, DFS is familial, suggesting a genetic component. Human AKA ... Link to item Cite

APL17 and APLX: Two novel human acrosomal pseudo-lysozymes

Conference MOLECULAR BIOLOGY OF THE CELL · December 1, 2000 Link to item Cite

Calcium ions positively modulate follicle-stimulating hormone- and exogenous cyclic 3',5'-adenosine monophosphate-driven transcription of the P450(scc) gene in porcine granulosa cells.

Journal Article Endocrinology · July 2000 Given the evident modulation of FSH-induced steroidogenesis by Ca2+ in granulosa cells, we here test the hypothesis that Ca2+ controls expression of the enzymatically rate-limiting cytochrome P450(scc) (CYP11A) gene. To test this postulate, we quantitated ... Full text Link to item Cite

Luteinizing hormone (LH) drives diverse intracellular calcium second messenger signals in isolated porcine ovarian thecal cells: preferential recruitment of intracellular Ca2+ oscillatory cells by higher concentrations of LH.

Journal Article Endocrinology · June 2000 The present study examines Ca2+ second messenger signaling driven by LH in isolated porcine thecal cells. To this end, we implemented semiquantitative fluorescent (fura-2) videomicroscopic imaging of single thecal cells in vitro. Stimulation of 388 cells w ... Full text Link to item Cite

Influence of vitamin A injection before mating on oocyte development, follicular hormones, and ovulation in gilts fed high-energy diets.

Journal Article J Anim Sci · June 2000 Previous research revealed that treatment with vitamin A approximately 5 d before ovulation may increase litter size in weaned sows and improve embryonal survival in gilts fed high-energy diets that reduced embryonal survival. For the current study, the hy ... Full text Link to item Cite

Expression of parathyroid hormone-related peptide (PTH-rp) and its receptorin the porcine ovary: regulation by transforming growth factor-beta and possible paracrine effects of granulosa cell PTH-rp secretion on theca cells.

Journal Article Biol Reprod · February 2000 Parathyroid hormone-related peptide (PTH-rp) and the PTH-rp receptor are expressed in certain cancers as well as in many normal tissues. To evaluate the expression of this Ca(2+)-regulating hormone and its receptor in porcine ovary, we isolated partial cDN ... Full text Link to item Cite

Role of gonadotropin-releasing hormone pulse frequency in differential regulation of gonadotropins in the gilt.

Journal Article Biol Reprod · April 1997 We tested the hypothesis that different GnRH pulse frequencies will affect serum LH and FSH differently. Ovariectomized gilts (n = 6), immunized against GnRH, were given 200-ng pulses of GnRH agonist (GnRH-A) every 180 min for 3 days (pretreatment), follow ... Full text Link to item Cite

Transdermal iontophoretic delivery of luteinizing hormone releasing hormone (LHRH): effect of repeated administration.

Journal Article Pharm Res · July 1994 The transdermal iontophoretic delivery of the reproductive peptide hormone, luteinizing hormone releasing hormone (LHRH) is investigated in the isolated perfused porcine skin flap model (IPPSF). LHRH is delivered twice in a single flap experiment in effort ... Full text Link to item Cite

Transdermal iontophoretic peptide delivery: in vitro and in vivo studies with luteinizing hormone releasing hormone.

Journal Article J Pharm Sci · May 1993 Protein and peptide drugs are not orally active. Their large molecular size and charged character make them poor candidates for passive transdermal delivery. With an applied electromotive force, these drugs can be forced through the skin to be absorbed by ... Full text Link to item Cite

Transdermal lontophoretic peptide delivery: In vitro and in vivo studies with luteinizing hormone releasing hormone

Journal Article Journal of Pharmaceutical Sciences · January 1, 1993 Protein and peptide drugs are not orally active. Their large molecular size and charged character make them poor candidates for passive transdermal delivery. With an applied electromotive force, these drugs can be forced through the skin to be absorbed by ... Full text Cite