Scholars@Duke
Mario Gonzalez-Gronow

Mario Gonzalez-Gronow

Associate Professor Emeritus of Pathology
Pathology
gonza002@mc.duke.edu
Duke Box 3712, Durham, NC 27710
304 Davison Bldg, Durham, NC 27710

Selected Publications

Physiological Roles of the Autoantibodies to the 78-Kilodalton Glucose-Regulated Protein (GRP78) in Cancer and Autoimmune Diseases.

Journal Article Biomedicines · May 24, 2022 The 78 kDa glucose-regulated protein (GRP78), a member of the 70 kDa heat-shock family of molecular chaperones (HSP70), is essential for the regulation of the unfolded protein response (UPR) resulting from cellular endoplasmic reticulum (ER) stress. During ... Full text Link to item Cite

Glucose-regulated protein (GRP78) is an important cell surface receptor for viral invasion, cancers, and neurological disorders.

Journal Article IUBMB life · June 2021 The 78 kDa glucose-regulated protein (GRP78) is an endoplasmic reticulum (ER)-resident molecular chaperone. GRP78 is a member of the 70 kDa heat shock family of proteins involved in correcting and clearing misfolded proteins in the ER. In response to cellu ... Full text Open Access Cite

Novel Cell Surface Targets for the Plasminogen Activating System in the Brain: Implications for Human Disease

Chapter · January 1, 2018 The plasminogen (Pg) activator system plays an important role in the central nervous system (CNS), including processes of neuronal migration, neurite outgrowth, and neuronal plasticity. Pg and tissue-type plasminogen activator (t-PA) are primarily involved ... Full text Cite

Autoantibodies against the cell surface-associated chaperone GRP78 stimulate tumor growth via tissue factor.

Journal Article J Biol Chem · December 22, 2017 Tumor cells display on their surface several molecular chaperones that normally reside in the endoplasmic reticulum. Because this display is unique to cancer cells, these chaperones are attractive targets for drug development. Previous epitope-mapping of a ... Full text Link to item Cite

Myelin basic protein stimulates plasminogen activation via tissue plasminogen activator following binding to independent l-lysine-containing domains.

Journal Article Biochem Biophys Res Commun · August 26, 2017 Myelin basic protein (MBP) is a key component of myelin, the specialized lipid membrane that encases the axons of all neurons. Both plasminogen (Pg) and tissue-type plasminogen activator (t-PA) bind to MBP with high affinity. We investigated the kinetics a ... Full text Link to item Cite

Activated α2-Macroglobulin Regulates Transcriptional Activation of c-MYC Target Genes through Cell Surface GRP78 Protein.

Journal Article J Biol Chem · May 13, 2016 Activated α2-macroglobulin (α2M*) signals predominantly through cell surface GRP78 (CS-GRP78) to promote proliferation and survival of cancer cells; however, the molecular mechanism remains obscure. c-MYC is an essential transcriptional regulator that cont ... Full text Link to item Cite

Adult-Onset Ligneous Conjunctivitis with Detection of a Novel Plasminogen Gene Mutation and Anti-Plasminogen IgA Antibody: A Clinicopathologic Study and Review of Literature.

Journal Article Semin Ophthalmol · 2016 PURPOSE: To report a novel plasminogen gene mutation and detection of anti-plasminogen antibodies in a patient with ligneous conjunctivitis successfully treated with 60% fresh frozen plasma (FFP). METHODS: Retrospective data collected on a 45-year-old Cauc ... Full text Link to item Cite

Catalytic autoantibodies against myelin basic protein (MBP) isolated from serum of autistic children impair in vitro models of synaptic plasticity in rat hippocampus.

Journal Article J Neuroimmunol · October 15, 2015 Autoantibodies from autistic spectrum disorder (ASD) patients react with multiple proteins expressed in the brain. One such autoantibody targets myelin basic protein (MBP). ASD patients have autoantibodies to MBP of both the IgG and IgA classes in high tit ... Full text Link to item Cite

Binding of tissue-type plasminogen activator to the glucose-regulated protein 78 (GRP78) modulates plasminogen activation and promotes human neuroblastoma cell proliferation in vitro.

Journal Article J Biol Chem · September 5, 2014 The glucose-regulated protein 78 (GRP78) is a plasminogen (Pg) receptor on the cell surface. In this study, we demonstrate that GRP78 also binds the tissue-type plasminogen activator (t-PA), which results in a decrease in K(m) and an increase in the V(max) ... Full text Link to item Cite

The voltage-dependent anion channel (VDAC) binds tissue-type plasminogen activator and promotes activation of plasminogen on the cell surface.

Journal Article J Biol Chem · January 4, 2013 The voltage-dependent anion channel (VDAC), a major pore-forming protein in the outer membrane of mitochondria, is also found in the plasma membrane of a large number of cells where in addition to its role in regulating cellular ATP release and volume cont ... Full text Link to item Cite

Changes in oligosaccharide chains of autoantibodies to GRP78 expressed during progression of malignant melanoma stimulate melanoma cell growth and survival.

Journal Article Melanoma Res · August 2011 A correlation between expression of the glucose-regulated protein of 78 kDa (GRP78) in malignant melanoma tumors and poor patient survival is well established. In this study, in addition to demonstrating the expression of GRP78 in tumor tissue, we investig ... Full text Link to item Cite

Autoantibodies against cell surface GRP78 promote tumor growth in a murine model of melanoma.

Journal Article Melanoma Res · February 2011 Autoantibodies that react with GRP78 expressed on the cell-surface of many tumor cell lines occur in the sera of patients with prostate cancer, melanoma, and ovarian cancer. These autoantibodies are a negative prognostic factor in prostate cancer and, when ... Full text Link to item Cite

Antibodies against the voltage-dependent anion channel (VDAC) and its protective ligand hexokinase-I in children with autism.

Journal Article J Neuroimmunol · October 8, 2010 Featured Publication Autistic children show elevated serum levels of autoantibodies to several proteins essential for the function of normal brains. The voltage-dependent anion channel (VDAC) and hexokinase-I, a VDAC protective ligand, were identified as targets of this autoim ... Full text Link to item Cite

Binding of anti-GRP78 autoantibodies to cell surface GRP78 increases tissue factor procoagulant activity via the release of calcium from endoplasmic reticulum stores.

Journal Article J Biol Chem · September 10, 2010 Featured Publication The increased risk of venous thromboembolism in cancer patients has been attributed to enhanced tissue factor (TF) procoagulant activity (PCA) on the surface of cancer cells. Recent studies have shown that TF PCA can be modulated by GRP78, an endoplasmic r ... Full text Link to item Cite

Patterns of GRP78 and MTJ1 expression in primary cutaneous malignant melanoma.

Journal Article Mod Pathol · January 2010 Featured Publication Cell surface expression of glucose-regulated protein 78 (GRP78) occurs in several types of cancer; however, its role in the behavior of primary cutaneous melanoma is not well studied. The association of cell surface GRP78 with other proteins such as MTJ1 s ... Full text Link to item Cite

GRP78: a multifunctional receptor on the cell surface.

Journal Article Antioxid Redox Signal · September 2009 Featured Publication The 78 kDa glucose-regulated protein (GRP78) is an endoplasmic reticulum chaperone, whose function is generally thought to be restricted to controlling the structural maturation of nascent glycoproteins. However, GRP78 also is expressed on the cell surface ... Full text Link to item Cite

Dipeptidyl peptidase IV (DPP IV/CD26) is a cell-surface plasminogen receptor.

Journal Article Front Biosci · January 1, 2008 Featured Publication Binding of plasminogen (Pg) to cell-surface receptors colocalized with plasminogen activators promotes Pg activation and enables cells to utilize the proteolytic activity of plasmin (Pm). Proteolysis by Pm is necessary in several physiological and patholog ... Full text Link to item Cite

GRP 78 protein expression in malignant melanoma

Journal Article JOURNAL OF CUTANEOUS PATHOLOGY · January 1, 2008 Link to item Cite

Plasminogen structural domains exhibit different functions when associated with cell surface GRP78 or the voltage-dependent anion channel.

Journal Article J Biol Chem · November 9, 2007 Featured Publication Both the voltage-dependent anion channel and the glucose-regulated protein 78 have been identified as plasminogen kringle 5 receptors on endothelial cells. In this study, we demonstrate that kringle 5 binds to a region localized in the N-terminal domain of ... Full text Link to item Cite

Prostate cancer cell proliferation in vitro is modulated by antibodies against glucose-regulated protein 78 isolated from patient serum.

Journal Article Cancer Res · December 1, 2006 Featured Publication Circulating autoantibodies against the glucose-regulated protein of 78 kDa (GRP78) are present at high levels in prostate cancer patients and are a biomarker of aggressive tumor behavior. We purified the anti-GRP78 IgGs and examined their effect on 1-LN, P ... Full text Link to item Cite

Anti-tumor necrosis factor-alpha therapy augments dipeptidyl peptidase IV activity and decreases autoantibodies to GRP78/BIP and phosphoglucose isomerase in patients with rheumatoid arthritis.

Journal Article J Rheumatol · November 2005 Featured Publication OBJECTIVE: To assess the enzymatic activity and biochemical status of dipeptidyl peptidase IV (DPP IV), an enzyme that participates in the degradation of proinflammatory molecules, in sera from a group of patients with rheumatoid arthritis (RA; n = 15) tre ... Link to item Cite

Angiostatin's molecular mechanism: aspects of specificity and regulation elucidated.

Journal Article J Cell Biochem · October 1, 2005 Featured Publication Tumor growth requires the development of new vessels that sprout from pre-existing normal vessels in a process known as "angiogenesis" [Folkman (1971) N Engl J Med 285:1182-1186]. These new vessels arise from local capillaries, arteries, and veins in respo ... Full text Link to item Cite

Association of plasminogen with dipeptidyl peptidase IV and Na+/H+ exchanger isoform NHE3 regulates invasion of human 1-LN prostate tumor cells.

Journal Article J Biol Chem · July 22, 2005 Featured Publication Binding of plasminogen type II (Pg 2) to dipeptidyl peptidase IV (DPP IV) on the surface of the highly invasive 1-LN human prostate tumor cell line induces an intracellular Ca2+ ([Ca2+]i) signaling cascade accompanied by a rise in intracellular pH (pHi). I ... Full text Link to item Cite

The role of MTJ-1 in cell surface translocation of GRP78, a receptor for alpha 2-macroglobulin-dependent signaling.

Journal Article J Immunol · February 15, 2005 Featured Publication MTJ-1 associates with a glucose-regulated protein of Mr approximately 78,000(GRP78) in the endoplasmic reticulum and modulates GRP78 activity as a chaperone. GRP78 also exists on the cell surface membrane, where it is associated with a number of functions. ... Full text Link to item Cite

Angiostatin directly inhibits human prostate tumor cell invasion by blocking plasminogen binding to its cellular receptor, CD26.

Journal Article Exp Cell Res · February 1, 2005 Featured Publication Previous studies demonstrate that one of the six plasminogen type 2 glycoforms, plasminogen 2epsilon, enhances invasiveness of the 1-LN human prostate tumor cell line in an in vitro model. Binding of plasminogen 2epsilon to CD26 on the cell surface induces ... Full text Link to item Cite

Behçet's disease patients present high levels of deglycosylated anti-lipoteichoic acid IgG and high IL-8 production after lipoteichoic acid stimulation.

Journal Article Clin Exp Rheumatol · 2005 Featured Publication OBJECTIVES: Lipoteichoic acid (LTA), induces some of the clinical symptoms of Behçet's disease (BD) in a rat animal model. These results led to the hypothesis that LTA may also trigger BD in humans. We investigated the humoral and cellular immune response ... Link to item Cite

A novel receptor function for the heat shock protein Grp78: silencing of Grp78 gene expression attenuates alpha2M*-induced signalling.

Journal Article Cell Signal · August 2004 Featured Publication The activated proteinase inhibitor alpha2-macroglobulin (alpha2M*) binds to two receptors, the low density lipoprotein receptor-related protein (LRP-1) and the alpha2M* signalling receptor (alpha2MSR). Silencing LRP-1 gene expression in macrophages by RNA ... Full text Link to item Cite

Cell surface adenosine deaminase binds and stimulates plasminogen activation on 1-LN human prostate cancer cells.

Journal Article J Biol Chem · May 14, 2004 Featured Publication Adenosine deaminase (ADA) is expressed intracellularly by all cells, but in some tissues, it is also associated with the cell surface multifunctional glycoprotein CD26/dipeptidyl peptidase IV. By modulating extracellular adenosine, this "ecto-ADA" may regu ... Full text Link to item Cite

The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cells.

Journal Article J Biol Chem · July 18, 2003 Human plasminogen contains structural domains that are termed kringles. Proteolytic cleavage of plasminogen yields kringles 1-3 or 4 and kringle 5 (K5), which regulate endothelial cell proliferation. The receptor for kringles 1-3 or 4 has been identified a ... Full text Link to item Cite

Streptokinase promotes development of dipeptidyl peptidase IV (CD26) autoantibodies after fibrinolytic therapy in myocardial infarction patients.

Journal Article Clin Diagn Lab Immunol · November 2002 Dipeptidyl peptidase IV (DPP IV) (CD26) plays a critical role in the modulation and expression of autoimmune and inflammatory diseases. We recently reported that sera from patients with rheumatoid arthritis and systemic lupus erythematosus contained low le ... Full text Link to item Cite

The role of Grp 78 in alpha 2-macroglobulin-induced signal transduction. Evidence from RNA interference that the low density lipoprotein receptor-related protein is associated with, but not necessary for, GRP 78-mediated signal transduction.

Journal Article J Biol Chem · November 1, 2002 The low density lipoprotein receptor-related protein (LRP) is a scavenger receptor that binds to many proteins, some of which trigger signal transduction. Receptor-recognized forms of alpha(2)-Macroglobulin (alpha(2)M*) bind to LRP, but the pattern of sign ... Full text Link to item Cite

Tissue factor is the receptor for plasminogen type 1 on 1-LN human prostate cancer cells.

Journal Article Blood · June 15, 2002 Tissue factor (TF), the initiator of the extrinsic pathway of coagulation, binds plasminogen (Pg) with high affinity through an interaction between kringles 1-3 of Pg and the extracellular domain of TF. We investigated the binding of Pg type 1 (Pg 1) and P ... Full text Link to item Cite

Interaction of plasminogen with dipeptidyl peptidase IV initiates a signal transduction mechanism which regulates expression of matrix metalloproteinase-9 by prostate cancer cells.

Journal Article Biochem J · April 15, 2001 Both plasminogen (Pg) activation and matrix metalloproteinases (MMPs) are involved in the proteolytic degradation of extracellular matrix components, a requisite event for malignant cell metastasis. The highly invasive 1-LN human prostate tumour cell line ... Full text Link to item Cite

Characterization of human serum dipeptidyl peptidase IV (CD26) and analysis of its autoantibodies in patients with rheumatoid arthritis and other autoimmune diseases.

Journal Article Clin Exp Rheumatol · 2001 OBJECTIVES: To assess the serum levels, specific activity and other characteristics of dipeptidylpeptidase IV (DPP IV/CD26), an ectoenzyme that plays a critical role in the modulation and expression of autoimmune and inflammatory diseases, from patients wi ... Link to item Cite

Selective upregulated expression of the alpha2-macroglobulin signaling receptor in highly metastatic 1-LN prostate carcinoma cells.

Journal Article Arch Biochem Biophys · November 1, 2000 Cellular binding of receptor-recognized forms of alpha2-macroglobulin (alpha2M*) is mediated by the low-density lipoprotein receptor related protein (LRP) and the alpha2M signaling receptor (alpha2MSR). In nonmalignant cells, ligation of alpha2MSR promotes ... Full text Link to item Cite

Coordinate regulation of the alpha(2)-macroglobulin signaling receptor and the low density lipoprotein receptor-related protein/alpha(2)-macroglobulin receptor by insulin.

Journal Article J Biol Chem · September 3, 1999 We have studied insulin-dependent regulation of macrophage alpha(2)-macroglobulin signaling receptors (alpha(2)MSR) and low density lipoprotein receptor-related protein/alpha(2)M receptors (LRP/alpha(2)MR) employing cell binding of (125)I-alpha(2)M*, inhib ... Full text Link to item Cite

Upregulation of a2m signalling receptor by insulin in macrophages

Journal Article FASEB Journal · December 1, 1998 In 1984 this laboratory showed that the treatment of adipocytes and fibrob lasts with insulin enhanced the binding of 125I-a2M-methylamine by about 2-3 fold and its slowed receptor-mediated degradation. We have recently reported the presence of a novel ojM ... Cite

Secretion of MMP-9 by 1-LN human prostate tumor cells is modulated via a signaling mechanism initiated by interaction between plasminogen carbohydrate chains and DPP IV (CD26) on the cell surface

Journal Article FASEB Journal · December 1, 1998 Remodeling of the extracellular matrix (ECM), which occurs during metastatic processes, is one of the requisite events for cellular invasion. Both the plasminogen (Pg) activation system and matrix metalloproteinases (MMPs) are involved in proteolytic degra ... Cite

Dipeptidyl peptidase IV (CD26) is a receptor for streptokinase on rheumatoid synovial fibroblasts

Journal Article Fibrinolysis and Proteolysis · January 1, 1998 Plasminogen (Pg) binds to synovial fibroblasts from patients with rheumatoid arthritis with a very high affinity and in a dose-dependent manner. The Pg receptor in these cells is composed of a glycoprotein IIb/IIIa related protein (α(IIIb)β3) in associatio ... Full text Cite

Plasmin(ogen) carbohydrate chains mediate binding to dipeptidyl peptidase IV (CD 26) in rheumatoid arthritis human synovial fibroblasts

Journal Article Fibrinolysis and Proteolysis · January 1, 1998 Objective: To assess the reactivity between dipeptidyl peptidase IV (DPP IV) and the α2,3-linked sialic acid of the plasminogen (Pg)Thr345 O- linked carbohydrate chain as the mechanism enabling plasmin (Pm) to induce intracellular Ca2+ via DPP IV on rheuma ... Full text Cite

Molecular abnormalities of human plasminogen isolated from synovial fluid of rheumatoid arthritis patients.

Journal Article J Mol Med (Berl) · May 1997 Plasminogen (Pg) in the synovial fluid of patients with acute inflammatory disease, including rheumatoid arthritis, osteoarthritis, and gout, was purified by affinity chromatography techniques. The Pg isolated from patients with osteoarthritis and gout has ... Link to item Cite

Up-regulation of the alpha2-macroglobulin signaling receptor on rheumatoid synovial fibroblasts.

Journal Article J Biol Chem · January 3, 1997 In the present study, we demonstrate that the alpha2-macroglobulin (alpha2M) signaling receptor is up-regulated on rheumatoid synovial fibroblasts. In rheumatoid cells, 125I-alpha2M-methylamine bound to two sites; namely, one of high affinity (Kd approxima ... Full text Link to item Cite

Analysis of autoantibodies to plasminogen in the serum of patients with rheumatoid arthritis.

Journal Article J Mol Med (Berl) · August 1996 Sera from patients with rheumatoid arthritis containing high titers of anti-streptokinase antibodies were found to contain anti-plasminogen antibodies of the IgG and IgA classes. High titers of anti-plasminogen autoantibodies of the IgA class were also fou ... Full text Link to item Cite

ATP-regulated activity of the plasmin-streptokinase complex: a novel mechanism involving phosphorylation of streptokinase.

Journal Article Biochem J · January 1, 1996 Streptokinase, an extracellular protein produced by Streptococci, is capable of activating the human fibrinolytic zymogen plasminogen. The rate of amidolytic activity of the plasminogen-streptokinase complex is greatly diminished by micromolar concentratio ... Full text Link to item Cite

Potential pathogenicity of deglycosylated IgG cross reactive with streptokinase and fibronectin in the serum of patients with rheumatoid arthritis.

Journal Article J Rheumatol · January 1996 OBJECTIVE: Fibronectin (FN) and the streptococcal plasminogen activator streptokinase (SK) share the epitope LTSRPA. This epitope is not reactive in native FN and it reacts with anti-SK antibodies only after plasmin digestion of the protein. To investigate ... Link to item Cite

Plasmin(ogen) carbohydrate chains mediate binding to dipeptidyl peptidase iv (cd 26) on rheumatoid artrhitis human synovial fibroblasts

Journal Article Fibrinolysis · January 1, 1996 Both plasminogen (Pg) and the streptococcal plasminogen activator streptokinase (SK) bind to synovial fibroblasts from patients with rheumatoid arthritis (RA) with a very high affinity and in a dose-dependent manner. The receptor for both proteins has been ... Full text Cite

Interaction of human plasminogen kringles 1-3 (angiostatin) with human umbilical vein endothelial cells (huvec): binding properties and effects on cell proliferation

Journal Article Fibrinolysis · January 1, 1996 Receptors which bind plasminogen (Pg) have previously been demonstrated in HUVEC. To determine whether the mechanism by which the Pg fragment, angiostatin, inhibits endothelial cell migralion and/or proliferation involves interaction with the Pg receptor, ... Full text Cite

The role of carbohydrate in the activation of plasminogen 2 glycoforms by streptokinase

Journal Article Fibrinolysis · January 1, 1996 Plasminogen 2 has six glycoforms that differ in their sialic acid content. In this study we have examined the effect that this differential sialylation has on activation of these glycoforms by streptokinase (SK). We find that increases in sialic acid conte ... Full text Cite

Dipeptidyl Peptidase IV(CD 26) is a Receptor for Streptokinase and Fibronectin on Rheumatoid Arthritis Human Synovial Fibroblasts

Journal Article Fibrinolysis · January 1, 1996 Streptokinase (SK), a potent non-proteolytic plasminogen (Pg) activator, binds to synovial fibroblasts derived from patients with rheumatoid arthritis(RA) at very high affinity (Kd= 77 nM) either directly or via a complex with Pg. By contrast, SK does not ... Full text Cite

Characterization of the plasminogen receptors of normal and rheumatoid arthritis human synovial fibroblasts.

Journal Article J Biol Chem · February 11, 1994 Plasminogen (Pg) activation on the surface of rheumatoid arthritis (RA) synovial fibroblasts by the urinary-type Pg activator induced a significant increase in cytosolic free Ca2+ concentration. This response was not observed in normal synovial fibroblasts ... Link to item Cite

Plasminogen activation stimulates an increase in intracellular calcium in human synovial fibroblasts.

Journal Article J Biol Chem · October 5, 1993 Both plasminogen (Pg) and urinary-type Pg activator (u-PA), but not tissue-type Pg activator (t-PA), bind to normal and rheumatoid arthritis (RA) human synovial fibroblasts in culture with high affinity and in a dose-dependent manner. Single cell intracell ... Link to item Cite

Streptokinase and human fibronectin share a common epitope: implications for regulation of fibrinolysis and rheumatoid arthritis.

Journal Article Biochim Biophys Acta · January 22, 1993 Rheumatoid arthritis is a disease characterized by a destructive inflammatory process in joints. Fibronectin (FN) is present at a high concentration in rheumatoid synovial tissue and it is a chemoattractant for inflammatory cells. FN fragments also play si ... Full text Link to item Cite

Effect of desialylation on the biological properties of human plasminogen.

Journal Article Biochem J · May 15, 1992 There are two major isoenzymes of plasminogen (Pg) in human plasma, designated Pg1 and Pg2. Both Pg forms have an identical primary structure, but differ in their extent of glycosylation. Removal of the oligosaccharide chains alters the normal physiologica ... Full text Link to item Cite

Plasmin binding to the plasminogen receptor enhances catalytic efficiency and activates the receptor for subsequent ligand binding.

Journal Article Arch Biochem Biophys · May 1, 1991 Specific cell surface receptors for plasminogen (Pg) are expressed by a wide variety of cell types. The colocalization of receptors for Pg and its activators restricts plasmin (Pm) activity to specific sites and serves to promote fibrinolysis and local Pg ... Full text Link to item Cite

The effect of divalent cations on the conformation and function of human plasminogen.

Journal Article Arch Biochem Biophys · January 1991 The activation of native human plasminogen (Glu1-Pg) by tissue plasminogen activator, urinary plasminogen activator (u-PA), and streptokinase is inhibited by the divalent cations Ca2+, Mg2+, and Mn2+. This inhibition is accompanied by a conformational chan ... Full text Link to item Cite

The role of carbohydrate in the function of human plasminogen: comparison of the protein obtained from molecular cloning and expression in Escherichia coli and COS cells.

Journal Article Biochim Biophys Acta · July 6, 1990 A cDNA library was constructed in the phage lambda gt11 from human liver mRNA enriched for plasminogen mRNA by chromatography on Sepharose 4B. A full-length cDNA clone of human plasminogen was isolated. The 2.7 kb cDNA encoded the entire plasminogen molecu ... Full text Link to item Cite

Neonatal plasminogen displays altered cell surface binding and activation kinetics. Correlation with increased glycosylation of the protein.

Journal Article J Clin Invest · July 1990 Plasminogen isolated from 60 full-term newborns differs from adult plasminogen in carbohydrate composition, kinetic activation constants, and cell binding. Amino acid composition and amino-terminal sequence analysis data indicate that the plasminogens of n ... Full text Link to item Cite

Regulation of plasminogen activation by components of the extracellular matrix.

Journal Article Biochemistry · May 22, 1990 The kinetics of activation of Glu-plasminogen (Glu-Pg) and Lys77-Pg by two-chain recombinant tissue plasminogen activator (t-PA) were determined in the presence of isolated protein components of the extracellular matrix (ECM) and compared to activation in ... Full text Link to item Cite

Lipoprotein a inhibits streptokinase-mediated activation of human plasminogen.

Journal Article Biochemistry · March 21, 1989 Lipoprotein a [Lp(a)] inhibits human plasminogen (Pg) conversion to plasmin (Pm) by streptokinase- (SK-) mediated activation. Kinetic and binding studies indicate that Lp(a) inhibits Pg activation by competitive and uncompetitive inhibition. Lp(a) competes ... Full text Link to item Cite

Further characterization of the cellular plasminogen binding site: evidence that plasminogen 2 and lipoprotein a compete for the same site.

Journal Article Biochemistry · March 21, 1989 Specific cell surface receptors for plasminogen (Pg) are expressed by a wide variety of cell types and serve to promote fibrinolysis and local Pg proteolysis. Pg types 1 and 2, separated by chromatography on concanavalin A-Sepharose, were utilized to deter ... Full text Link to item Cite

In vitro biosynthesis of plasminogen in a cell-free system directed by mRNA fractions isolated from monkey liver.

Journal Article Biochemistry · January 17, 1984 mRNA was isolated from total RNA of monkey liver by oligo(dT)-cellulose chromatography and was translated in a rabbit reticulocyte cell-free system. Analysis of the translation products immunoprecipitated with specific antibodies to monkey plasma plasminog ... Full text Link to item Cite

Mechanism of activation of human plasminogen by the activator complex, streptokinase-plasmin.

Journal Article J Biol Chem · February 25, 1978 The object of this investigation was to distinguish between two potential mechanisms of activation of human plasminogen (HPg) to plasmin (HPm) by catalytic levels of the activator complex, streptokinase.plasmin (SK.HPm). One mechanism, which is widely supp ... Link to item Cite

Purification and some properties of the Glu- and Lys-human plasmin heavy chains.

Journal Article J Biol Chem · April 10, 1977 The heavy polypeptide chains of human Glu-plasmin and human Lys-plasmin have been isolated in native solvents, after partial reduction and carboxymethylation of the corresponding plasmins. Two major forms of each heavy chain can be eluted, after adsorption ... Link to item Cite