The effect of divalent cations on the conformation and function of human plasminogen.
Publication
, Journal Article
Stack, S; Gonzalez-Gronow, M; Pizzo, SV
Published in: Arch Biochem Biophys
January 1991
The activation of native human plasminogen (Glu1-Pg) by tissue plasminogen activator, urinary plasminogen activator (u-PA), and streptokinase is inhibited by the divalent cations Ca2+, Mg2+, and Mn2+. This inhibition is accompanied by a conformational change in the molecule as evidenced by a decrease in Stokes' radius and intrinsic fluorescence. Kinetic analysis indicates that Mn2+ acts as an uncompetitive inhibitor of u-PA-catalyzed Glu1-Pg activation. In contrast to the inhibitory effects of divalent cations on Glu1-Pg, Ca2+ and Mg2+ stimulate the activation of proteolytically modified Lys77-Pg. These observations provide further evidence that Glu1-Pg and Lys77-Pg exhibit differential responses to ligands in the microenvironment.
Duke Scholars
Published In
Arch Biochem Biophys
DOI
ISSN
0003-9861
Publication Date
January 1991
Volume
284
Issue
1
Start / End Page
58 / 62
Location
United States
Related Subject Headings
- Urokinase-Type Plasminogen Activator
- Spectrometry, Fluorescence
- Protein Conformation
- Plasminogen
- Lysine
- Kinetics
- In Vitro Techniques
- Humans
- Glutamates
- Enzyme Activation
Citation
APA
Chicago
ICMJE
MLA
NLM
Stack, S., Gonzalez-Gronow, M., & Pizzo, S. V. (1991). The effect of divalent cations on the conformation and function of human plasminogen. Arch Biochem Biophys, 284(1), 58–62. https://doi.org/10.1016/0003-9861(91)90263-i
Stack, S., M. Gonzalez-Gronow, and S. V. Pizzo. “The effect of divalent cations on the conformation and function of human plasminogen.” Arch Biochem Biophys 284, no. 1 (January 1991): 58–62. https://doi.org/10.1016/0003-9861(91)90263-i.
Stack S, Gonzalez-Gronow M, Pizzo SV. The effect of divalent cations on the conformation and function of human plasminogen. Arch Biochem Biophys. 1991 Jan;284(1):58–62.
Stack, S., et al. “The effect of divalent cations on the conformation and function of human plasminogen.” Arch Biochem Biophys, vol. 284, no. 1, Jan. 1991, pp. 58–62. Pubmed, doi:10.1016/0003-9861(91)90263-i.
Stack S, Gonzalez-Gronow M, Pizzo SV. The effect of divalent cations on the conformation and function of human plasminogen. Arch Biochem Biophys. 1991 Jan;284(1):58–62.
Published In
Arch Biochem Biophys
DOI
ISSN
0003-9861
Publication Date
January 1991
Volume
284
Issue
1
Start / End Page
58 / 62
Location
United States
Related Subject Headings
- Urokinase-Type Plasminogen Activator
- Spectrometry, Fluorescence
- Protein Conformation
- Plasminogen
- Lysine
- Kinetics
- In Vitro Techniques
- Humans
- Glutamates
- Enzyme Activation