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Selected Publications


Multimodal segmentation of dynamic subcellular features using quantitative phase imaging and FRET-based sensors [Invited]

Journal Article Journal of the Optical Society of America A · November 1, 2024 Understanding cellular responses to mechanical environmental stimuli is important for cellular mechanotransduction studies. While fluorescence microscopy has been used for aiding mechanotransduction research due to its molecular sensitivity, the ab ... Full text Cite

Multiscale optical phase fluctuations link disorder strength and fractal dimension of cell structure.

Journal Article Biophysical journal · April 2023 Optical methods for examining cellular structure based on endogenous contrast rely on analysis of refractive index changes to discriminate cell phenotype. These changes can be visualized using techniques such as phase contrast microscopy, detected by light ... Full text Cite

Holography Cytometry: Imaging of Cells in Flow

Chapter · February 1, 2023 Holographic cytometry (HC) has been developed as an ultra-high throughput implementation of quantitative phase microscopy (QPM). While QPM has been well developed for studying cells based on endogenous contrast, few implementations have imaged cell ... Full text Cite

Multiscale Quantitative Phase Imaging Yields Fractal Dimension of Cellular Structures

Conference Novel Techniques in Microscopy, NTM 2023 · January 1, 2023 We demonstrate a novel technique for calculating the fractal dimension of adherent cells using multiscale Quantitative Phase Imaging information. We examine the relationship between this measurement and disorder strength across different cell lines. ... Full text Cite

Combined Quantitative Phase Microscopy and Förster Resonance Energy Transfer Imaging for Analyzing Endothelial Cell Shear Stress Response

Conference Optics InfoBase Conference Papers · January 1, 2022 Demonstration of quantitative phase microscopy and Förster resonance energy transfer for analyzing molecular and structural information simultaneously. We focus on signaling pathways and sub-cellular structure for endothelial cells exposed to various flows ... Cite

Deep imaging with 1.3 µm dual-axis optical coherence tomography and an enhanced depth of focus.

Journal Article Biomedical optics express · December 2021 For many clinical applications, such as dermatology, optical coherence tomography (OCT) suffers from limited penetration depth due primarily to the highly scattering nature of biological tissues. Here, we present a novel implementation of dual-axis optical ... Full text Cite

Automated Classification of Breast Cancer Cells Using High-Throughput Holographic Cytometry

Journal Article Frontiers in Physics · November 30, 2021 Holographic cytometry is an ultra-high throughput quantitative phase imaging modality that is capable of extracting subcellular information from millions of cells flowing through parallel microfluidic channels. In this study, we present our findings on the ... Full text Cite

Single Cell Analysis of Stored Red Blood Cells Using Ultra-High Throughput Holographic Cytometry.

Journal Article Cells · September 17, 2021 Holographic cytometry is introduced as an ultra-high throughput implementation of quantitative phase imaging of single cells flowing through parallel microfluidic channels. Here, the approach was applied for characterizing the morphology of individual red ... Full text Link to item Cite

Sensing Inhalation Injury-Associated Changes in Airway Wall Compliance by Anatomic Optical Coherence Elastography.

Journal Article IEEE transactions on bio-medical engineering · August 2021 Quantitative methods for assessing the severity of inhalation (burn) injury are needed to aid in treatment decisions. We hypothesize that it is possible to assess the severity of injuries on the basis of differences in the compliance of the airway wall. He ... Full text Cite

Combined quantitative phase microscopy and förster resonance energy transfer for analyzing cell ion dynamics

Conference Optics InfoBase Conference Papers · January 1, 2021 We demonstrate the use of quantitative phase microscopy and Förster resonance energy transfer for analyzing molecular and structural information simultaneously. Specifically, we focus on effects of ion channel modulation on signaling pathways and sub-cellu ... Cite

Localized compliance measurement of the airway wall using anatomic optical coherence elastography.

Journal Article Optics express · June 2019 We describe an elastographic method to circumferentially-resolve airway wall compliance using endoscopic, anatomic optical coherence tomography (aOCT) combined with an intraluminal pressure catheter. The method was first demonstrated on notched silicone ph ... Full text Cite

Geometric Validation of Continuous, Finely Sampled 3-D Reconstructions From aOCT and CT in Upper Airway Models.

Journal Article IEEE transactions on medical imaging · April 2019 Identification and treatment of obstructive airway disorders (OADs) are greatly aided by imaging of the geometry of the airway lumen. Anatomical optical coherence tomography (aOCT) is a promising high-speed and minimally invasive endoscopic imaging modalit ... Full text Cite

Combined anatomical optical coherence tomography and intraluminal pressure reveal viscoelasticity of the in vivo airway.

Journal Article Journal of biomedical optics · October 2018 It is hypothesized that the local, viscoelastic (time-dependent) properties of the airway are important to accurately model and ultimately predict dynamic airway collapse in airway obstruction. Toward this end, we present a portable, endoscopic, swept-sour ... Full text Cite

Airway compliance measured by anatomic optical coherence tomography.

Journal Article Biomedical optics express · April 2017 Quantification of airway compliance can aid in the diagnosis and treatment of obstructive airway disorders by detecting regions vulnerable to collapse. Here we evaluate the ability of a swept-source anatomic optical coherence tomography (SSaOCT) system to ... Full text Cite

Multi-modal anatomical Optical Coherence Tomography and CT for in vivo Dynamic Upper Airway Imaging.

Conference Proceedings of SPIE--the International Society for Optical Engineering · February 2017 We describe a novel, multi-modal imaging protocol for validating quantitative dynamic airway imaging performed using anatomical Optical Coherence Tomography (aOCT). The aOCT system consists of a catheter-based aOCT probe that is deployed via a bronchoscope ... Full text Cite

Anatomic optical coherence tomography for dynamic imaging of the upper airway

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · January 1, 2017 To aid in diagnosis and treatment of upper airway obstructive disorders (UAOD), we propose anatomic Optical Coherence Tomography (aOCT) for endoscopic imaging of the upper airway lumen with high speed and resolution. aOCT and CT scans are performed sequent ... Full text Cite

Swept-Source Anatomic Optical Coherence Elastography of Porcine Trachea.

Conference Proceedings of SPIE--the International Society for Optical Engineering · January 2016 Quantitative endoscopic imaging is at the vanguard of novel techniques in the assessment upper airway obstruction. Anatomic optical coherence tomography (aOCT) has the potential to provide the geometry of the airway lumen with high-resolution and in 4 dime ... Full text Cite

Quantitative upper airway endoscopy with swept-source anatomical optical coherence tomography.

Journal Article Biomedical optics express · March 2014 Minimally invasive imaging of upper airway obstructions in children and adults is needed to improve clinical decision-making. Toward this goal, we demonstrate an anatomical optical coherence tomography (aOCT) system delivered via a small-bore, flexible end ... Full text Cite

Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis.

Journal Article Methods (San Diego, Calif.) · February 2012 Noble metal nanoparticles hold great potential as optical contrast agents due to a unique feature, known as the plasmon resonance, which produces enhanced scattering and absorption at specific frequencies. The plasmon resonance also provides a spectral tun ... Full text Cite

Controlled synthesis of gold nanorods and application to brain tumor delineation

Conference Optics InfoBase Conference Papers · December 1, 2011 Gold nanorods are candidates for tumor delineation due to their unique optical properties and biocompatibility. The optical characteristics of nanorods are tuned during synthesis. Anti-EGFR nanorods effectively label tumors within brain slices. © 2011 OSA. ... Cite

Controlled synthesis of gold nanorods and application to brain tumor delineation

Conference Optics InfoBase Conference Papers · January 1, 2011 Gold nanorods are candidates for tumor delineation due to their unique optical properties and biocompatibility. The optical characteristics of nanorods are tuned during synthesis. Anti-EGFR nanorods effectively label tumors within brain slices. © 2011 OSA. ... Full text Cite