John Daniel Shelburne | Scholars@Duke

John Daniel Shelburne
Professor Emeritus of Pathology

Our group has maintained and refined a state of the art analytical electron microscopy laboratory. Research by different members of our group focuses on the following major areas: pathophysiology of the pneumoconioses, cell pathology of laser damage, and correlative microprobe analysis (comparing conventional microprobe analysis with new techniques such as using the free electron laser or FEL).

Current Appointments & Affiliations

Professor Emeritus of Pathology, Pathology, Clinical Science Departments 2008

Contact Information

301M Davison Bldg, Durham, NC 27710
Box 3712 Med Ctr, Durham, NC 27710
john.shelburne@va.gov (919) 286-6907

Background
Education, Training, & Certifications
- Professor, Emeritus, Pathology, Duke University 2008
- Professor, Pathology, Duke University 1985
- Associate Professor, Pathology, Duke University 1978 - 1985
- Assistant Professor, Pathology, Duke University 1973 - 1978
- Resident, Pathology, Duke University 1973 - 1976
- Intern, Pathology, Duke University 1972 - 1973
- M.D., Duke University 1972
- Ph.D., Duke University 1971
Duke Appointment History
- Professor of Pathology, Pathology, Clinical Science Departments 1985 - 2007
- Chairman of Department, Pro Tem, Pathology, Clinical Science Departments 1989 - 1991
Publications & Artistic Works
Selected Publications
- Academic Articles
  - Tucker, J Allan, Alexander Spock, Samuel S. Spicer, John D. Shelburne, and William Bradford. “Inspissation of pancreatic zymogen material in cystic fibrosis.” Ultrastruct Pathol 27, no. 5 (September 2003): 323–35.
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  - Howell, D. N., C. M. Payne, S. E. Miller, and J. D. Shelburne. “Special techniques in diagnostic electron microscopy.” Hum Pathol 29, no. 12 (December 1998): 1339–46. https://doi.org/10.1016/s0046-8177(98)90001-6.
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  - Assalian, A., M. W. Scroggs, M. B. Shields, J. D. Shelburne, A. B. Threlkeld, and E. Worniallo. “Histology of conjunctival vascular endothelium after filtering surgery with mitomycin C in rabbits.” Can J Ophthalmol 31, no. 6 (October 1996): 289–95.
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  - Roggli, V. L., J. W. McDonald, and J. D. Shelburne. “The detection of silicone within tissues.” Arch Pathol Lab Med 118, no. 10 (October 1994): 963–64.
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  - Tucker, J. A., J. D. Shelburne, T. L. Benning, L. Yacoub, and M. Federman. “Filamentous inclusions in acinar cell carcinoma of the pancreas.” Ultrastruct Pathol 18, no. 1–2 (January 1994): 279–86. https://doi.org/10.3109/01913129409016301.
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  - Boyko, O. B., P. C. Burger, J. D. Shelburne, and P. Ingram. “Non-heme mechanisms for T1 shortening: pathologic, CT, and MR elucidation.” Ajnr Am J Neuroradiol 13, no. 5 (September 1992): 1439–45.
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  - McDonagh, D., R. T. Vollmer, and J. D. Shelburne. “Intercellular junctions and tumor behavior in lung cancer.” Mod Pathol 4, no. 4 (July 1991): 436–40.
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  - Benning, T. L., R. T. Vollmer, B. J. Crain, and J. D. Shelburne. “Neuroendocrine carcinoma of the oral cavity.” Mod Pathol 3, no. 5 (September 1990): 631–34.
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  - Dovey, M., C. L. Wisseman, V. L. Roggli, G. M. Roomans, J. D. Shelburne, and A. Spock. “Ultrastructural morphology of the lung in cystic fibrosis.” J Submicrosc Cytol Pathol 21, no. 3 (July 1989): 521–34.
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  - Humphrey, P. A., P. Ingram, A. Tucker, and J. D. Shelburne. “Crystalloids in salivary gland pleomorphic adenomas.” Arch Pathol Lab Med 113, no. 4 (April 1989): 390–93.
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  - Benning, T. L., K. M. McCormack, P. Ingram, D. L. Kaplan, and J. D. Shelburne. “Microprobe analysis of chlorpromazine pigmentation.” Arch Dermatol 124, no. 10 (October 1988): 1541–44.
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  - Mastin, J. P., L. E. Stettler, and J. D. Shelburne. “Quantitative analysis of particulate burden in lung tissue.” Scanning Microsc 2, no. 3 (September 1988): 1613–29.
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  - Tabatowski, K., R. T. Vollmer, J. W. Tello, J. D. Iglehart, J. D. Shelburne, J. Schlom, and W. W. Johnston. “The use of a panel of monoclonal antibodies in ultrastructurally characterized small cell carcinomas of the lung.” Acta Cytol 32, no. 5 (September 1988): 667–74.
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  - Humphrey, P. A., M. W. Scroggs, V. L. Roggli, and J. D. Shelburne. “Pulmonary carcinomas with a sarcomatoid element: an immunocytochemical and ultrastructural analysis.” Hum Pathol 19, no. 2 (February 1988): 155–65. https://doi.org/10.1016/s0046-8177(88)80343-5.
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  - Mastin, J. P., J. D. Shelburne, and L. A. Thet. “Subcellular changes in capillary endothelial cells during repair of hyperoxic lung injury.” J Appl Physiol (1985) 64, no. 2 (February 1988): 689–96. https://doi.org/10.1152/jappl.1988.64.2.689.
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  - Knapp, M. J., V. L. Roggli, J. Kim, J. O. Moore, and J. D. Shelburne. “Pleural amyloidosis.” Arch Pathol Lab Med 112, no. 1 (January 1988): 57–60.
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  - Linder, J., R. B. Stein, V. L. Roggli, R. T. Vollmer, B. P. Croker, R. W. Postlethwait, and J. D. Shelburne. “Polypoid tumor of the esophagus.” Hum Pathol 18, no. 7 (July 1987): 692–700. https://doi.org/10.1016/s0046-8177(87)80240-x.
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  - Roggli, V. L., J. Kolbeck, F. Sanfilippo, and J. D. Shelburne. “Pathology of human mesothelioma. Etiologic and diagnostic considerations.” Pathol Annu 22 Pt 2 (1987): 91–131.
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  - Vollmer, R. T., J. D. Shelburne, and J. D. Iglehart. “Intercellular junctions and tumor stage in small cell carcinoma of the lung.” Hum Pathol 18, no. 1 (January 1987): 22–27. https://doi.org/10.1016/s0046-8177(87)80188-0.
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  - Bigner, D. D., S. H. Bigner, P. C. Burger, J. D. Shelburne, and H. S. Friedman. “Primary brain tumours in Fischer 344 rats chronically exposed to acrylonitrile in their drinking-water.” Food Chem Toxicol 24, no. 2 (February 1986): 129–37. https://doi.org/10.1016/0278-6915(86)90347-9.
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  - Thet, L. A., S. C. Parra, and J. D. Shelburne. “Sequential changes in lung morphology during the repair of acute oxygen-induced lung injury in adult rats.” Exp Lung Res 11, no. 3 (1986): 209–28. https://doi.org/10.3109/01902148609064297.
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  - Iglehart, J. D., W. G. Wolfe, W. B. Vernon, J. D. Shelburne, and D. C. Sabiston. “Electron microscopy in selection of patients with small cell carcinoma of the lung for medical versus surgical therapy.” J Thorac Cardiovasc Surg 90, no. 3 (September 1985): 351–60.
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  - Baker, D., K. G. Kupke, P. Ingram, V. L. Roggli, and J. D. Shelburne. “Microprobe analysis in human pathology.” Scan Electron Microsc, no. Pt 2 (1985): 659–80.
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  - Linder, J., J. D. Shelburne, J. P. Sorge, R. E. Whalen, and D. B. Hackel. “Congenital endodermal heterotopia of the atrioventricular node: evidence for the endodermal origin of so-called mesotheliomas of the atrioventricular node.” Hum Pathol 15, no. 11 (November 1984): 1093–98. https://doi.org/10.1016/s0046-8177(84)80255-5.
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  - Roggli, V. L., P. Ingram, R. W. Linton, W. F. Gutknecht, P. Mastin, and J. D. Shelburne. “New techniques for imaging and analyzing lung tissue.” Environ Health Perspect 56 (June 1984): 163–83. https://doi.org/10.1289/ehp.8456163.
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  - Linton, R. W., M. E. Farmer, P. Ingram, J. R. Sommer, and J. D. Shelburne. “Ultrastructural comparison of ion beam and radiofrequency plasma etching effects on biological tissue sections.” J Microsc 134, no. Pt 1 (April 1984): 101–12. https://doi.org/10.1111/j.1365-2818.1984.tb00508.x.
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  - Lee, J. T., J. D. Shelburne, and J. Linder. “Primary malignant fibrous histiocytoma of the lung. A clinicopathologic and ultrastructural study of five cases.” Cancer 53, no. 5 (March 1, 1984): 1124–30. https://doi.org/10.1002/1097-0142(19840301)53:5<1124::aid-cncr2820530518>3.0.co;2-v.
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  - Walker, S. R., P. Ingram, and J. D. Shelburne. “Energy dispersive x-ray microanalysis of vital dye (erythrosin B) stained cells.” J Microsc 133, no. Pt 2 (February 1984): RP3–4. https://doi.org/10.1111/j.1365-2818.1984.tb00477.x.
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  - Jackson, M. D., R. Albrecht, V. L. Roggli, and J. D. Shelburne. “Pulmonary blastoma: an ultrastructural and histochemical study.” Ultrastruct Pathol 7, no. 4 (1984): 259–68. https://doi.org/10.3109/01913128409141486.
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  - Kornbrust, D. J., T. R. Barfknecht, P. Ingram, and J. D. Shelburne. “Effect of di(2-ethylhexyl) phthalate on DNA repair and lipid peroxidation in rat hepatocytes and on metabolic cooperation in Chinese hamster V-79 cells.” J Toxicol Environ Health 13, no. 1 (1984): 99–116. https://doi.org/10.1080/15287398409530484.
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  - Simel, D. L., J. P. Mastin, P. C. Pratt, C. L. Wisseman, J. D. Shelburne, A. Spock, and P. Ingram. “Scanning electron microscopic study of the airways in normal children and in patients with cystic fibrosis and other lung diseases.” Pediatr Pathol 2, no. 1 (1984): 47–64. https://doi.org/10.3109/15513818409041187.
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  - Thet, L. A., S. C. Parra, and J. D. Shelburne. “Repair of oxygen-induced lung injury in adult rats. The role of ornithine decarboxylase and polyamines.” Am Rev Respir Dis 129, no. 1 (January 1984): 174–81. https://doi.org/10.1164/arrd.1984.129.1.174.
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  - Tatum, A. H., J. D. Shelburne, P. Ingram, D. N. Robertson, H. B. Croxatto, and S. Diaz. “Scanning electron microscopy and X-ray microanalysis of foreign bodies associated with Silastic implants in humans.” Contraception 28, no. 6 (December 1983): 543–52. https://doi.org/10.1016/0010-7824(83)90105-1.
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  - Trimble, J. W., D. S. Mendelson, B. F. Fetter, P. Ingram, J. J. Gallagher, and J. D. Shelburne. “Cutaneous pigmentation secondary to amiodarone therapy.” Arch Dermatol 119, no. 11 (November 1983): 914–18.
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  - Kerr, C. M., J. R. Perfect, P. C. Craven, J. H. Jorgensen, D. J. Drutz, J. D. Shelburne, H. A. Gallis, and R. A. Gutman. “Fungal peritonitis in patients on continuous ambulatory peritoneal dialysis.” Ann Intern Med 99, no. 3 (September 1983): 334–36. https://doi.org/10.7326/0003-4819-99-3-334.
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  - Kupke, K. G., J. P. Pickett, P. Ingram, D. P. Griffis, R. W. Linton, and J. D. Shelburne. “Correlative light, electron, and ion microscopy on a single histologic section.” J Microsc 131, no. Pt 1 (July 1983): RP1–2. https://doi.org/10.1111/j.1365-2818.1983.tb04223.x.
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  - Thet, L. A., D. J. Wrobel, J. D. Crapo, and J. D. Shelburne. “Morphologic aspects of the protection by endotoxin against acute and chronic oxygen-induced lung injury in adult rats.” Lab Invest 48, no. 4 (April 1983): 448–57.
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  - Spicer, S. S., B. A. Schulte, and J. D. Shelburne. “Carbohydrate cytochemistry by transmission and scanning electron microscopy.” Scan Electron Microsc, no. Pt 4 (1983): 1827–34.
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  - deVries, C. R., P. Ingram, S. R. Walker, R. W. Linton, W. F. Gutknecht, and J. D. Shelburne. “Acute toxicity of lead particulates on pulmonary alveolar macrophages. Ultrastructural and microanalytical studies.” Lab Invest 48, no. 1 (January 1983): 35–44.
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  - Hamilton, C. W., J. D. Shelburne, E. H. Bossen, and J. E. Lowe. “A glomus tumor of the jejunum masquerading as a carcinoid tumor.” Hum Pathol 13, no. 9 (September 1982): 859–61. https://doi.org/10.1016/s0046-8177(82)80082-8.
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  - Linton, R. W., M. E. Farmer, P. Ingram, S. R. Walker, and J. D. Shelburne. “Ion beam etching effects in biological microanalysis.” Scan Electron Microsc, no. Pt 3 (1982): 1191–1204.
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  - Farmer, M. E., R. W. Linton, P. Ingram, J. R. Sommer, and J. D. Shelburne. “Preferential sputtering effects in the ion microanalysis of biological tissues.” J Microsc 124, no. Pt 2 (November 1981): RP1–2. https://doi.org/10.1111/j.1365-2818.1981.tb00303.x.
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  - Wisseman, C. L., D. L. Simel, A. Spock, and J. D. Shelburne. “The prevalence of abnormal cilia in normal pediatric lungs.” Arch Pathol Lab Med 105, no. 10 (October 1981): 552–55.
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  - Bowen, J. H., B. H. Woodard, T. K. Barton, P. Ingram, and J. D. Shelburne. “Infantile pulmonary hypertension associated with foreign body vasculitis.” Am J Clin Pathol 75, no. 4 (April 1981): 609–14. https://doi.org/10.1093/ajcp/75.4.609.
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  - Bodner, S. M., P. Ingram, S. S. Spicer, A. Spock, and J. D. Shelburne. “Carbohydrate histochemistry of surface epithelium of rat trachea: backscatter and x-ray imaging.” Scan Electron Microsc, no. Pt 2 (1981): 105–14.
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  - Bowen, J. H., B. H. Woodward, J. A. Mossler, P. Ingram, and J. D. Shelburne. “Energy dispersive x-ray detection of thorium dioxide.” Arch Pathol Lab Med 104, no. 9 (September 1980): 459–61.
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  - Ruth, W. K., J. D. Shelburne, and B. V. Jegasothy. “Zosteriform lentiginous nevus.” Arch Dermatol 116, no. 4 (April 1980): 478.
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  - Ingram, P., and J. D. Shelburne. “Total rate imaging with x-rays (TRIX)--a simple method of forming a non-projection x-ray image in the SEM using an energy dispersive detector and its application to biological specimens.” Scan Electron Microsc, no. Pt 2 (1980): 285–95.
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  - Linton, R. W., S. R. Walker, C. R. DeVries, P. Ingram, and J. D. Shelburne. “Ion microanalysis of cells.” Scan Electron Microsc, no. Pt 2 (1980): 583–96.
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  - Feldman, J. M., J. A. Blalock, R. T. Zern, J. D. Shelburne, J. T. Gaede, R. E. Farrell, and S. A. Wells. “Deficiency of dopamine-beta-hydroxylase. A new mechanism for normotensive pheochromocytomas.” Am J Clin Pathol 72, no. 2 (August 1979): 175–85. https://doi.org/10.1093/ajcp/72.2.175.
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  - Scullin, D. C., J. D. Shelburne, and H. J. Cohen. “Pseudo-Gaucher cells in multiple myeloma.” Am J Med 67, no. 2 (August 1979): 347–52. https://doi.org/10.1016/0002-9343(79)90413-3.
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  - Kariman, K., J. D. Shelburne, W. Gough, M. J. Zacheck, and J. A. Blackmon. “Pathologic findings and long-term sequelae in Legionnaires' disease.” Chest 75, no. 6 (June 1979): 736–39. https://doi.org/10.1378/chest.75.6.736.
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  - Feussner, J. R., J. D. Shelburne, S. Bredehoeft, and H. J. Cohen. “Arsenic-induced bone marrow toxicity: ultrastructural and electron-probe analysis.” Blood 53, no. 5 (May 1979): 820–27.
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  - Pratt, P. C., R. T. Vollmer, J. D. Shelburne, and J. D. Crapo. “Pulmonary morphology in a multihospital collaborative extracorporeal membrane oxygenation project. I. Light microscopy.” Am J Pathol 95, no. 1 (April 1979): 191–214.
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  - Bell, S. W., S. K. Masters, P. Ingram, M. Waters, and J. D. Shelburne. “Ultrastructure and x-ray microanalysis of macrophages exposed to cadmium chloride.” Scan Electron Microsc, no. 3 (1979): 111–21.
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  - Masters, S. K., S. W. Bell, P. Ingram, D. O. Adams, and J. D. Shelburne. “Preparative techniques for freezing and freeze-sectioning macrophages for energy dispersive x-ray microanalysis.” Scan Electron Microsc, no. 3 (1979): 97–122.
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  - Matthews, W. H., R. H. Balzer, J. D. Shelburne, P. C. Pratt, and J. A. Kylstra. “Steady-state gas exchange in normothermic, anesthetized, liquid-ventilated dogs.” Undersea Biomed Res 5, no. 4 (December 1978): 341–54.
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  - Mansbach, C. M., J. D. Shelburne, R. D. Stevens, and W. O. Dobbins. “Lymph-node bacilliform bodies resembling those of Whipple's disease in a patient without intestinal involvement.” Ann Intern Med 89, no. 1 (July 1978): 64–66. https://doi.org/10.7326/0003-4819-89-1-64.
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  - Copeland, D. D., S. W. Bell, and J. D. Shelburne. “Hemidesmosome-like intercellular specializations in human meningiomas.” Cancer 41, no. 6 (June 1978): 2242–49. https://doi.org/10.1002/1097-0142(197806)41:6<2242::aid-cncr2820410625>3.0.co;2-u.
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  - Gaede, J. T., R. W. Postlethwait, J. D. Shelburne, J. L. Cox, and W. F. Hamilton. “Leiomyosarcoma of the esophagus. Report of two cases, one with associated squamous cell carcinoma.” J Thorac Cardiovasc Surg 75, no. 5 (May 1978): 740–46.
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  - Woodard, B. H., J. D. Shelburne, R. T. Vollmer, and R. W. Postlethwait. “Mucoepidermoid carcinoma of the esophagus: a case report.” Hum Pathol 9, no. 3 (May 1978): 352–54. https://doi.org/10.1016/s0046-8177(78)80093-8.
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  - Runge, P. M., J. N. Schwartz, H. F. Seigler, B. H. Woodard, and J. D. Shelburne. “Gallbladder with ectopic gastric mucosa: ultrastructural analysis.” Arch Pathol Lab Med 102, no. 4 (April 1978): 209–11.
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  - Michel, R. G., B. H. Woodard, J. D. Shelburne, and E. H. Bossen. “Ceruminous gland adenocarcinoma: a light and electron microscopic study.” Cancer 41, no. 2 (February 1978): 545–53. https://doi.org/10.1002/1097-0142(197802)41:2<545::aid-cncr2820410222>3.0.co;2-w.
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  - Gutman, R. A., J. D. Shelburne, N. J. Peterson, L. A. Carson, and M. S. Favero. “Aseptic peritonitis with home peritoneal dialysis.” N Engl J Med 297, no. 14 (October 6, 1977): 788. https://doi.org/10.1056/NEJM197710062971415.
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  - Shields, M. B., J. D. Shelburne, and S. W. Bell. “The ultrastructure of human limbal collagen.” Invest Ophthalmol Vis Sci 16, no. 9 (September 1977): 864–66.
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  - Shields, M. B., M. J. Bradbury, J. D. Shelburne, and S. W. Bell. “The permeability of the outer layers of limbus and anterior sclera.” Invest Ophthalmol Vis Sci 16, no. 9 (September 1977): 866–69.
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  - Shields, M. B., R. C. McCoy, and J. D. Shelburne. “Immunofluorescent studies on the trabecular meshwork in open-angle glaucoma.” Invest Ophthalmol 15, no. 12 (December 1976): 1014–17.
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  - Gold, J. H., J. D. Shelburne, and E. H. Bossen. “Meningeal mycosis fungoides: cytologic and ultrastructural aspects.” Acta Cytol 20, no. 4 (July 1976): 349–55.
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  - Mitchener, J. S., J. D. Shelburne, W. D. Bradford, and H. K. Hawkins. “Cellular autophagocytosis induced by deprivation of serum and amino acids in HeLa cells.” Am J Pathol 83, no. 3 (June 1976): 485–92.
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  - Wells, S. A., J. C. Gunnells, J. D. Shelburne, A. B. Schneider, and L. M. Sherwood. “Transplantation of the parathyroid glands in man: clinical indications and results.” Surgery 78, no. 1 (July 1975): 34–44.
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  - Postlethwait, R. W., A. S. Wechsler, and J. D. Shelburne. “Pseudosarcoma of the esophagus.” Ann Thorac Surg 19, no. 2 (February 1975): 198–205. https://doi.org/10.1016/s0003-4975(10)64003-9.
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  - Bossen, E. H., J. D. Shelburne, and B. S. Verkauf. “Respiratory muscle involvement in infantile myotonic dystrophy.” Arch Pathol 97, no. 4 (April 1974): 250–52.
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  - Shelburne, J. D., A. U. Arstila, and B. F. Trump. “Studies on cellular autophagocytosis. The relationship of autophagocytosis to protein synthesis and to energy metabolism in rat liver and flounder kidney tubules in vitro.” Am J Pathol 73, no. 3 (December 1973): 641–70.
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  - Shelburne, J. D., A. U. Arstila, and B. F. Trump. “Studies on cellular autophagocytosis. Cyclic AMP- and dibutyryl cyclic AMP-stimulated autophagy in rat liver.” Am J Pathol 72, no. 3 (September 1973): 521–40.
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  - Arstila, A. U., J. D. Shelburne, and B. F. Trump. “Studies on cellular autophagocytosis. A histochemical study on sequential alterations of mitochondria in the glucagon-induced autophagic vacuoles of rat liver.” Lab Invest 27, no. 3 (September 1972): 317–23.
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  - Laiho, K. U., J. D. Shelburne, and B. F. Trump. “Observations on cell volume, ultrastructure, mitochondrial conformation and vital-dye uptake in Ehrlich ascites tumor cells. Effects of inhibiting energy production and function of the plasma membrane.” Am J Pathol 65, no. 1 (October 1971): 203–30.
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  - Ginn, F. L., J. D. Shelburne, and B. F. Trump. “Disorders of cell volume regulation. I. Effects of inhibition of plasma membrane adenosine triphosphatase with ouabain.” Am J Pathol 53, no. 6 (December 1968): 1041–71.
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