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John Daniel Shelburne

Professor Emeritus of Pathology
Pathology
Box 3712 Med Ctr, Durham, NC 27710
301M Davison Bldg, Durham, NC 27710

Selected Publications


Inspissation of pancreatic zymogen material in cystic fibrosis.

Journal Article Ultrastruct Pathol · 2003 Cystic fibrosis is characterized by the elaboration of abnormal, thick, tenacious mucus resulting in obstructive disease in sites such as the lung and pancreas. In the pancreas, acinar plugs of mucus have been reported as the earliest recognizable morpholo ... Link to item Cite

Special techniques in diagnostic electron microscopy.

Journal Article Hum Pathol · December 1998 The power of electron microscopy as a diagnostic tool can be amplified considerably by the application of ancillary preparative and analytic methods. Subcellular chemistry and structure can be examined by various forms of microprobe analysis and by special ... Full text Link to item Cite

Histology of conjunctival vascular endothelium after filtering surgery with mitomycin C in rabbits.

Journal Article Can J Ophthalmol · October 1996 OBJECTIVE: To examine histologic changes in conjunctival vasculature during the first 72 hours following filtering surgery with adjunctive mitomycin C in rabbits. DESIGN: Thirty-six New Zealand white rabbits underwent unilateral posterior lip sclerectomy. ... Link to item Cite

The detection of silicone within tissues.

Journal Article Arch Pathol Lab Med · October 1994 Link to item Cite

Filamentous inclusions in acinar cell carcinoma of the pancreas.

Journal Article Ultrastruct Pathol · 1994 Acinar cell carcinoma of the pancreas exhibits a spectrum of histologic appearances. Some tumors can be readily identified by light microscopy, but others resemble endocrine/neuroendocrine neoplasms. Ultrastructurally, though large zymogen granules of acin ... Full text Link to item Cite

Non-heme mechanisms for T1 shortening: pathologic, CT, and MR elucidation.

Journal Article AJNR Am J Neuroradiol · 1992 PURPOSE: To further elucidate the nonparamagnetic effects of T1-relaxation mechanisms in MR imaging. PATIENTS AND METHODS: In 12 patients with lesions having hyperintense signal on T1-weighted spin-echo MR, findings were correlated with autopsy/surgical bi ... Link to item Cite

Intercellular junctions and tumor behavior in lung cancer.

Journal Article Mod Pathol · July 1991 We examined the ultrastructure of 76 lung cancers and counted the number of intercellular junctions per area of tissue section. The total number of desmosomes, intermediate junctions, and tight junctions ranged from zero to 300 per 0.157 cm2 of tissue sect ... Link to item Cite

Neuroendocrine carcinoma of the oral cavity.

Journal Article Mod Pathol · September 1990 We report the clinical and pathologic features of a small cell neuroendocrine carcinoma arising in the oral cavity of a 63-year-old man. The tumor stained positively with neuron-specific enolase and had dense core granules ultrastructurally. Chemotherapy o ... Link to item Cite

Ultrastructural morphology of the lung in cystic fibrosis.

Journal Article J Submicrosc Cytol Pathol · July 1989 Cystic fibrosis (CF) is the most common lethal genetic disease among Caucasians, with much of the morbidity and most of the mortality related to pulmonary complications. The underlying defect in this disease has yet to be precisely defined, so it is somewh ... Link to item Cite

Crystalloids in salivary gland pleomorphic adenomas.

Journal Article Arch Pathol Lab Med · April 1989 Two types of crystalloids in salivary gland pleomorphic adenomas were studied by light microscopy and electron microscopy. The first type of crystalloid, the previously described tyrosine-rich crystalloid, was identified in three (1.5%) of 205 cases. The c ... Link to item Cite

Microprobe analysis of chlorpromazine pigmentation.

Journal Article Arch Dermatol · October 1988 We describe the histochemical, ultrastructural, and microanalytical features of a skin biopsy specimen obtained from a patient with chlorpromazine pigmentation. Golden-brown pigment granules were present in the dermis, predominantly in a perivascular arran ... Link to item Cite

Quantitative analysis of particulate burden in lung tissue.

Journal Article Scanning Microsc · September 1988 Numerous methods have been used in the preparation and analysis of the particulate matter deposited in human lungs. Preparation techniques include those for particle isolation and for in situ analysis. Analytical techniques include bulk and particle-by-par ... Link to item Cite

Subcellular changes in capillary endothelial cells during repair of hyperoxic lung injury.

Journal Article J Appl Physiol (1985) · February 1988 We studied the changes in subcellular ultrastructure associated with the hypertrophy of capillary endothelial cells during repair of hyperoxic (100% O2) lung injury in rats. We used stereologic-morphometric measurements at different magnifications to deter ... Full text Link to item Cite

Pulmonary carcinomas with a sarcomatoid element: an immunocytochemical and ultrastructural analysis.

Journal Article Hum Pathol · February 1988 Eight primary carcinomas of the lung with a prominent spindle-cell sarcomatoid component were studied by immunocytochemical staining and electron microscopy. The eight tumors were indistinguishable by conventional light microscopy, with the exception of on ... Full text Link to item Cite

The use of a panel of monoclonal antibodies in ultrastructurally characterized small cell carcinomas of the lung.

Journal Article Acta Cytol · 1988 In recent electron microscopic studies of 51 small cell carcinomas of the lung, the ultrastructural features of epithelial differentiation, particularly the presence of desmosomes, were associated with a tendency toward localized disease, clinical resectab ... Link to item Cite

Pleural amyloidosis.

Journal Article Arch Pathol Lab Med · January 1988 Pleural amyloidosis has been reported rarely, and the diagnosis of this disease by Cope needle biopsy has, to our knowledge, been reported only once previously. We report two patients in whom the diagnosis of pleural amyloidosis was made by biopsy specimen ... Link to item Cite

Polypoid tumor of the esophagus.

Journal Article Hum Pathol · July 1987 Five cases of an uncommon esophageal tumor consisting of a mucosal squamous cell carcinoma that surrounds a polypoid mass of spindle cells were examined. The spindle cell component was composed of elongated cells with blunt nuclei, admixed with multinuclea ... Full text Link to item Cite

Intercellular junctions and tumor stage in small cell carcinoma of the lung.

Journal Article Hum Pathol · January 1987 The authors have studied the ultrastructural features of 52 cases of oat cell carcinoma of the lung and have related their observations to tumor stage and patient survival. Only the type of cell junctions seems to be of prognostic importance. Tumors with i ... Full text Link to item Cite

Primary brain tumours in Fischer 344 rats chronically exposed to acrylonitrile in their drinking-water.

Journal Article Food Chem Toxicol · February 1986 Acrylonitrile (ACN) has been tested for carcinogenicity by various routes in a number of rat strains. At relatively high levels of administration (e.g. 500 ppm in the drinking-water) there were statistically significant increases in microscopically detecta ... Full text Link to item Cite

Sequential changes in lung morphology during the repair of acute oxygen-induced lung injury in adult rats.

Journal Article Exp Lung Res · 1986 We studied changes in lung ultrastructure and collagen content during the repair of acute lung injury in adult rats exposed to 100% O2 for 60 h and recovering in ambient air. In the interstitium, during the first 3 days of repair, the number of neutrophils ... Full text Link to item Cite

Electron microscopy in selection of patients with small cell carcinoma of the lung for medical versus surgical therapy.

Journal Article J Thorac Cardiovasc Surg · September 1985 Fifty-one cases of small cell carcinoma of the lung were studied by electron microscopy in order to determine if ultrastructural subsets could be found and if these subsets predicted clinical behavior. All of these cases were considered bona fide small cel ... Link to item Cite

Microprobe analysis in human pathology.

Journal Article Scan Electron Microsc · 1985 This tutorial paper reviews the literature on the application of microprobe analysis to practical problems in diagnostic human pathology. The goal is to allow the reader ready access to the literature on specific clinical problems. Specimen preparation and ... Link to item Cite

Congenital endodermal heterotopia of the atrioventricular node: evidence for the endodermal origin of so-called mesotheliomas of the atrioventricular node.

Journal Article Hum Pathol · November 1984 A case of so-called mesothelioma of the atrioventricular node is presented. Controversy exists as to whether this lesion is of mesodermal or endodermal origin. The light and electron microscopic morphologic characteristics in this case were identical to th ... Full text Link to item Cite

New techniques for imaging and analyzing lung tissue.

Journal Article Environ Health Perspect · June 1984 The recent technological revolution in the field of imaging techniques has provided pathologists and toxicologists with an expanding repertoire of analytical techniques for studying the interaction between the lung and the various exogenous materials to wh ... Full text Link to item Cite

Ultrastructural comparison of ion beam and radiofrequency plasma etching effects on biological tissue sections.

Journal Article J Microsc · April 1984 Three dry etching techniques (Ar+ ion beam, O2+ ion beam, O2 radiofrequency electrodeless discharge) were compared with respect to preferential etching and damage to the ultrastructure of glutaraldehyde-fixed Epon-embedded frog skeletal muscle sections. SE ... Full text Link to item Cite

Primary malignant fibrous histiocytoma of the lung. A clinicopathologic and ultrastructural study of five cases.

Journal Article Cancer · March 1, 1984 Primary sarcomas of the lung are rare malignant neoplasms which have usually been classified as fibrosarcomas or as leiomyosarcomas. Only five cases of primary malignant fibrous histiocytoma (MFH) of the lung have previously been reported in the literature ... Full text Link to item Cite

Effect of di(2-ethylhexyl) phthalate on DNA repair and lipid peroxidation in rat hepatocytes and on metabolic cooperation in Chinese hamster V-79 cells.

Journal Article J Toxicol Environ Health · 1984 Experiments were conducted to test the hypothesis that the hepatocarcinogenicity of di(2-ethylhexyl) phthalate (DEHP) is due to its ability to produce DNA damage, either directly or as a result of the proliferation of peroxisomes and accompanying increased ... Full text Link to item Cite

Repair of oxygen-induced lung injury in adult rats. The role of ornithine decarboxylase and polyamines.

Journal Article Am Rev Respir Dis · January 1984 We studied the repair of lung injury in adult rats exposed to 100% oxygen for 60 h, then placed in ambient air. Lung ornithine decarboxylase (ODC) activity and polyamine (putrescine, spermidine, and spermine) content during repair were correlated with chan ... Full text Link to item Cite

Scanning electron microscopic study of the airways in normal children and in patients with cystic fibrosis and other lung diseases.

Journal Article Pediatr Pathol · 1984 Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine pulmonary tissue from 9 patients with cystic fibrosis (CF), 12 patients with diseases other than CF, and from two surgically resected specimens with no known ... Full text Link to item Cite

Pulmonary blastoma: an ultrastructural and histochemical study.

Journal Article Ultrastruct Pathol · 1984 We present a unique case of pulmonary blastoma and describe its histology and ultrastructure. The stromal component exhibited bizarre multinucleated giant cells containing previously undescribed granules. These membrane-limited granules were eosinophilic, ... Full text Link to item Cite

Scanning electron microscopy and X-ray microanalysis of foreign bodies associated with Silastic implants in humans.

Journal Article Contraception · December 1983 Polydimethylsiloxane (Silastic) capsules containing megestrol acetate have been implanted in subcutaneous tissue as a method of long-term contraception. Histological studies revealed a granulomatous foreign body reaction around these capsules with birefrin ... Full text Link to item Cite

Cutaneous pigmentation secondary to amiodarone therapy.

Journal Article Arch Dermatol · November 1983 Amiodarone (Cordarone) is an iodinated cardiac antiarrhythmic drug that causes a slate-gray discoloration of the sun-exposed skin and a yellow-brown stippling of the cornea. Histopathologically, biopsy specimens of aminodarone pigmentation sites disclose y ... Link to item Cite

Fungal peritonitis in patients on continuous ambulatory peritoneal dialysis.

Journal Article Ann Intern Med · September 1983 Fungal peritonitis is a rare complication in patients on continuous ambulatory peritoneal dialysis. We report five recent cases and their management. The fungi isolated were Candida albicans, C. parapsilosis, Exophiala jeanselmei, Drechslera spicifera, and ... Full text Link to item Cite

Morphologic aspects of the protection by endotoxin against acute and chronic oxygen-induced lung injury in adult rats.

Journal Article Lab Invest · April 1983 Small doses of endotoxin are reported to protect against O2-induced lung injury in rats. To better understand the cellular basis of this effect, we morphometrically analyzed the extent to which endotoxin modified the changes in lung ultrastructure occurrin ... Link to item Cite

Carbohydrate cytochemistry by transmission and scanning electron microscopy.

Journal Article Scan Electron Microsc · 1983 Macromolecules containing abundant carbohydrate moieties occur widely distributed on cell surfaces and in cell organelles. Extension to the ultrastructural level of light microscopic cytochemical methods for demonstrating the complex carbohydrates has prov ... Link to item Cite

Acute toxicity of lead particulates on pulmonary alveolar macrophages. Ultrastructural and microanalytical studies.

Journal Article Lab Invest · January 1983 Although it is well established that respiratory uptake of lead-containing particles plays a substantial role in the epidemiology of plumbism, relatively little is known about the role of the pulmonary alveolar macrophage in lead poisoning. An in vitro sys ... Link to item Cite

Ion beam etching effects in biological microanalysis.

Journal Article Scan Electron Microsc · 1982 Oxygen ion beam sputter etching used in SIMS has been shown to produce morphologic effects which have similarities and differences in comparison to rf plasma etching of biological specimens. For example, selective retention of nuclear and plasma membranes ... Link to item Cite

The prevalence of abnormal cilia in normal pediatric lungs.

Journal Article Arch Pathol Lab Med · October 1981 Abnormal respiratory cells have been reported in cases of chronic pediatric respiratory infection. In some cases, there is a specific defect of most cilia, but in others there is a variety of derangements of the architecture of only a fraction of the cilia ... Link to item Cite

Infantile pulmonary hypertension associated with foreign body vasculitis.

Journal Article Am J Clin Pathol · April 1981 An infant dying with pulmonary hypertension had a pulmonary vessel foreign body vasculitis as identified by light microscopy and characterized ultrastructurally by scanning electron microscopy and energy-dispersive x-ray analysis. The inclusions were of tw ... Full text Link to item Cite

Energy dispersive x-ray detection of thorium dioxide.

Journal Article Arch Pathol Lab Med · September 1980 Since the recognition of the development of certain malignant neoplasms in association with thorium dioxide (Thorotrast), its presence has been documented by light microscopic appearance and time-consuming autoradiography. Energy dispersive x-ray microanal ... Link to item Cite

Zosteriform lentiginous nevus.

Journal Article Arch Dermatol · April 1980 Link to item Cite

Ion microanalysis of cells.

Journal Article Scan Electron Microsc · 1980 The application of ion microanalysis (IMA) to the chemical characterization of freeze-fixed, freeze-dried cells is reviewed. Particular emphasis is given to pathological studies involving the determination of the chemical composition of isolated cells (e.g ... Link to item Cite

Total rate imaging with x-rays (TRIX)--a simple method of forming a non-projection x-ray image in the SEM using an energy dispersive detector and its application to biological specimens.

Journal Article Scan Electron Microsc · 1980 X-ray images can be formed in a conventional scanning electron microscope equipped with a Si(Li) energy dispersive spectrometer. All the x-ray events generated in the electron beam scanning process are synchronously displayed in the same manner as for dot ... Link to item Cite

Pseudo-Gaucher cells in multiple myeloma.

Journal Article Am J Med · August 1979 A bone biopsy specimen from a patient with multiple myeloma showed numerous Gaucher-like cells scattered throughout a homogeneous background of plasma cells. Further studies using histochemical stains, immunofluorescence, and light and electron microscopy ... Full text Link to item Cite

Pathologic findings and long-term sequelae in Legionnaires' disease.

Journal Article Chest · June 1979 A recently recognized sporadic case of Legionnaires' disease occurring in North Carolina is described. Diagnosis was made by special serologic studies performed at the Center for Disease Control, Atlanta. The light-microscopic and electron-microscopic find ... Full text Link to item Cite

Arsenic-induced bone marrow toxicity: ultrastructural and electron-probe analysis.

Journal Article Blood · May 1979 A patient with severe arsenic poisoning that resulted in marked peripheral blood and bone marrow abnormalities, including megaloblastic erythropoiesis experienced many of the previously reported hematologic complications of arsenic poisoning: leukopenia, g ... Link to item Cite

Pulmonary morphology in a multihospital collaborative extracorporeal membrane oxygenation project. I. Light microscopy.

Journal Article Am J Pathol · April 1979 This report presents the light microscopic morphology found at autopsy in 59 patients who participated in an organized controlled trial of extracorporeal oxygenation as therapy for acute respiratory failure. Observations were recorded as objectively as pos ... Link to item Cite

Preparative techniques for freezing and freeze-sectioning macrophages for energy dispersive x-ray microanalysis.

Journal Article Scan Electron Microsc · 1979 In order to study the subcellular distribution of normal intracellular electrolytes and of metal pollutants, rabbit alveolar macrophages and mouse peritoneal macrophages were maintained in standard tissue culture medium with or without various concentratio ... Link to item Cite

Ultrastructure and x-ray microanalysis of macrophages exposed to cadmium chloride.

Journal Article Scan Electron Microsc · 1979 Macrophages have a direct role in the inflammatory response to cadmium exposure. Cadmium is not only an important air pollutant, but is also one component of cigarette smoke. To study the effects of soluble cadmium on macrophages, two model systems were ch ... Link to item Cite

Steady-state gas exchange in normothermic, anesthetized, liquid-ventilated dogs.

Journal Article Undersea Biomed Res · December 1978 Anesthetized, paralyzed, purebred beagle dogs were ventilated for 45 min (n = 5) and 60 min (n = 5) with oxygenated (PIO2 = 685 mmHg) FC-80 fluorocarbon liquid at 38 degrees C. The PaCO2 remained constant at approximately 43 mmHg during 60 min of liquid ve ... Link to item Cite

Lymph-node bacilliform bodies resembling those of Whipple's disease in a patient without intestinal involvement.

Journal Article Ann Intern Med · July 1978 A 38-year-old man developed symptoms of arthalgias and arthritis, lymphadenopathy, and weight loss. An axillary lymph-node biopsy was done in the diagnostic study; a periodic acid Schiff stain, done for evidence of fungal infection, showed periodic acid Sc ... Full text Link to item Cite

Hemidesmosome-like intercellular specializations in human meningiomas.

Journal Article Cancer · June 1978 Desmosomes, gap junctions and tight junctions are intercellular specializations which have been described in human meningiomas. We report, in five of five meningiomas, including a pulmonary metastasis from a malignant intracranial meningioma, a fourth type ... Full text Link to item Cite

Mucoepidermoid carcinoma of the esophagus: a case report.

Journal Article Hum Pathol · May 1978 The eighth reported case of mucoepidermoid carcinoma of the esophagus is described by light microscopic and ultrastructural examination. The reason of such a paucity of reports, the probable origin, and the natural history of this neoplasm are examined thr ... Full text Link to item Cite

Gallbladder with ectopic gastric mucosa: ultrastructural analysis.

Journal Article Arch Pathol Lab Med · April 1978 The clinical and pathological findings of two patients with ectopic gastric mucosa within the gallbladder are studied and compared with the findings recorded in the literature. A contrast between incidental and symptomatic ectopia was noted in these cases. ... Link to item Cite

Ceruminous gland adenocarcinoma: a light and electron microscopic study.

Journal Article Cancer · February 1978 Tumors of ceruminous gland origin in the external auditory canal are rare in man. A case is described in which such a tumor presented as an invasive vascular temporal bone neoplasm, mimicking a glomus jugulare tumor. Light and electron microscopic study of ... Full text Link to item Cite

Aseptic peritonitis with home peritoneal dialysis.

Journal Article N Engl J Med · October 6, 1977 Full text Link to item Cite

The ultrastructure of human limbal collagen.

Journal Article Invest Ophthalmol Vis Sci · September 1977 In human limbal tissue from four glaucomatous and four nonglaucomatous eyes, the average collagen fibril diameter in the inner and outer layers was 100.86 and 115.33 nm., respectively. In both layers the fibrils occupied an average of two thirds of the tot ... Link to item Cite

The permeability of the outer layers of limbus and anterior sclera.

Journal Article Invest Ophthalmol Vis Sci · September 1977 An in vitro study suggests that flow through the scleral flap of a trabeculectomy procedure can significantly increase outflow. It also suggests that this flow may be through vessels in the flap or through the ground substance of the flap. ... Link to item Cite

Immunofluorescent studies on the trabecular meshwork in open-angle glaucoma.

Journal Article Invest Ophthalmol · December 1976 The trabecular meshwork of eyes with open-angle glaucoma has been demonstrated to have an increase in gamma globulin and plasma cells, raising the question of an immunogenic mechanism in this disorder. In the present study, however, immunofluorescence assa ... Link to item Cite

Cellular autophagocytosis induced by deprivation of serum and amino acids in HeLa cells.

Journal Article Am J Pathol · June 1976 Morphologic alterations in HeLa cells deprived of serum and amino acids were studied by light and electron microscopy. After complete deprivation for 1, 3, or 6 hours, many cells contained autophagic vacuoles enclosing clearly recognizable cell organelles. ... Link to item Cite

Meningeal mycosis fungoides: cytologic and ultrastructural aspects.

Journal Article Acta Cytol · 1976 Mycosis cells were identified in the pre-morbid cerebrospinal fluid of a patient with neurological symptoms and mycosis fungoides (MF). Light and electron microscopic examination at autopsy confirmed leptomeningeal involvement by mycosis fungoides. The cel ... Link to item Cite

Transplantation of the parathyroid glands in man: clinical indications and results.

Journal Article Surgery · July 1975 The physiologic function of human parathyroid autografts and allografts has not been demonstrated conclusively. During the past 30 months, we have transplanted parathyroid glands in 29 patients and tested their functional status. One immunosuppressed apara ... Link to item Cite

Pseudosarcoma of the esophagus.

Journal Article Ann Thorac Surg · February 1975 Polypoid lesions of the esophagus occur infrequently and may be benign or malignant. One malignant polypoid tumor, pseudosarcoma, appears to be a distinct pathological entity; the polypoid portion is composed of sarcomatous spindle cells and the base of th ... Full text Link to item Cite

Studies on cellular autophagocytosis. The relationship of autophagocytosis to protein synthesis and to energy metabolism in rat liver and flounder kidney tubules in vitro.

Journal Article Am J Pathol · December 1973 The purpose of the present study was to elucidate the metabolic requirements of autophagocytosis. Two model systems were used for this purpose: a) glucagon-induced autophagocytosis in the rat liver, and b) the wave of autophagocytosis which occurs when iso ... Link to item Cite

Studies on cellular autophagocytosis. Cyclic AMP- and dibutyryl cyclic AMP-stimulated autophagy in rat liver.

Journal Article Am J Pathol · September 1973 Intraperitoneal injections of either cyclic AMP or dibutyryl cyclic AMP are capable of producing a wave of autophagy in rat liver which resembles closely that stimulated by glucagon. These autophagic vacuoles are acid-phosphatase-positive and appear to ari ... Link to item Cite

Observations on cell volume, ultrastructure, mitochondrial conformation and vital-dye uptake in Ehrlich ascites tumor cells. Effects of inhibiting energy production and function of the plasma membrane.

Journal Article Am J Pathol · October 1971 The ultrastructure, volume, vital-dye uptake, ATP levels and amounts of intracelular water have been studied in Ehrlich ascites cells treated with two classes of inhibitors. Cell membrane injury with 10(-3) M parachloromercuribenzene sulfonate (PCMBS) resu ... Link to item Cite