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Ion beam etching effects in biological microanalysis.

Publication ,  Journal Article
Linton, RW; Farmer, ME; Ingram, P; Walker, SR; Shelburne, JD
Published in: Scan Electron Microsc
1982

Oxygen ion beam sputter etching used in SIMS has been shown to produce morphologic effects which have similarities and differences in comparison to rf plasma etching of biological specimens. For example, selective retention of nuclear and plasma membranes is observed in both cases, however, heterochromatin is preferentially sputtered by O2+ bombardment in SIMS and preferentially preserved during O2 plasma etching. Sputter yield variations resulting from structural microheterogeneity are illustrated (e.g. etch-resistant nucleoli in preferentially etched nuclei), including their impact upon ion image formation in an ion microanalyzer. These image artifacts must be evaluated before secondary ion images revealing subcellular organelles can be related quantitatively to elemental localization in cells or tissues. To minimize gross surface roughness effects such as cone formation induced by ion bombardment, ion imaging studies of thin sections using low primary ion doses are indicated.

Duke Scholars

Published In

Scan Electron Microsc

ISSN

0586-5581

Publication Date

1982

Issue

Pt 3

Start / End Page

1191 / 1204

Location

United States

Related Subject Headings

  • Tantalum
  • Sodium
  • Rabbits
  • Potassium
  • Oxygen
  • Microscopy, Electron, Scanning
  • Mass Spectrometry
  • Ions
  • Intracellular Membranes
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Linton, R. W., Farmer, M. E., Ingram, P., Walker, S. R., & Shelburne, J. D. (1982). Ion beam etching effects in biological microanalysis. Scan Electron Microsc, (Pt 3), 1191–1204.
Linton, R. W., M. E. Farmer, P. Ingram, S. R. Walker, and J. D. Shelburne. “Ion beam etching effects in biological microanalysis.Scan Electron Microsc, no. Pt 3 (1982): 1191–1204.
Linton RW, Farmer ME, Ingram P, Walker SR, Shelburne JD. Ion beam etching effects in biological microanalysis. Scan Electron Microsc. 1982;(Pt 3):1191–204.
Linton, R. W., et al. “Ion beam etching effects in biological microanalysis.Scan Electron Microsc, no. Pt 3, 1982, pp. 1191–204.
Linton RW, Farmer ME, Ingram P, Walker SR, Shelburne JD. Ion beam etching effects in biological microanalysis. Scan Electron Microsc. 1982;(Pt 3):1191–1204.

Published In

Scan Electron Microsc

ISSN

0586-5581

Publication Date

1982

Issue

Pt 3

Start / End Page

1191 / 1204

Location

United States

Related Subject Headings

  • Tantalum
  • Sodium
  • Rabbits
  • Potassium
  • Oxygen
  • Microscopy, Electron, Scanning
  • Mass Spectrometry
  • Ions
  • Intracellular Membranes
  • Humans