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John Griffith Toffaletti

Professor in Pathology
Pathology
Duke Box 3015, Durham, NC 27710
DUHS Central Automated Lab Rm, Durham, NC 27705

Overview


Research Abstract

Use of lactate measurements as indicator of inadequate oxygen utilization and/or mitochondrial dysfunction in critical care. Blood lactate measurements are correlated to clinical progress of patients with sepsis, during and after surgery, during ECMO, and for triage use in the Emergency Department.

Effects of changes in ionized calcium and magnesium on concentrations of parathyroid hormone (PTH) in blood. Normal blood donors are given an infusion of a physiologic solution after donating blood, which should alter free calcium and/or magnesium to help determine the relative effects of calcium and magnesium on PTH secretion.

The changes and variation over time of serum creatinine, cystatin C, eGFR, and GFR measurements in healthy persons and how this relates to diagnosing changes in renal function.

Effects of albumin and other colloids on hematocrit measured by impedance. Blood from patients on cardiopulmonary bypass often shows lower hematocrit results by impedance compared to centrifugation. We have examined the effects of albumin and Hespan (Hetastarch) on impedance-measured hematocrit on different analyzers.

The effects on pO2 of transport by pneumatic tube. Increases in pO2 have been noted if blood contains even tiny air bubbles in the syringe when sent by pneumatic transport.

Current Appointments & Affiliations


Professor in Pathology · 2006 - Present Pathology, Clinical Science Departments

Recent Publications


A 36-Year-Old Woman With Intermittent Cyanosis.

Journal Article Chest · August 2024 A 36-year-old woman with a medical history of opioid use disorder and frequent urinary tract infections presented to the ED from her opioid use disorder clinic, where she was found to have an oxygen saturation by pulse oximetry (Spo2) of 82% on room air. S ... Full text Link to item Cite

Comparison of Creatinine, Urea, Ionized Calcium, and Bicarbonate Methods by 2 POC Systems and a Mainframe Chemistry Analyzer.

Journal Article J Appl Lab Med · March 6, 2023 BACKGROUND: The i-STAT (iST) and GEM Premier ChemSTAT (ChmST) are point-of-care systems that measure electrolytes, metabolites, acid-base parameters, and hematocrit on blood. We compared results for creatinine, urea (blood urea nitrogen [BUN]), total carbo ... Full text Link to item Cite

Evaluating the Association Between Fibrinogen and Rotational Thromboelastometry and the Progression to Severe Obstetric Hemorrhage.

Journal Article Clin Appl Thromb Hemost · 2023 AIM: This study aims to investigate the ability of fibrinogen and rotational thromboelastometry (ROTEM) parameters measured at obstetric hemorrhage protocol initiation to predict severe hemorrhage. METHODS: In this retrospective study we included patients ... Full text Link to item Cite
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Recent Grants


GEM® Premier¿ 5000 Critical Care System Pleural Fluid pH External Method Comparison Protoco

ResearchPrincipal Investigator · Awarded by Instrumentation Laboratory Company · 2018 - 2019

EDAN i15 Compartive Method Study

ResearchPrincipal Investigator · Awarded by EDAN Diagnostics, Inc · 2016 - 2017

GLUCOSE Monitor Meter with the GLUCOSE Monitor Test Strip High Altitude

ResearchPrincipal Investigator · Awarded by Roche Laboratories, Inc. · 2014 - 2015

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Education, Training & Certifications


University of North Carolina, Chapel Hill · 1977 Ph.D.
University of Florida · 1972 B.S.