Kathryn Meyer

Journal Article Nat Biotechnol · September 2024 The N6-methyladenosine (m6A) modification is found in thousands of cellular mRNAs and is a critical regulator of gene expression and cellular physiology. m6A dysregulation contributes to several human diseases, and the m6A methyltransferase machinery has e ... Full text Link to item Cite

mRNA epitranscriptomics.

Journal Article RNA · April 16, 2024 Epitranscriptomics refers to chemical changes in RNAs and includes numerous chemical types with varying stoichiometry and functions. RNA modifications are highly diverse in chemistry and respond in cell-type- and cell-state-dependent manners that enable an ... Full text Link to item Cite

Simultaneous In Situ Detection of m6A-Modified and Unmodified RNAs Using DART-FISH.

Chapter · 2024 N6-methyladenosine (m6A) is an abundant mRNA modification which plays important roles in regulating RNA function and gene expression. Traditional methods for visualizing mRNAs within cells cannot distinguish m6A-modified and unmodified versions of the targ ... Full text Link to item Cite

Studying m6A in the brain: a perspective on current methods, challenges, and future directions.

Journal Article Front Mol Neurosci · 2024 A major mechanism of post-transcriptional RNA regulation in cells is the addition of chemical modifications to RNA nucleosides, which contributes to nearly every aspect of the RNA life cycle. N6-methyladenosine (m6A) is a highly prevalent modification in c ... Full text Link to item Cite

Simultaneous profiling of the RNA targets of two RNA-binding proteins using TRIBE-STAMP.

Chapter · 2024 RNA-binding proteins (RBPs) are central players in RNA homeostasis and the control of gene expression. The identification of RBP targets, interactions, and the regulatory networks they control is crucial for understanding their cellular functions. Traditio ... Full text Link to item Cite

In situ visualization of m6A sites in cellular mRNAs.

Journal Article Nucleic Acids Res · November 10, 2023 N 6-methyladenosine (m6A) is an abundant RNA modification which plays critical roles in RNA function and cellular physiology. However, our understanding of how m6A is spatially regulated remains limited due to a lack of methods for visualizing methylated t ... Full text Link to item Cite

The Drosophila Nab2 RNA binding protein inhibits m6A methylation and male-specific splicing of Sex lethal transcript in female neuronal tissue.

Journal Article Elife · July 17, 2023 The Drosophila polyadenosine RNA binding protein Nab2, which is orthologous to a human protein lost in a form of inherited intellectual disability, controls adult locomotion, axon projection, dendritic arborization, and memory through a largely undefined s ... Full text Link to item Cite

The Proteins of mRNA Modification: Writers, Readers, and Erasers.

Journal Article Annu Rev Biochem · June 20, 2023 Over the past decade, mRNA modifications have emerged as important regulators of gene expression control in cells. Fueled in large part by the development of tools for detecting RNA modifications transcriptome wide, researchers have uncovered a diverse epi ... Full text Link to item Cite

Abstract 2497: New tools for detecting mRNA methylation

Conference Journal of Biological Chemistry · 2023 Full text Cite

Exploring the brain epitranscriptome: perspectives from the NSAS summit.

Journal Article Front Neurosci · 2023 Increasing evidence reinforces the essential function of RNA modifications in development and diseases, especially in the nervous system. RNA modifications impact various processes in the brain, including neurodevelopment, neurogenesis, neuroplasticity, le ... Full text Open Access Link to item Cite

Epitranscriptomics in parasitic protists: Role of RNA chemical modifications in posttranscriptional gene regulation.

Journal Article PLoS Pathog · December 2022 "Epitranscriptomics" is the new RNA code that represents an ensemble of posttranscriptional RNA chemical modifications, which can precisely coordinate gene expression and biological processes. There are several RNA base modifications, such as N6-methyladen ... Full text Open Access Link to item Cite

Detection of m6A in single cultured cells using scDART-seq.

Journal Article STAR Protoc · September 16, 2022 Most techniques for mapping m6A-methylated RNAs transcriptome-wide require large amounts of RNA and have been limited to bulk cells and tissues. Here, we provide a detailed protocol for the identification of m6A sites in single HEK293T cells using single-c ... Full text Link to item Cite

Single-molecule identification of the target RNAs of different RNA binding proteins simultaneously in cells.

Journal Article Genes & development · September 2022 RNA-binding proteins (RBPs) regulate nearly every aspect of mRNA processing and are important regulators of gene expression in cells. However, current methods for transcriptome-wide identification of RBP targets are limited, since they examine only a singl ... Full text Cite

RBM45 is an m6A-binding protein that affects neuronal differentiation and the splicing of a subset of mRNAs.

Journal Article Cell Rep · August 30, 2022 N6-methyladenosine (m6A) is deposited co-transcriptionally on thousands of cellular mRNAs and plays important roles in mRNA processing and cellular function. m6A is particularly abundant within the brain and is critical for neurodevelopment. However, the m ... Full text Link to item Cite

How m6A makes its mark.

Journal Article Nat Rev Mol Cell Biol · August 2022 Full text Link to item Cite

m6A and YTHDF proteins contribute to the localization of select neuronal mRNAs.

Journal Article Nucleic Acids Res · May 6, 2022 The transport of mRNAs to distal subcellular compartments is an important component of spatial gene expression control in neurons. However, the mechanisms that control mRNA localization in neurons are not completely understood. Here, we identify the abunda ... Full text Link to item Cite

scDART-seq reveals distinct m6A signatures and mRNA methylation heterogeneity in single cells.

Journal Article Mol Cell · February 17, 2022 N6-methyladenosine (m6A) is an abundant RNA modification that plays critical roles in RNA regulation and cellular function. Global m6A profiling has revealed important aspects of m6A distribution and function, but to date such studies have been restricted ... Full text Link to item Cite

Detecting m6A with In Vitro DART-Seq.

Chapter · 2022 Recent studies have uncovered that cellular mRNAs contain a diverse epitranscriptome comprising chemically modified bases which play important roles in gene expression regulation. Among these is m6A, which is a highly prevalent modification that contribute ... Full text Link to item Cite

Improved Methods for Deamination-Based m6A Detection.

Journal Article Front Cell Dev Biol · 2022 N 6-methyladenosine (m6A) is a critical regulator of gene expression and cellular function. Much of our knowledge of m6A has been enabled by the identification of m6A sites transcriptome-wide. However, global m6A profiling methods require high amounts of i ... Full text Link to item Cite

Mapping of m6A and Its Regulatory Targets in Prostate Cancer Reveals a METTL3-Low Induction of Therapy Resistance.

Journal Article Mol Cancer Res · August 2021 Recent evidence has highlighted the role of N 6-methyladenosine (m6A) in the regulation of mRNA expression, stability, and translation, supporting a potential role for posttranscriptional regulation mediated by m6A in cancer. Here, we explore prostate canc ... Full text Link to item Cite

RNA N6-Methyladenosine and the Regulation of RNA Localization and Function in the Brain.

Journal Article Trends Neurosci · December 2020 A major challenge in neurobiology in the 21st century is to understand how the brain adapts with experience. Activity-dependent gene expression is integral to the synaptic plasticity underlying learning and memory; however, this process cannot be explained ... Full text Link to item Cite

The epitranscriptome and synaptic plasticity.

Journal Article Curr Opin Neurobiol · December 2019 RNA modifications, collectively referred to as 'the epitranscriptome,' have recently emerged as a pervasive feature of cellular mRNAs which have diverse impacts on gene expression. In the last several years, technological advances improving our ability to ... Full text Link to item Cite

DART-seq: an antibody-free method for global m6A detection.

Journal Article Nat Methods · December 2019 N6-methyladenosine (m6A) is a widespread RNA modification that influences nearly every aspect of the messenger RNA lifecycle. Our understanding of m6A has been facilitated by the development of global m6A mapping methods, which use antibodies to immunoprec ... Full text Link to item Cite

Transcriptome-wide m6A detection with DART-Seq

Journal Article · September 23, 2019 Abstractm6A is the most abundant internal mRNA modification and plays diverse roles in gene expression regulation. Much of our current knowledge about m6A has been driven b ... Full text Cite

m6A-mediated translation regulation.

Journal Article Biochim Biophys Acta Gene Regul Mech · March 2019 Full text Link to item Cite

Acetylation takes aim at mRNA.

Journal Article Nat Struct Mol Biol · December 2018 Full text Link to item Cite

A potentially abundant junctional RNA motif stabilized by m6A and Mg2.

Journal Article Nat Commun · July 17, 2018 N6-Methyladenosine (m6A) is an abundant post-transcriptional RNA modification that influences multiple aspects of gene expression. In addition to recruiting proteins, m6A can modulate RNA function by destabilizing base pairing. Here, we show that when neig ... Full text Link to item Cite

Rethinking m6A Readers, Writers, and Erasers.

Journal Article Annu Rev Cell Dev Biol · October 6, 2017 In recent years, m6A has emerged as an abundant and dynamically regulated modification throughout the transcriptome. Recent technological advances have enabled the transcriptome-wide identification of m6A residues, which in turn has provided important insi ... Full text Link to item Cite

Covalent Modifications to mRNA, or the "Epitranscriptome".

Conference ENVIRONMENTAL AND MOLECULAR MUTAGENESIS · September 1, 2017 Link to item Cite

Expanding the diversity of DNA base modifications with N⁶-methyldeoxyadenosine.

Journal Article Genome Biol · January 13, 2016 Vertebrate DNA is subjected to epigenetic base modifications that have been thought to be limited to methylated and other modified forms of cytidine. A recent study shows that methylation of adenine to form N (6)-methyladenine is a rare but readily detecta ... Full text Link to item Cite

5' UTR m(6)A Promotes Cap-Independent Translation.

Journal Article Cell · November 5, 2015 Protein translation typically begins with the recruitment of the 43S ribosomal complex to the 5' cap of mRNAs by a cap-binding complex. However, some transcripts are translated in a cap-independent manner through poorly understood mechanisms. Here, we show ... Full text Link to item Cite

Novel RNA modifications in the nervous system: form and function.

Journal Article J Neurosci · November 12, 2014 Modified RNA molecules have recently been shown to regulate nervous system functions. This mini-review and associated mini-symposium provide an overview of the types and known functions of novel modified RNAs in the nervous system, including covalently mod ... Full text Link to item Cite

The dynamic epitranscriptome: N6-methyladenosine and gene expression control.

Journal Article Nat Rev Mol Cell Biol · May 2014 N(6)-methyladenosine (m(6)A) is a modified base that has long been known to be present in non-coding RNAs, ribosomal RNA, polyadenylated RNA and at least one mammalian mRNA. However, our understanding of the prevalence of this modification has been fundame ... Full text Link to item Cite

The fat mass and obesity associated gene (Fto) regulates activity of the dopaminergic midbrain circuitry.

Journal Article Nat Neurosci · August 2013 Dopaminergic (DA) signaling governs the control of complex behaviors, and its deregulation has been implicated in a wide range of diseases. Here we demonstrate that inactivation of the Fto gene, encoding a nucleic acid demethylase, impairs dopamine recepto ... Full text Link to item Cite

The birth of the Epitranscriptome: deciphering the function of RNA modifications.

Journal Article Genome Biol · October 31, 2012 Recent studies have found methyl-6-adenosine in thousands of mammalian genes, and this modification is most pronounced near the beginning of the 3' UTR. We present a perspective on current work and new single-molecule sequencing methods for detecting RNA b ... Full text Link to item Cite

Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons.

Journal Article Cell · June 22, 2012 Methylation of the N(6) position of adenosine (m(6)A) is a posttranscriptional modification of RNA with poorly understood prevalence and physiological relevance. The recent discovery that FTO, an obesity risk gene, encodes an m(6)A demethylase implicates m ... Full text Link to item Cite

Disc1 regulates granule cell migration in the developing hippocampus.

Journal Article Hum Mol Genet · September 1, 2009 Schizophrenia is a severely debilitating psychiatric disease that is hypothesized to have its roots in neurodevelopment. Although the precise neuropathology underlying schizophrenia has remained elusive, there are consistent reports of abnormalities in sev ... Full text Link to item Cite

Immunohistochemical analysis of Disc1 expression in the developing and adult hippocampus.

Journal Article Gene Expr Patterns · September 2008 In recent years, Disrupted-In-Schizophrenia 1 (DISC1) has emerged as one of the most promising candidate genes whose disruption confers an increased risk for schizophrenia. Cell biology studies have implicated DISC1 in key neurodevelopmental processes incl ... Full text Link to item Cite