Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • Postdoctoral Associate, Chemistry, Princeton University 2010 - 2015
  • Ph.D., Stanford University 2010
  • B.Sc., Emory University 2005
• 

  Previous Appointments & Affiliations

  • Assistant Professor of Chemistry, Chemistry, Trinity College of Arts & Sciences 2015 - 2022
• Recognition
• 

  In the News

  • NOV 10, 2022

    Watch a Virus in the Moments Right Before it Attacks

  • JUN 22, 2017

    Duke Research Blog

    3D Virus Cam Catches Germs Red-Handed

  • OCT 1, 2015

    Kevin Welsher: Blocking the Virus Torpedo
• Expertise
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  Subject Headings

  • Muser Mentor
• Research
• 

  Selected Grants

  • Revealing Hidden Behaviors during Polymerization via Single-Molecular-Catalyst and Single Polymer-Particle Microscopy awarded by University of California - Irvine 2022 - 2025
  • CAREER: Target-locked single molecule spectroscopy and super-resolution microscopy awarded by  National Science Foundation 2019 - 2024
  • LUMICKS C-TRAP awarded by National Institutes of Health 2022 - 2023
  • Unraveling the Viral Infection Pathway with Virus-locked Observation awarded by National Institutes of Health 2017 - 2022
• Publications & Artistic Works
• 

  Selected Publications

  • Academic Articles

    • Johnson, Courtney, Jack Exell, Yuxin Lin, Jonathan Aguilar, and Kevin D. Welsher. “Capturing the start point of the virus-cell interaction with high-speed 3D single-virus tracking.” Nature Methods 19, no. 12 (December 2022): 1642–52. https://doi.org/10.1038/s41592-022-01672-3.
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    • Niver, Anastasia J., and Kevin D. Welsher. “Combined online Bayesian and windowed estimation of background and signal localization facilitates active-feedback particle tracking in complex environments.” The Journal of Chemical Physics 157, no. 18 (November 2022): 184108. https://doi.org/10.1063/5.0118317.
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    • Kim, Jeonghwan, Antony Jozic, Yuxin Lin, Yulia Eygeris, Elissa Bloom, Xiaochen Tan, Christopher Acosta, Kelvin D. MacDonald, Kevin D. Welsher, and Gaurav Sahay. “Engineering Lipid Nanoparticles for Enhanced Intracellular Delivery of mRNA through Inhalation.” Acs Nano 16, no. 9 (September 2022): 14792–806. https://doi.org/10.1021/acsnano.2c05647.
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    • Yu, Donggeng, Antonio Garcia, Suzanne A. Blum, and Kevin D. Welsher. “Growth Kinetics of Single Polymer Particles in Solution via Active-Feedback 3D Tracking.” Journal of the American Chemical Society 144, no. 32 (August 2022): 14698–705. https://doi.org/10.1021/jacs.2c04990.
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    • Li, W. Y., S. Yin, S. W. Huang, M. H. Yang, P. M. Chen, S. R. Wu, K. Welsher, H. Yang, and Y. M. Arthur Chen. “The trajectory patterns of single HIV-1 virus-like particle in live CD4 cells: A real time three-dimensional multi-resolution microscopy study using encapsulated nonblinking giant quantum dot.” Journal of Microbiology, Immunology and Infection, January 1, 2022. https://doi.org/10.1016/j.jmii.2022.08.011.
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    • Tan, Xiaochen, and Kevin Welsher. “Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*.” Angewandte Chemie (International Ed. in English) 60, no. 41 (October 2021): 22359–67. https://doi.org/10.1002/anie.202105741.
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    • Zhang, Chen, and Kevin Welsher. “Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy.” Entropy (Basel, Switzerland) 23, no. 5 (April 2021): 498. https://doi.org/10.3390/e23050498.
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    • Hou, Shangguo, Jack Exell, and Kevin Welsher. “Real-time 3D single molecule tracking.” Nature Communications 11, no. 1 (July 2020): 3607. https://doi.org/10.1038/s41467-020-17444-6.
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    • Patel, Siddharth, N. Ashwanikumar, Ema Robinson, Yan Xia, Cosmin Mihai, Joseph P. Griffith, Shangguo Hou, et al. “Author Correction: Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA.” Nature Communications 11, no. 1 (July 2020): 3435. https://doi.org/10.1038/s41467-020-17025-7.
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    • Lang, Xiaoqi, and Kevin Welsher. “Mapping solvation heterogeneity in live cells by hyperspectral stimulated Raman scattering microscopy.” The Journal of Chemical Physics 152, no. 17 (May 2020): 174201. https://doi.org/10.1063/1.5141422.
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    • Patel, Siddharth, N. Ashwanikumar, Ema Robinson, Yan Xia, Cosmin Mihai, Joseph P. Griffith, Shangguo Hou, et al. “Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA.” Nature Communications 11, no. 1 (February 2020): 983. https://doi.org/10.1038/s41467-020-14527-2.
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    • Johnson, Courtney, Jack Exell, Jonathon Kuo, and Kevin Welsher. “Continuous focal translation enhances rate of point-scan volumetric microscopy.” Optics Express 27, no. 25 (December 2019): 36241–58. https://doi.org/10.1364/oe.27.036241.
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    • Hou, Shangguo, and Kevin Welsher. “An Adaptive Real-Time 3D Single Particle Tracking Method for Monitoring Viral First Contacts.” Small (Weinheim an Der Bergstrasse, Germany) 15, no. 44 (October 2019): e1903039. https://doi.org/10.1002/smll.201903039.
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    • Hou, Shangguo, Courtney Johnson, and Kevin Welsher. “Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells.” Molecules (Basel, Switzerland) 24, no. 15 (August 2019): E2826. https://doi.org/10.3390/molecules24152826.
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    • Hou, Shangguo, and Kevin Welsher. “A Protocol for Real-time 3D Single Particle Tracking.” Journal of Visualized Experiments : Jove, no. 131 (January 2018). https://doi.org/10.3791/56711.
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    • Hou, Shangguo, Xiaoqi Lang, and Kevin Welsher. “Robust real-time 3D single-particle tracking using a dynamically moving laser spot.” Optics Letters 42, no. 12 (June 2017): 2390–93. https://doi.org/10.1364/ol.42.002390.
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    • Welsher, Kevin, and Haw Yang. “Imaging the behavior of molecules in biological systems: breaking the 3D speed barrier with 3D multi-resolution microscopy.” Faraday Discussions 184 (January 2015): 359–79. https://doi.org/10.1039/c5fd00090d.
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    • Welsher, Kevin, Simon A. McManus, Chih-Hao Hsia, Shuhui Yin, and Haw Yang. “Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning.” Journal of the American Chemical Society 137, no. 2 (January 2015): 580–83. https://doi.org/10.1021/ja511297d.
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    • Welsher, Kevin, and Haw Yang. “Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles.” Nature Nanotechnology 9, no. 3 (March 2014): 198–203. https://doi.org/10.1038/nnano.2014.12.
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    • Welsher, K., and H. Yang. “Three-dimensional target-locking microscopy.” Optics Infobase Conference Papers, January 1, 2013.
    • Welsher, K., and H. Yang. “Model-free analysis of time-dependent single-molecule spectroscopy: Dynamics of biological macromolecules.” Proceedings  International Symposium on Biomedical Imaging, August 15, 2012, 921–23. https://doi.org/10.1109/ISBI.2012.6235705.
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    • Chen, Zhuo, Guosong Hong, Hailiang Wang, Kevin Welsher, Scott M. Tabakman, Sarah P. Sherlock, Joshua T. Robinson, Yongye Liang, and Hongjie Dai. “Graphite-coated magnetic nanoparticle microarray for few-cells enrichment and detection.” Acs Nano 6, no. 2 (February 2012): 1094–1101. https://doi.org/10.1021/nn2034692.
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    • Hong, Guosong, Scott M. Tabakman, Kevin Welsher, Zhuo Chen, Joshua T. Robinson, Hailiang Wang, Bo Zhang, and Hongjie Dai. “Near-infrared-fluorescence-enhanced molecular imaging of live cells on gold substrates.” Angewandte Chemie (International Ed. in English) 50, no. 20 (May 2011): 4644–48. https://doi.org/10.1002/anie.201100934.
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    • Welsher, Kevin, Sarah P. Sherlock, and Hongjie Dai. “Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared window.” Proceedings of the National Academy of Sciences of the United States of America 108, no. 22 (May 2011): 8943–48. https://doi.org/10.1073/pnas.1014501108.
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    • Tabakman, S. M., K. Welsher, G. Hong, and H. Dai. “Optical properties of single-walled carbon nanotubes separated in a density gradient: Length, bundling, and aromatic stacking effects.” Journal of Physical Chemistry C 114, no. 46 (November 25, 2010): 19569–75. https://doi.org/10.1021/jp106453v.
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    • Hong, Guosong, Scott M. Tabakman, Kevin Welsher, Hailiang Wang, Xinran Wang, and Hongjie Dai. “Metal-enhanced fluorescence of carbon nanotubes.” Journal of the American Chemical Society 132, no. 45 (November 2010): 15920–23. https://doi.org/10.1021/ja1087997.
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    • Robinson, J. T., K. Welsher, S. M. Tabakman, S. P. Sherlock, H. Wang, R. Luong, and H. Dai. “High performance in vivo near-IR (>1 μm) imaging and photothermal cancer therapy with carbon nanotubes.” Nano Research 3, no. 11 (January 1, 2010): 779–93. https://doi.org/10.1007/s12274-010-0045-1.
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    • Welsher, Kevin, Zhuang Liu, Sarah P. Sherlock, Joshua Tucker Robinson, Zhuo Chen, Dan Daranciang, and Hongjie Dai. “A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice.” Nature Nanotechnology 4, no. 11 (November 2009): 773–80. https://doi.org/10.1038/nnano.2009.294.
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    • Prencipe, Giuseppe, Scott M. Tabakman, Kevin Welsher, Zhuang Liu, Andrew P. Goodwin, Li Zhang, Joy Henry, and Hongjie Dai. “PEG branched polymer for functionalization of nanomaterials with ultralong blood circulation.” Journal of the American Chemical Society 131, no. 13 (April 2009): 4783–87. https://doi.org/10.1021/ja809086q.
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    • Zhang, Li, Xiaomin Tu, Kevin Welsher, Xinran Wang, Ming Zheng, and Hongjie Dai. “Optical characterizations and electronic devices of nearly pure (10,5) single-walled carbon nanotubes.” Journal of the American Chemical Society 131, no. 7 (February 2009): 2454–55. https://doi.org/10.1021/ja8096674.
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    • Goodwin, Andrew P., Scott M. Tabakman, Kevin Welsher, Sarah P. Sherlock, Giuseppe Prencipe, and Hongjie Dai. “Phospholipid-dextran with a single coupling point: a useful amphiphile for functionalization of nanomaterials.” Journal of the American Chemical Society 131, no. 1 (January 2009): 289–96. https://doi.org/10.1021/ja807307e.
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    • Liu, Z., S. Tabakman, K. Welsher, and H. Dai. “Carbon nanotubes in biology and medicine: In vitro and in vivo detection, imaging and drug delivery.” Nano Research 2, no. 2 (January 1, 2009): 85–120. https://doi.org/10.1007/s12274-009-9009-8.
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    • Sun, X., S. Zaric, D. Daranciang, K. Welsher, Y. Lu, X. Li, and H. Dai. “Optical properties of ultrashort semiconducting single-walled carbon nanotube capsules down to sub-10 nm.” Journal of the American Chemical Society 130, no. 20 (May 21, 2008): 6551–55. https://doi.org/10.1021/ja8006929.
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    • Welsher, Kevin, Zhuang Liu, Dan Daranciang, and Hongjie Dai. “Selective probing and imaging of cells with single walled carbon nanotubes as near-infrared fluorescent molecules.” Nano Letters 8, no. 2 (February 2008): 586–90. https://doi.org/10.1021/nl072949q.
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    • Li, X., X. Tu, S. Zaric, K. Welsher, S. S. Won, W. Zhao, and H. Dai. “Selective synthesis combined with chemical separation of single-walled carbon nanotubes for chirality selection.” Journal of the American Chemical Society 129, no. 51 (December 26, 2007): 15770–71. https://doi.org/10.1021/ja077886s.
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    • Nakayama-Ratchford, Nozomi, Sarunya Bangsaruntip, Xiaoming Sun, Kevin Welsher, and Hongjie Dai. “Noncovalent functionalization of carbon nanotubes by fluorescein-polyethylene glycol: supramolecular conjugates with pH-dependent absorbance and fluorescence.” Journal of the American Chemical Society 129, no. 9 (March 2007): 2448–49. https://doi.org/10.1021/ja068684j.
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  • Conference Papers

    • Johnson, C., and K. Welsher. “Point-scan volumetric imaging rate increased by an optimized linear sparse sampling pattern.” In Frontiers in Optics  Proceedings Frontiers in Optics + Laser Science Aps/Dls, 2019. https://doi.org/10.1364/FIO.2019.JTu4A.101.
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    • Welsher, K., and H. Yang. “Three-dimensional target-locking microscopy.” In Optics Infobase Conference Papers, 2013. https://doi.org/10.1364/ls.2013.lth3h.2.
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  • Datasets

    • Welsher, Kevin, Shangguo Hou, and Anastasia Niver. “Data from: Mapping nanoscale forces and potentials in live cells with microsecond 3D single-particle tracking,” December 9, 2022. https://doi.org/10.7924/r4dv1q87f.
       Data Access
• Teaching & Mentoring
• 

  Recent Courses

  • CHEM 393: Research Independent Study 2023
  • CHEM 394: Research Independent Study 2023
  • CHEM 493: Research Independent Study 2023
  • CHEM 494: Research Independent Study 2023
  • CHEM 590: Special Topics in Chemistry 2023
  • CHEM 301: Elements of Physical Chemistry 2022
  • CHEM 393: Research Independent Study 2022
  • CHEM 394: Research Independent Study 2022
  • CHEM 493: Research Independent Study 2022
  • CHEM 494: Research Independent Study 2022
  • CHEM 590: Special Topics in Chemistry 2022
  • CHEM 301: Elements of Physical Chemistry 2021
  • CHEM 393: Research Independent Study 2021
  • CHEM 394: Research Independent Study 2021
  • CHEM 493: Research Independent Study 2021
  • CHEM 494: Research Independent Study 2021
  • CHEM 590: Special Topics in Chemistry 2021

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