Linfeng Huang | Scholars@Duke

Linfeng Huang
Associate Professor of Biology at Duke Kunshan University

Prof. Linfeng Huang received his Bachelor of Science degree from the College of Biological Sciences, China Agricultural University in Beijing in 2003. After graduation, he did a short academic visit at the Centre for Ecology and Hydrology - Oxford and then studied his PhD with Prof. Sir David Baulcombe at the Sainsbury Laboratory, the John Innes Centre in the UK. He obtained his PhD degree from the University of East Anglia in 2009. Between 2009 and 2014, he did his postdoctoral training with Prof. Judy Lieberman at Boston Children’s Hospital and Harvard Medical School in the USA. Since Aug 2014, he was an Assistant Professor and then an Associate Professor (tenured) at the Department of Biomedical Sciences, City University of Hong Kong. Prof. Huang joined Duke Kunshan University as an Associate Professor of Biology in Sep 2020.

Current Research Interests

Prof. Huang is passionate about pursuing fundamental questions in RNA biology and firmly believes such knowledge can be exploited to create powerful RNA-based biotechnology and therapeutics. His research is mainly in the RNA interference (RNAi) field. Recently he discovered a species of bacterial small RNAs which is similar to eukaryotic small interfering RNA (siRNA). Those bacterial small RNAs, named pro-siRNAs (for prokaryotic siRNAs), were stabilized by ectopic expression of p19, a plant viral siRNA binding protein. Based on this discovery, he invented a technology of producing gene specific and highly efficient ‘recombinant’ siRNAs in bacteria.
Currently, Prof. Huang’s research focuses on developing pro-siRNA-based RNAi technology and using high-throughput RNAi screen to study human diseases.

Current Appointments & Affiliations

Associate Professor of Biology at Duke Kunshan University, DKU Faculty, Duke Kunshan University 2020

Contact Information

Background
Education, Training, & Certifications
- Ph.D., University of East Anglia (United Kingdom) 2009
Expertise
Subject Headings
Publications & Artistic Works
Selected Publications
- Academic Articles
  - Xie, Kai, Zixun Wang, Lin Qi, Xi Zhao, Yuan Wang, Jin Qu, Ping Xu, et al. “Profiling MicroRNAs with Associated Spatial Dynamics in Acute Tissue Slices.” Acs Nano 15, no. 3 (March 10, 2021): 4881–92. https://doi.org/10.1021/acsnano.0c09676.
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  - Shi, Shanwei, Huigen Luo, Lihong Wang, Hua Li, Yujie Liang, Juan Xia, Zhi Wang, et al. “Combined inhibition of RNA polymerase I and mTORC1/2 synergize to combat oral squamous cell carcinoma.” Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie 133 (January 2021): 110906. https://doi.org/10.1016/j.biopha.2020.110906.
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  - Wang, Yuan, Zixun Wang, Kai Xie, Xi Zhao, Xuezhen Jiang, Bing Chen, Ying Han, et al. “High-Efficiency Cellular Reprogramming by Nanoscale Puncturing.” Nano Letters 20, no. 7 (July 2020): 5473–81. https://doi.org/10.1021/acs.nanolett.0c01979.
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  - Wang, Zixun, Lin Qi, Yang Yang, Mingxing Lu, Kai Xie, Xi Zhao, Elvis Hung Chi Cheung, et al. “High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity.” Science Advances 6, no. 24 (June 10, 2020): eaba4971. https://doi.org/10.1126/sciadv.aba4971.
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  - Huang, Linfeng, Padraig Deighan, Jingmin Jin, Yingxue Li, Hung-Chi Cheung, Elaine Lee, Shirley S. Mo, et al. “Tombusvirus p19 Captures RNase III-Cleaved Double-Stranded RNAs Formed by Overlapping Sense and Antisense Transcripts in Escherichia coli.” Mbio 11, no. 3 (June 9, 2020). https://doi.org/10.1128/mbio.00485-20.
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  - Li, Tianzhong, Linfeng Huang, and Mengsu Yang. “Lipid-based Vehicles for siRNA Delivery in Biomedical Field.” Current Pharmaceutical Biotechnology 21, no. 1 (January 2020): 3–22. https://doi.org/10.2174/1389201020666190924164152.
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  - Li, Cai, Tianzhong Li, Linfeng Huang, Mengsu Yang, and Guangyu Zhu. “Self-assembled Lipid Nanoparticles for Ratiometric Codelivery of Cisplatin and siRNA Targeting XPF to Combat Drug Resistance in Lung Cancer.” Chemistry, an Asian Journal 14, no. 9 (May 2019): 1570–76. https://doi.org/10.1002/asia.201900005.
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  - Li, Tianzhong, Jianbo Yue, Linfeng Huang, and Mengsu Yang. “Autophagy inhibitor Vacuolin-1 interferes with lipid-based small interference RNA delivery.” Biochemical and Biophysical Research Communications 510, no. 3 (March 2019): 427–34. https://doi.org/10.1016/j.bbrc.2019.01.121.
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  - Vu, Luyen Tien, Boya Peng, Daniel Xin Zhang, Victor Ma, Camille A. Mathey-Andrews, Chun Kuen Lam, Theodoros Kiomourtzis, et al. “Tumor-secreted extracellular vesicles promote the activation of cancer-associated fibroblasts via the transfer of microRNA-125b.” Journal of Extracellular Vesicles 8, no. 1 (January 2019): 1599680. https://doi.org/10.1080/20013078.2019.1599680.
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  - Kaur, Guneet, Hung-Chi Cheung, Wei Xu, Jun Vic Wong, For Fan Chan, Yingxue Li, Larry McReynolds, and Linfeng Huang. “Milligram scale production of potent recombinant small interfering RNAs in Escherichia coli.” Biotechnology and Bioengineering 115, no. 9 (September 2018): 2280–91. https://doi.org/10.1002/bit.26740.
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  - Xu, Zhen, Wei Wang, Yutian Ren, Wenchong Zhang, Peilin Fang, Linfeng Huang, Xin Wang, and Peng Shi. “Regeneration of cortical tissue from brain injury by implantation of defined molecular gradient of semaphorin 3A.” Biomaterials 157 (March 2018): 125–35. https://doi.org/10.1016/j.biomaterials.2017.12.010.
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  - Huang, Linfeng, and Judy Lieberman. “Production of highly potent recombinant siRNAs in Escherichia coli.” Nature Protocols 8, no. 12 (December 2013): 2325–36. https://doi.org/10.1038/nprot.2013.149.
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  - Huang, Linfeng, Jingmin Jin, Padraig Deighan, Evgeny Kiner, Larry McReynolds, and Judy Lieberman. “Efficient and specific gene knockdown by small interfering RNAs produced in bacteria.” Nature Biotechnology 31, no. 4 (April 2013): 350–56. https://doi.org/10.1038/nbt.2537.
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  - Ho, Thien, Liang Wang, Linfeng Huang, Zhigang Li, Denise W. Pallett, Tamas Dalmay, Kazusato Ohshima, John A. Walsh, and Hui Wang. “Nucleotide bias of DCL and AGO in plant anti-virus gene silencing.” Protein & Cell 1, no. 9 (September 2010): 847–58. https://doi.org/10.1007/s13238-010-0100-4.
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  - Huang, Linfeng, Alexandra M. E. Jones, Iain Searle, Kanu Patel, Hannes Vogler, Nina C. Hubner, and David C. Baulcombe. “An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II.” Nature Structural & Molecular Biology 16, no. 1 (January 2009): 91–93. https://doi.org/10.1038/nsmb.1539.
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  - Wang, H., L. F. Huang, and J. I. Cooper. “Analyses on mutation patterns, detection of population bottlenecks, and suggestion of deleterious-compensatory evolution among members of the genus Potyvirus.” Archives of Virology 151, no. 8 (August 2006): 1625–33. https://doi.org/10.1007/s00705-006-0741-z.
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  - Huang, Wei E., Linfeng Huang, Gail M. Preston, Martin Naylor, John P. Carr, Yanhong Li, Andrew C. Singer, Andrew S. Whiteley, and Hui Wang. “Quantitative in situ assay of salicylic acid in tobacco leaves using a genetically modified biosensor strain of Acinetobacter sp. ADP1.” The Plant Journal : For Cell and Molecular Biology 46, no. 6 (June 2006): 1073–83. https://doi.org/10.1111/j.1365-313x.2006.02758.x.
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  - Pallett, D. W., L. Huang, J. I. Cooper, and H. Wang. “Within-population variation in hybridisation and transgene transfer between wild Brassica rapa and Brassica napus in the UK.” Annals of Applied Biology 148, no. 2 (April 1, 2006): 147–55. https://doi.org/10.1111/j.1744-7348.2006.00049.x.
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  - Huang, L. F., M. Naylor, D. W. Pallett, J. Reeves, J. I. Cooper, and H. Wang. “The complete genome sequence, organization and affinities of carrot red leaf virus.” Archives of Virology 150, no. 9 (September 2005): 1845–55. https://doi.org/10.1007/s00705-005-0537-6.
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  - Huang, Wei E., Hui Wang, Hongjun Zheng, Linfeng Huang, Andrew C. Singer, Ian Thompson, and Andrew S. Whiteley. “Chromosomally located gene fusions constructed in Acinetobacter sp. ADP1 for the detection of salicylate.” Environmental Microbiology 7, no. 9 (September 2005): 1339–48. https://doi.org/10.1111/j.1462-5822.2005.00821.x.
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  - Zhou, H., H. Wang, L. F. Huang, M. Naylor, and P. Clifford. “Heterogeneity in codon usages of sobemovirus genes.” Archives of Virology 150, no. 8 (August 2005): 1591–1605. https://doi.org/10.1007/s00705-005-0510-4.
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- Book Sections
  - Xu, W., X. Jiang, and L. Huang. “RNA interference technology.” In Comprehensive Biotechnology, 560–75, 2019. https://doi.org/10.1016/B978-0-444-64046-8.00282-2.
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Teaching & Mentoring
Teaching Activities
- Course at Duke Kunshan University
  
  BIOL 212 Microbiology
  
  BIOL 304 Molecular Genetic Analysis
  
  BIOL 201 Cell and Molecular Biology

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Education, Training, & Certifications

Subject Headings

Selected Publications

Academic Articles

Book Sections

Teaching Activities