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Lisa L Satterwhite

Lisa L Satterwhite

Assistant Research Professor in the Department of Civil and Environmental Engineering
Civil and Environmental Engineering
lisa.satterwhite@duke.edu
Box 90287, Durham, NC 27708-0287
CIEMAS 2447B, Durham, NC 27708

Selected Publications

Epigenetic and transcriptional responses in circulating leukocytes are associated with future decompensation during SARS-CoV-2 infection.

Journal Article iScience · January 19, 2024 To elucidate host response elements that define impending decompensation during SARS-CoV-2 infection, we enrolled subjects hospitalized with COVID-19 who were matched for disease severity and comorbidities at the time of admission. We performed combined si ... Full text Open Access Link to item Cite

Differential chromatin accessibility in peripheral blood mononuclear cells underlies COVID-19 disease severity prior to seroconversion.

Journal Article Sci Rep · July 9, 2022 SARS-CoV-2 infection triggers profound and variable immune responses in human hosts. Chromatin remodeling has been observed in individuals severely ill or convalescing with COVID-19, but chromatin remodeling early in disease prior to anti-spike protein IgG ... Full text Open Access Link to item Cite

Quantitative phase microscopy spatial signatures of cancer cells.

Journal Article Cytometry. Part A : the journal of the International Society for Analytical Cytology · May 2017 We present cytometric classification of live healthy and cancerous cells by using the spatial morphological and textural information found in the label-free quantitative phase images of the cells. We compare both healthy cells to primary tumor cells and pr ... Full text Cite

Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers.

Journal Article Acta biomaterialia · February 2014 Patients with coronary artery disease (CAD) are the primary candidates to receive small-diameter tissue-engineered blood vessels (TEBVs). Peripheral blood derived endothelial progenitor cells (EPCs) from CAD patients (CAD EPCs) represent a minimally invasi ... Full text Cite

Biomedical Optical Phase Microscopy and Nanoscopy

Journal Article Biomedical Optical Phase Microscopy and Nanoscopy · August 26, 2013 Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, ... Cite

Biomedical Optical Phase Microscopy and Nanoscopy

Book · January 1, 2013 Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, ... Full text Cite

Preface

Journal Article Biomedical Optical Phase Microscopy and Nanoscopy · January 1, 2013 Full text Cite

Dynamic quantitative microscopy and nanoscopy of red blood cells in sickle cell disease

Conference Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 16, 2012 We have applied wide-field digital interferometric techniques to quantitatively image sickle red blood cells (RBCs) [1] in a noncontact label-free manner, and measure the nanometer-scale fluctuations in their thickness as an indication of their stiffness. ... Full text Cite

Spectral-domain differential interference contrast (SD-DIC) microscopy for measuring live cell dynamics

Journal Article Biomedical Optics, BIOMED 2012 · January 1, 2012 We present live cell imaging using spectral-domain differential interference contrast microscopy, a novel technique for high-resolution, quantitative measurement of optical pathlength gradients. Imaging and dynamic monitoring of live neurons and cardiomyoc ... Full text Cite

Novel optical signature for sickle cell trait red blood cells

Journal Article Frontiers in Optics, FIO 2012 · January 1, 2012 We identified unique optical signatures for sickle cell trait, a condition where heterozygous individuals are carriers for the hemoglobin allele that causes sickle cell anemia, by using wide-field interferometric microscopy. © OSA 2012. ... Full text Cite

Nonlinear phase dispersion spectroscopy.

Journal Article Optics letters · December 2011 Nonlinear phase dispersion spectroscopy is introduced as a means to retrieve wideband, high spectral resolution profiles of the wavelength-dependent real part of the refractive index. The method is based on detecting dispersion effects imparted to a light ... Full text Cite

Whole cell imaging based on wide-field interferometric phase microscopy and its application to cardiomyocytes

Journal Article Progress in Biomedical Optics and Imaging - Proceedings of SPIE · April 29, 2011 Whole cell imaging is a novel technique using which the time-dependent quantitative phase profiles of live unstained biological cells are analyzed numerically to learn on the cell functionally. Dynamic phase profiles of the sample are first acquired by wid ... Full text Cite

Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry.

Journal Article J Biomed Opt · March 2011 We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical propertie ... Full text Link to item Cite

Spectral-domain differential interference contrast microscopy.

Journal Article Optics letters · February 2011 We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the comm ... Full text Cite

Spectral-domain differential interference contrast microscopy

Journal Article Optics InfoBase Conference Papers · 2011 Cite

Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy

Journal Article Optics InfoBase Conference Papers · 2011 We have developed a novel microscope combining quantitative phase and fluorescence microscopy to perform quantitative analysis of dynamic cardiomyocyte contraction. Phase-based parameters are informed by molecular specificity of fluorescence images. © 2011 ... Cite

Real-time quantitative phase and dual-channel fluorescence microscopy for studying cellular and biomolecular dynamics

Journal Article Optics InfoBase Conference Papers · 2011 We have developed a microscope that simultaneously captures quantitative phase measurements and two distinct fluorescence images on a single camera. This microscope is an effective tool for investigating cellular dynamics with molecular specificity. © 2011 ... Cite

Spectral-domain differential interference contrast microscopy

Journal Article Optics InfoBase Conference Papers · 2011 We present a novel imaging technique, termed spectral-domain DIC microscopy, for high-resolution quantitative measurement of optical pathlength gradients. Imaging of resolution target and live cardiomyocytes were demonstrated with 36pm resolution. © 2011 O ... Cite

Real-time quantitative phase and dual-channel fluorescence microscopy for studying cellular and biomolecular dynamics

Conference Optics InfoBase Conference Papers · January 1, 2011 We have developed a microscope that simultaneously captures quantitative phase measurements and two distinct fluorescence images on a single camera. This microscope is an effective tool for investigating cellular dynamics with molecular specificity. © 2011 ... Cite

Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy

Conference Optics InfoBase Conference Papers · January 1, 2011 We have developed a novel microscope combining quantitative phase and fluorescence microscopy to perform quantitative analysis of dynamic cardiomyocyte contraction. Phase-based parameters are informed by molecular specificity of fluorescence images. © 2011 ... Cite

Spectral-domain differential interference contrast microscopy

Conference Optics InfoBase Conference Papers · January 1, 2011 We present a novel imaging technique, termed spectral-domain DIC microscopy, for high-resolution quantitative measurement of optical pathlength gradients. Imaging of resolution target and live cardiomyocytes were demonstrated with 36pm resolution. © 2011 O ... Cite

Spectral-domain differential interference contrast microscopy

Conference Optics InfoBase Conference Papers · January 1, 2011 We present a novel imaging technique, termed spectral-domain DIC microscopy, for high-resolution quantitative measurement of optical pathlength gradients. Imaging of resolution target and live cardiomyocytes were demonstrated with 36pm resolution. © 2011 O ... Full text Cite

Real-time quantitative phase and dual-channel fluorescence microscopy for studying cellular and biomolecular dynamics

Conference Optics InfoBase Conference Papers · January 1, 2011 We have developed a microscope that simultaneously captures quantitative phase measurements and two distinct fluorescence images on a single camera. This microscope is an effective tool for investigating cellular dynamics with molecular specificity. © 2011 ... Full text Cite

Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy

Conference Optics InfoBase Conference Papers · January 1, 2011 We have developed a novel microscope combining quantitative phase and fluorescence microscopy to perform quantitative analysis of dynamic cardiomyocyte contraction. Phase-based parameters are informed by molecular specificity of fluorescence images. © 2011 ... Full text Cite

Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress.

Journal Article Biophysical journal · December 2010 Late outgrowth endothelial progenitor cells (EPCs) represent a promising cell source for rapid reendothelialization of damaged vasculature after expansion ex vivo and injection into the bloodstream. We characterized the dynamic adhesion of umbilical-cord-b ... Full text Cite

Human umbilical cord blood-derived endothelial cells reendothelialize vein grafts and prevent thrombosis.

Journal Article Arterioscler Thromb Vasc Biol · November 2010 OBJECTIVE: To accelerate vein graft reendothelialization and reduce vein graft thrombosis by infusing human umbilical cord blood-derived endothelial cells (hCB-ECs) because loss of endothelium contributes to vein graft thrombosis and neointimal hyperplasia ... Full text Open Access Link to item Cite

Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy.

Journal Article Biomedical optics express · August 2010 We apply wide-field interferometric microscopy techniques to acquire quantitative phase profiles of ventricular cardiomyocytes in vitro during their rapid contraction with high temporal and spatial resolution. The whole-cell phase profiles are analyzed to ... Full text Open Access Cite

A method to replicate the microstructure of heart tissue in vitro using DTMRI-based cell micropatterning.

Journal Article Annals of biomedical engineering · December 2009 A novel cell culture methodology is described in which diffusion tensor magnetic resonance imaging (DTMRI) and cell micropatterning are combined to fabricate cell monolayers that replicate realistic cross-sectional tissue structure. As a proof-of-principle ... Full text Cite

Preface

Journal Article Annual Review of Nursing Education · December 1, 2008 Cite

ApoA-I induced CD31 in bone marrow-derived vascular progenitor cells increases adhesion: implications for vascular repair.

Journal Article Biochimica et biophysica acta · November 2008 Transgenic over expression of apolipoprotein A-I (ApoA-I) the major structural apolipoprotein of HDL appears to convey the most consistent and strongest anti atherogenic effect observed in animal models so far. We tested the hypothesis that ApoA-I mediates ... Full text Cite

Increased activity of phosphatase PP2A in the presence of the PlA2 polymorphism of alphaIIbbeta3.

Journal Article Biochemical and biophysical research communications · February 2008 Polymorphisms in alphaIIbbeta3 are important genetic factors that alter platelet biology and have been associated with susceptibility to thromboembolic disorders. To define the molecular mechanisms that lead to variance in thrombotic diathesis dictated by ... Full text Cite

Reactive oxygen species in vascular endothelial cell motility. Roles of NAD(P)H oxidase and Rac1.

Journal Article Cardiovascular research · July 2006 Reactive oxygen species (ROS) are acknowledged generally to be multi-faceted regulators of cellular functions that trigger various pathological states when present chronically or transiently at non-physiologically high levels. Here we focus on the physiolo ... Full text Cite

CD40 ligand: not bad to the bone (marrow), after all.

Journal Article Arteriosclerosis, thrombosis, and vascular biology · June 2005 Full text Cite

Mechanistic analysis of the Saccharomyces cerevisiae kinesin Kar3.

Journal Article The Journal of biological chemistry · December 2004 Kar3 is a minus-end-directed microtubule motor that is implicated in meiotic and mitotic spindle function in Saccharomyces cerevisiae. To date, the only truncated protein of Kar3 that has been reported to promote unidirectional movement in vitro is GSTKar3 ... Full text Cite

The minus end-directed motor Kar3 is required for coupling dynamic microtubule plus ends to the cortical shmoo tip in budding yeast.

Journal Article Current biology : CB · August 2003 The budding yeast shmoo tip is a model system for analyzing mechanisms coupling force production to microtubule plus-end polymerization/depolymerization. Dynamic plus ends of astral microtubules interact with the shmoo tip in mating yeast cells, positionin ... Full text Cite

New wrinkles in cytokinesis.

Journal Article Nature · January 1997 Full text Cite

Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends.

Journal Article EMBO J · June 1, 1994 Mutants of the yeast Kar3 protein are defective in nuclear fusion, or karyogamy, during mating and show slow mitotic growth, indicating a requirement for the protein both during mating and in mitosis. DNA sequence analysis predicts that Kar3 is a microtubu ... Full text Link to item Cite

Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein.

Journal Article The Journal of cell biology · February 1994 The Kar3 protein (Kar3p), a protein related to kinesin heavy chain, and the Cik1 protein (Cik1p) appear to participate in the same cellular processes in S. cerevisiae. Phenotypic analysis of mutants indicates that both CIK1 and KAR3 participate in spindle ... Full text Cite

Unravelling the tangled web at the microtubule-organizing center.

Journal Article Current opinion in cell biology · February 1993 The last year has seen dramatic progress in the use of genetic and biochemical approaches to identify microtubule-organizing center components. The use of vertebrate and invertebrate egg extracts has allowed the development of novel assays for centrosome d ... Full text Cite

Phosphorylation of myosin-II regulatory light chain by cyclin-p34cdc2: a mechanism for the timing of cytokinesis.

Journal Article The Journal of cell biology · August 1992 To understand how cytokinesis is regulated during mitosis, we tested cyclin-p34cdc2 for myosin-II kinase activity, and investigated the mitotic-specific phosphorylation of myosin-II in lysates of Xenopus eggs. Purified cyclin-p34cdc2 phosphorylated the reg ... Full text Cite

Cytokinesis.

Journal Article Current opinion in cell biology · February 1992 The actomyosin contractile-ring mechanism remains the paradigm for cytokinesis after 20 years of experimental testing. Recent evidence suggests that Ca2+ triggers the contraction and that cell-cycle kinases regulate the timing of cytokinesis. New work is r ... Full text Cite

A p34cdc2‐Containing Kinase Phosphorylates Myosin Regulatory Light Chain

Journal Article Annals of the New York Academy of Sciences · January 1, 1990 Full text Cite

Actin and myosin biochemistry in relation to cytokinesis.

Journal Article Annals of the New York Academy of Sciences · January 1990 Full text Cite