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Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers.

Publication ,  Journal Article
Fernandez, CE; Obi-onuoha, IC; Wallace, CS; Satterwhite, LL; Truskey, GA; Reichert, WM
Published in: Acta biomaterialia
February 2014

Patients with coronary artery disease (CAD) are the primary candidates to receive small-diameter tissue-engineered blood vessels (TEBVs). Peripheral blood derived endothelial progenitor cells (EPCs) from CAD patients (CAD EPCs) represent a minimally invasive source of autologous cells for TEBV endothelialization. We have previously shown that human CAD EPCs are highly proliferative and express many of the hallmarks of mature and healthy endothelial cells; however, their behavior on stromal cells that comprise the media of TEBVs has not yet been evaluated. Primary CAD EPCs or control human aortic endothelial cells (HAECs) were seeded over confluent, quiescent layers of human smooth muscle cells (SMCs) using a direct co-culture model. The percent coverage, adhesion strength, alignment under flow and generation of flow-induced nitric oxide of the seeded CAD EPCs were compared to that of HAECs. The integrin-binding profile of CAD EPCs was also evaluated over a layer of confluent, quiescent SMCs. Direct comparison of our CAD EPC results to analogous co-culture studies with cord blood EPCs show that both types of blood-derived EPCs are viable options for endothelialization of TEBVs.

Duke Scholars

Published In

Acta biomaterialia

DOI

EISSN

1878-7568

ISSN

1742-7061

Publication Date

February 2014

Volume

10

Issue

2

Start / End Page

893 / 900

Related Subject Headings

  • Stromal Cells
  • Stress, Mechanical
  • Stem Cells
  • Rheology
  • Nitrites
  • Integrins
  • Humans
  • Endothelium
  • Endothelial Cells
  • Coronary Artery Disease
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fernandez, C. E., Obi-onuoha, I. C., Wallace, C. S., Satterwhite, L. L., Truskey, G. A., & Reichert, W. M. (2014). Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers. Acta Biomaterialia, 10(2), 893–900. https://doi.org/10.1016/j.actbio.2013.10.004
Fernandez, Cristina E., Izundu C. Obi-onuoha, Charles S. Wallace, Lisa L. Satterwhite, George A. Truskey, and William M. Reichert. “Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers.Acta Biomaterialia 10, no. 2 (February 2014): 893–900. https://doi.org/10.1016/j.actbio.2013.10.004.
Fernandez CE, Obi-onuoha IC, Wallace CS, Satterwhite LL, Truskey GA, Reichert WM. Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers. Acta biomaterialia. 2014 Feb;10(2):893–900.
Fernandez, Cristina E., et al. “Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers.Acta Biomaterialia, vol. 10, no. 2, Feb. 2014, pp. 893–900. Epmc, doi:10.1016/j.actbio.2013.10.004.
Fernandez CE, Obi-onuoha IC, Wallace CS, Satterwhite LL, Truskey GA, Reichert WM. Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers. Acta biomaterialia. 2014 Feb;10(2):893–900.
Journal cover image

Published In

Acta biomaterialia

DOI

EISSN

1878-7568

ISSN

1742-7061

Publication Date

February 2014

Volume

10

Issue

2

Start / End Page

893 / 900

Related Subject Headings

  • Stromal Cells
  • Stress, Mechanical
  • Stem Cells
  • Rheology
  • Nitrites
  • Integrins
  • Humans
  • Endothelium
  • Endothelial Cells
  • Coronary Artery Disease