Monty Hughes Jr. | Scholars@Duke

Monty Hughes Jr.
Assistant Professor in Surgery

Dr. Hughes received his Ph.D. from the Medical University of South Carolina and was a post doc at both the University of North Carolina at Chapel Hill and NIH. He then joined the faculty of the University of North Carolina at Charlotte where he rose to the rank of Associate Professor (with tenure). Following a brief stint as the director of the biology division of a start-up pharmaceutical company, he joined forces with Dr. Purves at the Medical University of South Carolina to begin this lab focused on benign urinary disorders. Dr. Hughes has been at Duke since 2015. He is currently an Assistant Professor working within the Department of Surgery and Division of Urology. He serves as the Director of the Urinary Dysfunction Laboratory which studies the role of inflammation in disorders such as bladder outlet obstruction and diabetic bladder dysfunction. In association with Dr. J Todd Purves, this lab has been instrumental in demonstrating the central importance of the NLRP3 inflammasome in sensing the biochemical stressors associated with these disorders and translating them into an inflammatory signal. This signal is ultimately responsible for changes in voiding function, denervation and fibrosis.

Current Research Interests

Our lab focuses on projects that have direct relevance to disorders that are regularly encountered by practicing urologists in the clinic. We are particularly interested in benign urologic disease caused by inflammation in the bladder. We have shown that nod-like receptors (NLRs) and the inflammasomes they form are present in the bladder epithelia where they serve as sensors of sterile damage and potentially bacterial infection. Upon activation, these structures mature the pro-inflammatory cytokines IL-1β and IL-18, which are released and trigger inflammation and lower urinary tract symptoms, such as increased voiding frequency.

Inflammasome formation/activation during bladder outlet obstruction (such as during benign prostatic hyperplasia or BPH) and diabetes appears to be a major contributor to urinary dysfunction in those patient populations. Consequently, these two disorders are intensely under study in our lab. A thorough understanding of the inflammasome system in the bladder, while learning to manipulate this system for therapeutic advantage, is the main pillar of our lab.

Current Appointments & Affiliations

Assistant Professor in Surgery, Surgery, Urology, Surgery 2021

Contact Information

383 MSRB 1, Durham, NC 27710
383 MSRB 1, Durham, NC 27710
monty.hughes@duke.edu (843) 709-2128
Lab website

Background
Education, Training, & Certifications
- Senior Research Scientist, Surgery / Urology, Duke University School of Medicine 2015 - 2019
- Ph.D., Medical University of South Carolina 1992
- B.S., Clemson University 1987
Duke Appointment History
- Assistant Professor in Surgery, Surgery, Urology, Surgery 2020
Academic Positions Outside Duke
- Adjunct Associate Professor, Clemson University. 2016 - 2023
- Staff Scientist II, Medical University of South Carolina. 2010 - 2015
- Director of Biology, Argolyn Biosciences. 2007 - 2009
- Visiting Associate Professor, Medical University of South Carolina. 2007 - 2010
- Associate Professor, University of North Carolina at Charlotte. 2005 - 2008
- Assistant Professor , University of North Carolina at Charlotte. 1998 - 2005
Expertise
Subject Headings
Research
Selected Grants
- Inflammasome Mediated Inflammation in Diabetic Bladder Dysfunction awarded by National Institutes of Health 2019 - 2024
- Loss of Bladder Control Following Recurrent Infections awarded by National Institutes of Health 2020 - 2024
- Duke KURe Program awarded by National Institutes of Health 2013 - 2023
- Aberrant remodeling of bladder following infection awarded by National Institutes of Health 2019 - 2023
- Inflammasomes Mediate Inflammation in Bladder Outlet Obstruction awarded by National Institutes of Health 2015 - 2020
- The Mechanism of Inflammation in Diabetic Bladder Dysfunction awarded by American Urological Association Foundation 2016 - 2017
Publications & Artistic Works
Selected Publications
- Books
  - Hughes, F. M., and J. A. Cidlowski. Apoptotic nuclease assays. Vol. 322, 2000. https://doi.org/10.1016/s0076-6879(00)22007-7.
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- Academic Articles
  - Hughes, Francis M., Nathan A. Hirshman, Hamza A. Malick, Simon W. White, Huixia Jin, Shelby N. Harper, and J Todd Purves. “A possible mechanism underlying mood disorders associated with LUTS: Chronic bladder outlet obstruction causes NLRP3-dependent inflammation in the hippocampus and depressive behavior in rats.” Neurourol Urodyn 39, no. 6 (August 2020): 1700–1707. https://doi.org/10.1002/nau.24448.
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  - Wu, Jianxuan, Byron W. Hayes, Cassandra Phoenix, Gustavo Sosa Macias, Yuxuan Miao, Hae Woong Choi, Francis M. Hughes, J. Todd Purves, R. Lee Reinhardt, and Soman N. Abraham. “A highly polarized TH2 bladder response to infection promotes epithelial repair at the expense of preventing new infections.” Nat Immunol 21, no. 6 (June 2020): 671–83. https://doi.org/10.1038/s41590-020-0688-3.
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  - Hirshman, Nathan A., Francis M. Hughes, Huixia Jin, William T. Harrison, Simon W. White, Isabelle Doan, Shelby N. Harper, Patrick D. Leidig, and J Todd Purves. “Cyclophosphamide-induced cystitis results in NLRP3-mediated inflammation in the hippocampus and symptoms of depression in rats.” Am J Physiol Renal Physiol 318, no. 2 (February 1, 2020): F354–62. https://doi.org/10.1152/ajprenal.00408.2019.
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  - Sivaraman, Srikanth, Nicholas Amoroso, Xinzhu Gu, J Todd Purves, Francis M. Hughes, William R. Wagner, and Jiro Nagatomi. “Evaluation of Poly (Carbonate-Urethane) Urea (PCUU) Scaffolds for Urinary Bladder Tissue Engineering.” Ann Biomed Eng 47, no. 3 (March 2019): 891–901. https://doi.org/10.1007/s10439-018-02182-0.
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  - Hughes, Francis M., Nathan A. Hirshman, Brian M. Inouye, Huixia Jin, Eloise W. Stanton, Chloe E. Yun, Leah G. Davis, Jonathan C. Routh, and J Todd Purves. “NLRP3 Promotes Diabetic Bladder Dysfunction and Changes in Symptom-Specific Bladder Innervation.” Diabetes 68, no. 2 (February 2019): 430–40. https://doi.org/10.2337/db18-0845.
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  - Harper, Shelby N., Patrick D. Leidig, Francis M. Hughes, Huixia Jin, and J Todd Purves. “Calcium Pyrophosphate And Monosodium Urate Activate The NLRP3 Inflammasome Within Bladder Urothelium Via Reactive Oxygen Species And TXNIP.” Res Rep Urol 11 (2019): 319–25. https://doi.org/10.2147/RRU.S225767.
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  - Hughes, Francis M., Stephanie J. Sexton, Patrick D. Ledig, Chloe E. Yun, Huixia Jin, and J Todd Purves. “Bladder decompensation and reduction in nerve density in a rat model of chronic bladder outlet obstruction are attenuated with the NLRP3 inhibitor glyburide.” Am J Physiol Renal Physiol 316, no. 1 (January 1, 2019): F113–20. https://doi.org/10.1152/ajprenal.00400.2018.
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  - Dunton, Cody L., J Todd Purves, Francis M. Hughes, Huixia Jin, and Jiro Nagatomi. “Elevated hydrostatic pressure stimulates ATP release which mediates activation of the NLRP3 inflammasome via P2X4 in rat urothelial cells.” Int Urol Nephrol 50, no. 9 (September 2018): 1607–17. https://doi.org/10.1007/s11255-018-1948-0.
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  - Lütolf, Robin, Francis M. Hughes, Brian M. Inouye, Huixia Jin, Jennifer C. McMains, Elena S. Pak, Johanna L. Hannan, and J Todd Purves. “NLRP3/IL-1β mediates denervation during bladder outlet obstruction in rats.” Neurourol Urodyn 37, no. 3 (March 2018): 952–59. https://doi.org/10.1002/nau.23419.
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  - Inouye, Brian M., Francis M. Hughes, Stephanie J. Sexton, and J Todd Purves. “The Emerging Role of Inflammasomes as Central Mediators in Inflammatory Bladder Pathology.” Curr Urol 11, no. 2 (February 2018): 57–72. https://doi.org/10.1159/000447196.
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  - Inouye, Brian M., Francis M. Hughes, Huixia Jin, Robin Lütolf, Kunal C. Potnis, Jonathan C. Routh, Douglas C. Rouse, Wen-Chi Foo, and J Todd Purves. “Diabetic bladder dysfunction is associated with bladder inflammation triggered through hyperglycemia, not polyuria.” Res Rep Urol 10 (2018): 219–25. https://doi.org/10.2147/RRU.S177633.
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  - Hughes, Francis M., Stephanie J. Sexton, Huixia Jin, Vihasa Govada, and J Todd Purves. “Bladder fibrosis during outlet obstruction is triggered through the NLRP3 inflammasome and the production of IL-1β.” Am J Physiol Renal Physiol 313, no. 3 (September 1, 2017): F603–10. https://doi.org/10.1152/ajprenal.00128.2017.
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  - Purves, J Todd, Laura Spruill, Eric Rovner, Elyse Borisko, Alden McCants, Elizabeth Mugo, Ainsley Wingard, Thomas C. Trusk, Thierry Bacro, and Francis M. Hughes. “A three dimensional nerve map of human bladder trigone.” Neurourol Urodyn 36, no. 4 (April 2017): 1015–19. https://doi.org/10.1002/nau.23049.
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  - Purves, J Todd, and F Monty Hughes. “Inflammasomes in the urinary tract: a disease-based review.” Am J Physiol Renal Physiol 311, no. 4 (October 1, 2016): F653–62. https://doi.org/10.1152/ajprenal.00607.2015.
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  - Hughes, Francis M., Hayden M. Hill, Case M. Wood, Andrew T. Edmondson, Aliya Dumas, Wen-Chi Foo, James M. Oelsen, Goran Rac, and J Todd Purves. “The NLRP3 Inflammasome Mediates Inflammation Produced by Bladder Outlet Obstruction.” J Urol 195, no. 5 (May 2016): 1598–1605. https://doi.org/10.1016/j.juro.2015.12.068.
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  - Hughes, Francis M., David P. Turner, and J. Todd Purves. “The potential repertoire of the innate immune system in the bladder: expression of pattern recognition receptors in the rat bladder and a rat urothelial cell line (MYP3 cells).” Int Urol Nephrol 47, no. 12 (December 2015): 1953–64. https://doi.org/10.1007/s11255-015-1126-6.
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  - Shunmugavel, Anandakumar, Mushfiquddin Khan, Francis M. Hughes, J Todd Purves, Avtar Singh, and Inderjit Singh. “S-Nitrosoglutathione protects the spinal bladder: novel therapeutic approach to post-spinal cord injury bladder remodeling.” Neurourol Urodyn 34, no. 6 (August 2015): 519–26. https://doi.org/10.1002/nau.22619.
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  - Schneider, Marc P., Francis M. Hughes, Anne K. Engmann, J Todd Purves, Hansjörg Kasper, Marco Tedaldi, Laura S. Spruill, Miriam Gullo, Martin E. Schwab, and Thomas M. Kessler. “A novel urodynamic model for lower urinary tract assessment in awake rats.” Bju Int 115 Suppl 6 (April 2015): 8–15. https://doi.org/10.1111/bju.13039.
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  - Hughes, Francis M., Nivardo P. Vivar, James G. Kennis, Jeffery D. Pratt-Thomas, Danielle W. Lowe, Brooke E. Shaner, Paul J. Nietert, Laura S. Spruill, and J Todd Purves. “Inflammasomes are important mediators of cyclophosphamide-induced bladder inflammation.” Am J Physiol Renal Physiol 306, no. 3 (February 1, 2014): F299–308. https://doi.org/10.1152/ajprenal.00297.2013.
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  - Hughes, F. M., B. E. Shaner, J. O. Brower, R. J. Woods, and T. A. Dix. “Development of a peptide-derived orally-active kappa-opioid receptor agonist targeting peripheral pain.” Open Medicinal Chemistry Journal 7, no. 1 (November 26, 2013): 16–22. https://doi.org/10.2174/1874104501307010016.
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  - Hughes, Francis M., Paige McKeithan, Justin Ellett, Kent E. Armeson, and J Todd Purves. “Simvastatin suppresses cyclophosphamide-induced changes in urodynamics and bladder inflammation.” Urology 81, no. 1 (January 2013): 209.e9-209.14. https://doi.org/10.1016/j.urology.2012.08.041.
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  - Foster, Brent, Snimar Grewal, Ondrea Graves, Francis M. Hughes, and Inna M. Sokolova. “Copper exposure affects hemocyte apoptosis and Perkinsus marinus infection in eastern oysters Crassostrea virginica (Gmelin).” Fish Shellfish Immunol 31, no. 2 (August 2011): 341–49. https://doi.org/10.1016/j.fsi.2011.05.024.
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  - Hughes, Francis M., Brent Foster, Snimar Grewal, and Inna M. Sokolova. “Apoptosis as a host defense mechanism in Crassostrea virginica and its modulation by Perkinsus marinus.” Fish Shellfish Immunol 29, no. 2 (August 2010): 247–57. https://doi.org/10.1016/j.fsi.2010.03.003.
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  - Hughes, Francis M., Brooke E. Shaner, Lisa A. May, Lyndsay Zotian, Justin O. Brower, R Jeremy Woods, Michael Cash, et al. “Identification and functional characterization of a stable, centrally active derivative of the neurotensin (8-13) fragment as a potential first-in-class analgesic.” J Med Chem 53, no. 12 (June 24, 2010): 4623–32. https://doi.org/10.1021/jm100092s.
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  - Roach, Joseph D., Grant T. Aguinaldo, Kaumudi Jonnalagadda, Francis M. Hughes, and Bryan L. Spangelo. “Gamma-aminobutyric acid inhibits synergistic interleukin-6 release but not transcriptional activation in astrocytoma cells.” Neuroimmunomodulation 15, no. 2 (2008): 117–24. https://doi.org/10.1159/000148194.
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  - Jablonski, Elizabeth M., M Adrian Mattocks, Eugene Sokolov, Leonidas G. Koniaris, Francis M. Hughes, Nelson Fausto, Robert H. Pierce, and Iain H. McKillop. “Decreased aquaporin expression leads to increased resistance to apoptosis in hepatocellular carcinoma.” Cancer Lett 250, no. 1 (May 18, 2007): 36–46. https://doi.org/10.1016/j.canlet.2006.09.013.
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  - Jablonski, Elizabeth M., and Francis M. Hughes. “The potential role of caveolin-1 in inhibition of aquaporins during the AVD.” Biol Cell 98, no. 1 (January 2006): 33–42. https://doi.org/10.1042/BC20040131.
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  - Sokolova, I. M., S. Evans, and F. M. Hughes. “Cadmium-induced apoptosis in oyster hemocytes involves disturbance of cellular energy balance but no mitochondrial permeability transition.” J Exp Biol 207, no. Pt 19 (September 2004): 3369–80. https://doi.org/10.1242/jeb.01152.
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  - Jablonski, Elizabeth M., Ashley N. Webb, Nisha A. McConnell, Marcus C. Riley, and Francis M. Hughes. “Plasma membrane aquaporin activity can affect the rate of apoptosis but is inhibited after apoptotic volume decrease.” Am J Physiol Cell Physiol 286, no. 4 (April 2004): C975–85. https://doi.org/10.1152/ajpcell.00180.2003.
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  - Jablonski, Elizabeth M., Nisha A. McConnell, Francis M. Hughes, and Yvette M. Huet-Hudson. “Estrogen regulation of aquaporins in the mouse uterus: potential roles in uterine water movement.” Biol Reprod 69, no. 5 (November 2003): 1481–87. https://doi.org/10.1095/biolreprod.103.019927.
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  - Marriott, Ian, Francis M. Hughes, and Kenneth L. Bost. “Bacterial infection of osteoblasts induces interleukin-1beta and interleukin-18 transcription but not protein synthesis.” J Interferon Cytokine Res 22, no. 10 (October 2002): 1049–55. https://doi.org/10.1089/107999002760624288.
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  - McConnell, Nisha A., Raheela S. Yunus, Stephen A. Gross, Kenneth L. Bost, Mark G. Clemens, and Francis M. Hughes. “Water permeability of an ovarian antral follicle is predominantly transcellular and mediated by aquaporins.” Endocrinology 143, no. 8 (August 2002): 2905–12. https://doi.org/10.1210/endo.143.8.8953.
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  - Gross, S. A., J. M. Newton, and F. M. Hughes. “Decreased intracellular potassium levels underlie increased progesterone synthesis during ovarian follicular atresia.” Biol Reprod 64, no. 6 (June 2001): 1755–60. https://doi.org/10.1095/biolreprod64.6.1755.
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  - Perez, G. I., D. V. Maravei, A. M. Trbovich, J. A. Cidlowski, J. L. Tilly, and F. M. Hughes. “Identification of potassium-dependent and -independent components of the apoptotic machinery in mouse ovarian germ cells and granulosa cells.” Biol Reprod 63, no. 5 (November 2000): 1358–69. https://doi.org/10.1095/biolreprod63.5.1358.
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  - Mann, C. L., F. M. Hughes, and J. A. Cidlowski. “Delineation of the signaling pathways involved in glucocorticoid-induced and spontaneous apoptosis of rat thymocytes.” Endocrinology 141, no. 2 (February 2000): 528–38. https://doi.org/10.1210/endo.141.2.7314.
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  - Montague, J. W., C. D. Bortner, F. M. Hughes, and J. A. Cidlowski. “A necessary role for reduced intracellular potassium during the DNA degradation phase of apoptosis.” Steroids 64, no. 9 (September 1999): 563–69. https://doi.org/10.1016/s0039-128x(99)00034-3.
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  - Hughes, F. M., R. B. Evans-Storms, and J. A. Cidlowski. “Evidence that non-caspase proteases are required for chromatin degradation during apoptosis.” Cell Death Differ 5, no. 12 (December 1998): 1017–27. https://doi.org/10.1038/sj.cdd.4400418.
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  - Adachi, H., A. Adams, F. M. Hughes, J. Zhang, J. A. Cidlowski, and A. M. Jetten. “Induction of apoptosis by the novel retinoid AHPN in human T-cell lymphoma cells involves caspase-dependent and independent pathways.” Cell Death Differ 5, no. 11 (November 1998): 973–83. https://doi.org/10.1038/sj.cdd.4400445.
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  - Spangelo, B. L., D. D. Farrimond, M. Thapa, C. M. Bulathsinghala, K. L. Bowman, A. Sareh, F. M. Hughes, A. L. Goldstein, and M. Badamchian. “Thymosin fraction 5 inhibits the proliferation of the rat neuroendocrine MMQ pituitary adenoma and C6 glioma cell lines in vitro.” Endocrinology 139, no. 4 (April 1998): 2155–62. https://doi.org/10.1210/endo.139.4.5935.
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  - Trbovich, A. M., F. M. Hughes, G. I. Perez, K. Kugu, K. I. Tilly, J. A. Cidlowski, and J. L. Tilly. “High and low molecular weight DNA cleavage in ovarian granulosa cells: characterization and protease modulation in intact cells and in cell-free nuclear autodigestion assays.” Cell Death Differ 5, no. 1 (January 1998): 38–49. https://doi.org/10.1038/sj.cdd.4400299.
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  - Bortner, C. D., F. M. Hughes, and J. A. Cidlowski. “A primary role for K+ and Na+ efflux in the activation of apoptosis.” J Biol Chem 272, no. 51 (December 19, 1997): 32436–42. https://doi.org/10.1074/jbc.272.51.32436.
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  - Hughes, F. M., C. D. Bortner, G. D. Purdy, and J. A. Cidlowski. “Intracellular K+ suppresses the activation of apoptosis in lymphocytes.” J Biol Chem 272, no. 48 (November 28, 1997): 30567–76. https://doi.org/10.1074/jbc.272.48.30567.
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  - Huang, S. T. J., F. M. Hughes, and J. A. Cidlowski. “Candidate nucleases responsible for genomic degradation during apoptosis.” Comments on Toxicology 5, no. 6 (June 30, 1997): 555–69.
  - Bian, X., F. M. Hughes, Y. Huang, J. A. Cidlowski, and J. W. Putney. “Roles of cytoplasmic Ca2+ and intracellular Ca2+ stores in induction and suppression of apoptosis in S49 cells.” Am J Physiol 272, no. 4 Pt 1 (April 1997): C1241–49. https://doi.org/10.1152/ajpcell.1997.272.4.C1241.
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  - Hughes, F. M., and J. A. Cidlowski. “Utilization of an in vitro assay to evaluate chromatin degradation by candidate apoptotic nucleases.” Cell Death Differ 4, no. 3 (April 1997): 200–208. https://doi.org/10.1038/sj.cdd.4400221.
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  - Montague, J. W., F. M. Hughes, and J. A. Cidlowski. “Native recombinant cyclophilins A, B, and C degrade DNA independently of peptidylprolyl cis-trans-isomerase activity. Potential roles of cyclophilins in apoptosis.” J Biol Chem 272, no. 10 (March 7, 1997): 6677–84. https://doi.org/10.1074/jbc.272.10.6677.
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  - Hughes, F. M., G. D. Purdy, and J. A. Cidlowski. “Intracellular potassium levels inhibit apoptotic dna degradation.” Faseb Journal 10, no. 6 (December 1, 1996).
  - Cidlowski, J. A., K. L. King, R. B. Evans-Storms, J. W. Montague, C. D. Bortner, and F. M. Hughes. “The biochemistry and molecular biology of glucocorticoid-induced apoptosis in the immune system.” Recent Prog Horm Res 51 (1996): 457–90.
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  - Hughes, F. M., and J. A. Cidlowski. “Apoptotic DNA degradation: Evidence for novel enzymes.” Cell Death and Differentiation 1, no. 1 (December 1, 1994): 11–17.
  - HUGHES, F. M., Y. Y. FONG, and W. C. GOROSPE. “INTERLEUKIN-6 STIMULATES APOPTOSIS IN FSH-STIMULATED RAT GRANULOSA-CELLS IN-VITRO - DEVELOPMENT AND UTILIZATION OF AN IN-VITRO MODEL.” Endocrine 2, no. 11 (November 1, 1994): 997–1002.
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  - Hughes, F. M., and J. A. Cidlowski. “Regulation of apoptosis in S49 cells.” J Steroid Biochem Mol Biol 49, no. 4–6 (June 1994): 303–10. https://doi.org/10.1016/0960-0760(94)90272-0.
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  - Johnson, K. M., F. M. Hughes, Y. Y. Fong, R. S. Mathur, H. O. Williamson, and W. C. Gorospe. “Effects of atrial natriuretic peptide on rat ovarian granulosa cell steroidogenesis in vitro.” Am J Reprod Immunol 31, no. 2–3 (March 1994): 163–68. https://doi.org/10.1111/j.1600-0897.1994.tb00861.x.
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  - Gorospe, W. C., F. M. Hughes, and B. L. Spangelo. “Interleukin-6: effects on and production by rat granulosa cells in vitro.” Endocrinology 130, no. 3 (March 1992): 1750–52. https://doi.org/10.1210/endo.130.3.1537322.
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  - Hughes, F. M., and W. C. Gorospe. “Biochemical identification of apoptosis (programmed cell death) in granulosa cells: evidence for a potential mechanism underlying follicular atresia.” Endocrinology 129, no. 5 (November 1991): 2415–22. https://doi.org/10.1210/endo-129-5-2415.
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  - Hughes, F. M., C. M. Pringle, and W. C. Gorospe. “Production of progestin-stimulatory factor(s) by enriched populations of rat T and B lymphocytes.” Biol Reprod 44, no. 5 (May 1991): 922–26. https://doi.org/10.1095/biolreprod44.5.922.
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  - Hughes, F. M., T. A. Lane, T. T. Chen, and W. C. Gorospe. “Effects of cytokines on porcine granulosa cell steroidogenesis in vitro.” Biol Reprod 43, no. 5 (November 1990): 812–17. https://doi.org/10.1095/biolreprod43.5.812.
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- Book Sections
  - MacLeod, R. M., F. M. Hughes, W. C. Gorospe, and B. L. Spangelo. “Synthesis, Release, and Actions of Interleukin 6 in Neuroendocrine Tissues: Methods and Overview.” In Methods in Neurosciences, 17:3–15, 1993. https://doi.org/10.1016/S1043-9471(13)70006-5.
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- Conference Papers
  - Purves, Todd, Nathan Hirshman, Huixia Jin, Isabelle Doan, Simon White, Shelby Harper, and Francis Hughes. “CYCLOPHOSPHAMIDE-INDUCED CYSTITIS TRIGGERS NLRP3-DEPENDENT NEUROINFLAMMATION IN THE HIPPOCAMPUS AND DEPRESSION IN RATS.” In Journal of Urology, 201:E688–E688. LIPPINCOTT WILLIAMS & WILKINS, 2019.
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  - Hirshman, N., F. Hughes, H. Jin, and T. Purves. “BEST IN CATEGORY PRIZE "NEUROUROLOGY" SYMPTOMS OF DIABETIC BLADDER DYSFUNCTION MAY BE EXPLAINED BY SPECIFIC NLRP3-INDUCED CHANGES IN BLADDER AFFERENT NERVES.” In Neurourology and Urodynamics, 37:S164–66. WILEY, 2018.
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  - Hughes, Francis, Brian Inouye, Eloise Stanton, Chloe Yun, Huixia Jin, and J. Purves. “NLRP3 INFLAMMASOME MEDIATED INFLAMMATION AND DENERVATION ARE CRITICAL PROCESSES IN THE DEVELOPMENT OF DIABETIC BLADDER DYSFUNCTION.” In Journal of Urology, 199:E501–E501. ELSEVIER SCIENCE INC, 2018.
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  - Hughes, Francis, Stephanie Sexton, Patrick Leidig, Huixia Jin, and J. Purves. “PHARMACOLOGICAL INHIBITION OF THE NLRP3 INFLAMMASOME PREVENTS BLADDER DECOMPENSATION IN A RAT MODEL OF CHRONIC BLADDER OUTLET OBSTRUCTION.” In Journal of Urology, 199:E112–13. ELSEVIER SCIENCE INC, 2018.
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  - Leidig, Patrick, Francis Hughes, and J Todd Purves. “COMPONENTS OF URINARY STONES ACTIVATE THE NLRP3 INFLAMMASOME IN BLADDER UROTHELIUM.” In Journal of Urology, 199:E298–E298. ELSEVIER SCIENCE INC, 2018.
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  - Inouye, Brian M., Jillene M. Brooks, Danielle J. Degoski, Francis M. Hughes Jr., J Todd Purves, and Matthew O. Fraser. “MP85-02 ABERRANT BLADDER REFLEXES CAN DRIVE HIND LIMB LOCOMOTOR ACTIVITY FOLLOWING COMPLETE SUPRASACRAL SPINAL CORD INJURY.” In Journal of Urology, Vol. 197. Ovid Technologies (Wolters Kluwer Health), 2017. https://doi.org/10.1016/j.juro.2017.02.2664.
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  - Inouye, Brian, Francis M. Hughes, Robin Lütolf, Clay Rouse, Wen-Chi Foo, and J Todd Purves. “PD12-06 INFLAMMASOME ACTIVATION EARLY IN THE DEVELOPMENT OF DIABETIC BLADDER DYSFUNCTION.” In Journal of Urology, Vol. 197. Ovid Technologies (Wolters Kluwer Health), 2017. https://doi.org/10.1016/j.juro.2017.02.681.
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  - Lütolf, Robin, Huixia Jin, Francis Monty Hughes, and J Todd Purves. “MP23-20 NLRP3/IL-1β MEDIATES DENERVATION DURING BLADDER OUTLET OBSTRUCTION IN RATS.” In Journal of Urology, Vol. 197. Ovid Technologies (Wolters Kluwer Health), 2017. https://doi.org/10.1016/j.juro.2017.02.748.
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  - Inouye, Brian M., Jillene M. Brooks, Danielle J. Degoski, Francis M. Hughes, J Todd Purves, and Matthew O. Fraser. “ABERRANT BLADDER REFLEXES CAN DRIVE HIND LIMB LOCOMOTOR ACTIVITY FOLLOWING COMPLETE SUPRASACRAL SPINAL CORD INJURY.” In Neurourology and Urodynamics, 36:S20–S20. WILEY-BLACKWELL, 2017.
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  - Sivaraman, S., C. Ambrose, X. Gu, P. Todd, F. M. Hughes, W. Wagner, and J. Nagatomi. “Evaluation of Poly (carbonate-urethane) Urea (pcuu) as a Urinary Bladder Tissue Engineering Scaffold.” In Tissue Engineering Part A, 21:S173–74. MARY ANN LIEBERT, INC, 2015.
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  - Jablonski, Elizabeth, Ashley Webb, and Francis M. Hughes. “Water movement during apoptosis: a role for aquaporins in the apoptotic volume decrease (AVD).” In Adv Exp Med Biol, 559:179–88, 2004. https://doi.org/10.1007/0-387-23752-6_17.
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  - Rueda, B. R., E. M. Jablonski, E. Oliva, Y. Huet-Hudson, and F. M. Hughes. “Estrogen regulation of AQP-2 expression in human uterine epithelium.” In Biology of Reproduction, 192–192. SOC STUDY REPRODUCTION, 2004.
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  - Jablonski, E. M., N. A. McConnell, A. T. Motameni, Y. M. Huet-Hudson, and F. M. Hughes. “Estrogen regulation of the expression and function of AQPs in the mouse uterus.” In Biology of Reproduction, 68:117–18. SOC STUDY REPRODUCTION, 2003.
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  - McConnell, N. A., S. G. Yan, and F. M. Hughes. “FSH-induced aquaporin 8 expression in rat granulosa cells is mediated by a PKA-dependent mechanism.” In Biology of Reproduction, 68:113–14. SOC STUDY REPRODUCTION, 2003.
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  - Hughes, F. M., and J. A. Cidlowski. “Potassium is a critical regulator of apoptotic enzymes in vitro and in vivo.” In Adv Enzyme Regul, 39:157–71, 1999. https://doi.org/10.1016/s0065-2571(98)00010-7.
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  - Hughes, F. M., and J. A. Cidlowski. “Glucocorticoid-induced thymocyte apoptosis: protease-dependent activation of cell shrinkage and DNA degradation.” In J Steroid Biochem Mol Biol, 65:207–17, 1998. https://doi.org/10.1016/s0960-0760(97)00188-x.
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  - Tilly, J. L., J. A. Flaws, A. M. DeSanti, F. M. Hughes, K. I. Tilly, D. V. Maravei, A. M. Trbovich, J. A. Cidlowski, and A. N. Hirshfield. “Biochemical and morphological investigation of apoptosis and atresia in rat ovarian antral follicles incubated in vitro.” In Biology of Reproduction, 54:124–124. SOC STUDY REPRODUCTION, 1996.
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  - CARONLESLIE, L. A. M., J. W. MONTAGUE, H. YU, F. M. HUGHES, R. A. SCHWARTZMAN, and J. A. CIDLOWSKI. “CORTICOSTEROID INDUCED APOPTOSIS IN THE IMMUNE-SYSTEM - NOVEL MECHANISMS OF REGULATION.” In Journal of Cellular Biochemistry, 140–140. WILEY-LISS, 1993.
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Teaching & Mentoring
Advising & Mentoring
- Present
  Michael Odom - Post Doc
  Armand Allkanjari - Resident
  
  Previous
  Resident:
  Brent Nose
  Patrick Leidig
  Brian Inouye
  Stephanie Sexton
  
  PhD Students:
  Marc Schneider
  Elizabeth Jablonski
  Nisha McConnell
  Advisory committee for 8 additional PhD students
  
  Medical Students:
  Shelby Harper
  Nathan Hirshman
  Gordan Rac
  Hayden Hill
  Melissa Youssef
  Case Wood
  
  Masters Students:
  Robin Lutolf
  Snimer Grewal
  Swapna Ambati
  Ashley Webb
  Stephen Gross
  
  Undergraduate:
  39 students doing independent studies research
Teaching Activities
- Over 26 courses ranging from undergraduate cell biology to graduate level course

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Education, Training, & Certifications

Duke Appointment History

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Advising & Mentoring

Teaching Activities