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Efficiency, selectivity, and robustness of nucleocytoplasmic transport.

Publication ,  Journal Article
Zilman, A; Di Talia, S; Chait, BT; Rout, MP; Magnasco, MO
Published in: PLoS Comput Biol
July 2007

All materials enter or exit the cell nucleus through nuclear pore complexes (NPCs), efficient transport devices that combine high selectivity and throughput. NPC-associated proteins containing phenylalanine-glycine repeats (FG nups) have large, flexible, unstructured proteinaceous regions, and line the NPC. A central feature of NPC-mediated transport is the binding of cargo-carrying soluble transport factors to the unstructured regions of FG nups. Here, we model the dynamics of nucleocytoplasmic transport as diffusion in an effective potential resulting from the interaction of the transport factors with the flexible FG nups, using a minimal number of assumptions consistent with the most well-established structural and functional properties of NPC transport. We discuss how specific binding of transport factors to the FG nups facilitates transport, and how this binding and competition between transport factors and other macromolecules for binding sites and space inside the NPC accounts for the high selectivity of transport. We also account for why transport is relatively insensitive to changes in the number and distribution of FG nups in the NPC, providing an explanation for recent experiments where up to half the total mass of the FG nups has been deleted without abolishing transport. Our results suggest strategies for the creation of artificial nanomolecular sorting devices.

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Published In

PLoS Comput Biol

DOI

EISSN

1553-7358

Publication Date

July 2007

Volume

3

Issue

7

Start / End Page

e125

Location

United States

Related Subject Headings

  • Thermodynamics
  • Protein Structure, Tertiary
  • Protein Binding
  • Nuclear Pore Complex Proteins
  • Nuclear Pore
  • Models, Chemical
  • Models, Biological
  • Karyopherins
  • Energy Transfer
  • Diffusion
 

Citation

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Zilman, A., Di Talia, S., Chait, B. T., Rout, M. P., & Magnasco, M. O. (2007). Efficiency, selectivity, and robustness of nucleocytoplasmic transport. PLoS Comput Biol, 3(7), e125. https://doi.org/10.1371/journal.pcbi.0030125
Zilman, Anton, Stefano Di Talia, Brian T. Chait, Michael P. Rout, and Marcelo O. Magnasco. “Efficiency, selectivity, and robustness of nucleocytoplasmic transport.PLoS Comput Biol 3, no. 7 (July 2007): e125. https://doi.org/10.1371/journal.pcbi.0030125.
Zilman A, Di Talia S, Chait BT, Rout MP, Magnasco MO. Efficiency, selectivity, and robustness of nucleocytoplasmic transport. PLoS Comput Biol. 2007 Jul;3(7):e125.
Zilman, Anton, et al. “Efficiency, selectivity, and robustness of nucleocytoplasmic transport.PLoS Comput Biol, vol. 3, no. 7, July 2007, p. e125. Pubmed, doi:10.1371/journal.pcbi.0030125.
Zilman A, Di Talia S, Chait BT, Rout MP, Magnasco MO. Efficiency, selectivity, and robustness of nucleocytoplasmic transport. PLoS Comput Biol. 2007 Jul;3(7):e125.

Published In

PLoS Comput Biol

DOI

EISSN

1553-7358

Publication Date

July 2007

Volume

3

Issue

7

Start / End Page

e125

Location

United States

Related Subject Headings

  • Thermodynamics
  • Protein Structure, Tertiary
  • Protein Binding
  • Nuclear Pore Complex Proteins
  • Nuclear Pore
  • Models, Chemical
  • Models, Biological
  • Karyopherins
  • Energy Transfer
  • Diffusion