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Molecular dissection of gating in the ClC-2 chloride channel.

Publication ,  Journal Article
Jordt, SE; Jentsch, TJ
Published in: EMBO J
April 1, 1997

The ClC-2 chloride channel is probably involved in the regulation of cell volume and of neuronal excitability. Site-directed mutagenesis was used to understand ClC-2 activation in response to cell swelling, hyperpolarization and acidic extracellular pH. Similar to equivalent mutations in ClC-0, neutralizing Lys566 at the end of the transmembrane domains results in outward rectification and a shift in voltage dependence, but leaves the basic gating mechanism, including swelling activation, intact. In contrast, mutations in the cytoplasmic loop between transmembrane domains D7 and D8 abolish all three modes of activation by constitutively opening the channel without changing its pore properties. These effects resemble those observed with deletions of an amino-terminal inactivation domain, and suggest that it may act as its receptor. Such a 'ball-and-chain' type mechanism may act as a final pathway in the activation of ClC-2 elicited by several stimuli.

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Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

April 1, 1997

Volume

16

Issue

7

Start / End Page

1582 / 1592

Location

England

Related Subject Headings

  • Xenopus laevis
  • Recombinant Fusion Proteins
  • Rats
  • Protein Structure, Secondary
  • Point Mutation
  • Patch-Clamp Techniques
  • Oocytes
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Models, Structural
 

Citation

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Jordt, S. E., & Jentsch, T. J. (1997). Molecular dissection of gating in the ClC-2 chloride channel. EMBO J, 16(7), 1582–1592. https://doi.org/10.1093/emboj/16.7.1582
Jordt, S. E., and T. J. Jentsch. “Molecular dissection of gating in the ClC-2 chloride channel.EMBO J 16, no. 7 (April 1, 1997): 1582–92. https://doi.org/10.1093/emboj/16.7.1582.
Jordt SE, Jentsch TJ. Molecular dissection of gating in the ClC-2 chloride channel. EMBO J. 1997 Apr 1;16(7):1582–92.
Jordt, S. E., and T. J. Jentsch. “Molecular dissection of gating in the ClC-2 chloride channel.EMBO J, vol. 16, no. 7, Apr. 1997, pp. 1582–92. Pubmed, doi:10.1093/emboj/16.7.1582.
Jordt SE, Jentsch TJ. Molecular dissection of gating in the ClC-2 chloride channel. EMBO J. 1997 Apr 1;16(7):1582–1592.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

April 1, 1997

Volume

16

Issue

7

Start / End Page

1582 / 1592

Location

England

Related Subject Headings

  • Xenopus laevis
  • Recombinant Fusion Proteins
  • Rats
  • Protein Structure, Secondary
  • Point Mutation
  • Patch-Clamp Techniques
  • Oocytes
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Models, Structural